LOCUS       sequence-211494-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-xBE3-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1726..1843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1912..2123
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2149..2835
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             2884..6984
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 mutant with broad PAM
                     compatibility (Hu et al., 2018)"
                     /label=xCas9-3.7
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6997..7245
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7258..7278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7294..7350
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7351..7950
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7351..7370)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7847..7867
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7966..8554
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8019..8039)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8437..8448)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8626..8859
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8937..9058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8974..8993)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9028..9047
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9098..9233
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9253..9272)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9254..9272)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9282..9299)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9282..9298)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9291..9313)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9440..9895
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9527..9546)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9737..9758
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9921..10025
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10026..10094,10095..10886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10244..10263)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11057..11645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11546..11565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11799..11816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11933..11954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11969..11974,11975..11992,11993..11999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12007..12023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12012..12034
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12031..12047
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12031..12047
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12066..12086
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12068..12086
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccagac cacaatttta aaagaaaagg
     1741 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1801 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1861 cagcagagat ccactttggc gccggctcga gtggctccgg tgcccgtcag tgggcagagc
     1921 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     1981 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2041 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2101 acgggtttgc cgccagaaca caggtgtcgt gacgcgggat ccgccaccat gagctcagag
     2161 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
     2221 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
     2281 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
     2341 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
     2401 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
     2461 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
     2521 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
     2581 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
     2641 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
     2701 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
     2761 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
     2821 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa
     2881 agtgacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc
     2941 attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc
     3001 cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa
     3061 gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc
     3121 tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg
     3181 ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc
     3241 aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag
     3301 aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat
     3361 atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat
     3421 gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg
     3481 atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg
     3541 cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat
     3601 cttatcgccc tgtccctcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa
     3661 gataccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc
     3721 cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt
     3781 ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt
     3841 atgatcaagc tctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga
     3901 cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc
     3961 ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg
     4021 gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc
     4081 aaacagcgca ctttcgacaa tggaatcatc ccccaccaga ttcacctggg cgaactgcac
     4141 gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt
     4201 gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc
     4261 agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgagaaa
     4321 gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa
     4381 aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt
     4441 tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg
     4501 tctggagatc agaagaaagc tattgtggac ctcctcttca agacgaaccg gaaagttacc
     4561 gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc
     4621 agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc
     4681 attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc
     4741 ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct
     4801 catctcttcg acgacaaagt catgaagcag ctcaagaggc gccgatatac aggatggggg
     4861 cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg
     4921 gattttctta agtccgatgg atttgccaac cggaacttca ttcagttgat ccatgatgac
     4981 tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt
     5041 cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc
     5101 gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt
     5161 atcgagatgg cccgagagaa ccaaaccacc cagaagggac agaagaacag tagggaaagg
     5221 atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca
     5281 gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg
     5341 gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat
     5401 atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc
     5461 gataaaaaca gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa
     5521 aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg
     5581 actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggtttcat caaaaggcag
     5641 cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac
     5701 accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct
     5761 aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat
     5821 taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa
     5881 tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa
     5941 atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc
     6001 aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga
     6061 ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc
     6121 gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta
     6181 cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc
     6241 gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct
     6301 tacagtgtac tggttgtggc taaagtggag aaagggaagt ctaaaaaact caaaagcgtc
     6361 aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac
     6421 tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag
     6481 tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgtgctg
     6541 cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc
     6601 cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa
     6661 caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg
     6721 atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag
     6781 cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg
     6841 cctgcagcct tcaagtactt cgacactacc atagacagaa agcggtacac ctctacaaag
     6901 gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc
     6961 gacctctctc agctcggtgg agactctggt ggttctacta atctgtcaga tattattgaa
     7021 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
     7081 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
     7141 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
     7201 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
     7261 aagaagaaga ggaaagtcgc tagcggcagc ggcgccacca acttcagcct gctgaagcag
     7321 gccggcgacg tggaggagaa ccccggcccc atgaccgagt acaagcccac ggtgcgcctc
     7381 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7441 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     7501 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7561 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7621 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     7681 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc
     7741 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7801 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7861 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7921 tggtgcatga cccgcaagcc cggtgcctga acgcgttaag tcgacaatca acctctggat
     7981 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8041 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8101 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8161 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8221 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8281 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8341 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8401 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8461 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8521 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     8581 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8641 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8701 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8761 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8821 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8881 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9061 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9121 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9241 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9301 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9361 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9421 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9481 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9541 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9601 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9661 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9721 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9781 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9841 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9901 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9961 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10021 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10081 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10141 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10201 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10261 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10321 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10381 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10441 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10501 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10561 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10621 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10681 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10741 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10801 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10861 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11761 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11821 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11881 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11941 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12001 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12061 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgca
//
