LOCUS       sequence-211526-        9987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Woodsmith J, Apelt L, Casado-Medrano V, Ozkan Z, Timmermann B, 
            Stelzl U
  TITLE     Protein interaction perturbation profiling at amino-acid resolution.
  JOURNAL   Nat Methods. 2017 Dec;14(12):1213-1221. doi: 10.1038/nmeth.4464. 
            Epub 2017 Oct 16.
  PUBMED    29039417
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4464"; journalName: "Nat Methods"; date: "2017-12"; 
            volume: "14"; issue: "12"; pages: "1213-1221"
COMMENT     Sequence Label: pCBDU-JW
FEATURES             Location/Qualifiers
     source          1..9987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      32..1373
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(994..1041)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1638..1742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1743..1811,1812..2603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1961..1980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2774..3362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3263..3282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3516..3533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3548..3688
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3614..3633)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3774..3796
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        3825..4226
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     protein_bind    4269..4393
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4822..5127)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4924..4943)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5447..6130)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     6045..6064
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6131..6233)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(6411..6535)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6546..6566
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6582..6923
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     terminator      7302..7489
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7357..7381)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(7510..7543)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(8042..9136)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9137..9542)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga
       61 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
      121 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
      181 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
      241 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
      301 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
      361 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
      421 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
      481 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
      541 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
      601 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
      661 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
      721 aatagttctt actacaattt tttgtctaaa gagtaatact agagataaac ataaaaaatg
      781 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg
      841 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta
      901 ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt
      961 cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag
     1021 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg
     1081 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat
     1141 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg
     1201 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat
     1261 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc
     1321 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatatgca
     1381 tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg atgagtatac
     1441 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac
     1501 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt
     1561 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc tcaggtggca
     1621 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1681 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1741 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1801 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1861 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1921 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1981 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2041 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2101 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2161 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2221 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2281 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2341 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2401 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2461 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2521 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2581 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2641 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2701 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2761 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2821 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2881 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2941 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3001 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3061 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3121 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3181 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3241 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3301 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3361 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3421 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3481 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3541 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     3601 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     3661 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3721 ctgacgggct tgtctgctcc cgggatccgc ttacagacaa gctgtgaccg tctccgggag
     3781 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc aacttctttt
     3841 cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa tgacaaaaaa
     3901 atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg
     3961 ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc ttcattcacg
     4021 cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt gaatttgaaa
     4081 taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt
     4141 gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc
     4201 aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa gcggaattaa
     4261 ttccggagac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     4321 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     4381 tccagtcact atgaatcaac tacttagatg gtattagtga cctgtagtcg accgacagcc
     4441 ttccaaatgt tcttcgggtg atgctgccaa cttagtcgac cgacagcctt ccaaatgttc
     4501 ttctcaaacg gaatcgtcgt atccagccta ctcgctattg tcctcaatgc cgtattaaat
     4561 cataaaaaga aataagaaaa agaggtgcga gcctcttttt tgtgtgacaa aataaaaaca
     4621 tctacctatt catatacgct agtgtcatag tcctgaaaat catctgcatc aagaacaatt
     4681 tcacaactct tatacttttc tcttacaagt cgttcggctt catctggatt ttcagcctct
     4741 atacttacta aacgtgataa agtttctgta atttctactg tatcgacctg cagactggct
     4801 gtgtataagg gagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     4861 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact
     4921 ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag
     4981 ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg
     5041 cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac ggctctctct
     5101 tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtca gcaaaagagc
     5161 cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc
     5221 gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga ccggagatat
     5281 tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca ctgtaatacg
     5341 ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt atatattctt
     5401 ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag taagccggat
     5461 ccacgcggcg tttacgcccc ccctgccact catcgcagta ctgttgtaat tcattaagca
     5521 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     5581 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     5641 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     5701 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     5761 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     5821 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     5881 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     5941 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     6001 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     6061 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     6121 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     6181 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     6241 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     6301 attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg
     6361 ctgccaactt agtcgactac aggtcactaa taccatctaa gtagttgatt catagtgact
     6421 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     6481 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtaccgg
     6541 agcctccaaa aaagaagaga aaggtcgaat tgggtaccgc cgccaatttt aatcaaagtg
     6601 ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc
     6661 tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc
     6721 atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa
     6781 aaccactgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag
     6841 ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat gatgaagata
     6901 ccccaccaaa cccaaaaaaa gagatctatg aatcgtagat actgaaaaac cccgcaagtt
     6961 cacttcaact gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt
     7021 cttttatgta actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag
     7081 aattttttaa atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa
     7141 aatatattac gagggcttat tcagatgctt tggacttctt cgccagaggt ttggtcaagt
     7201 ctccaatcaa ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg
     7261 gtcaaatcgt tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta
     7321 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     7381 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     7441 tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccggtcg
     7501 agggacctaa taacttcgta tagcatacat tatacgaagt tatattaagg gttccggatc
     7561 gcggcctcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
     7621 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
     7681 attgagtgtt ttttatttgt tgtatttttt tttttagaga aaatcctcca atatataaat
     7741 taggaatcat agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
     7801 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
     7861 aatttgctta cctgtattcc tttacatcct cctttttctc cttcttgata aatgtatgta
     7921 gattgcgtat atagtttcgt ctaccctatg aacatattcc attttgtaat ttcgtgtcgt
     7981 ttctattatg aatttcattt ataaagttta tgtacaaata tcataaaaaa agagaatctt
     8041 tttaagcaag gattttctta acttcttcgg cgacagcatc accgacttcg gtggtactgt
     8101 tggaaccacc taaatcacca gttctgatac ctgcatccaa aaccttttta actgcatctt
     8161 caatggcctt accttcttca ggcaagttca atgacaattt caacatcatt gcagcagaca
     8221 agatagtggc gatagggttg accttattct ttggcaaatc tggagcagaa ccgtggcatg
     8281 gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga ggccaaggac gcagatggca
     8341 acaaacccaa ggaacctggg ataacggagg cttcatcgga gatgatatca ccaaacatgt
     8401 tgctggtgat tataatacca tttaggtggg ttgggttctt aactaggatc atggcggcag
     8461 aatcaatcaa ttgatgttga accttcaatg taggaaattc gttcttgatg gtttcctcca
     8521 cagtttttct ccataatctt gaagaggcca aaacattagc tttatccaag gaccaaatag
     8581 gcaatggtgg ctcatgttgt agggccatga aagcggccat tcttgtgatt ctttgcactt
     8641 ctggaacggt gtattgttca ctatcccaag cgacaccatc accatcgtct tcctttctct
     8701 taccaaagta aatacctccc actaattctc tgacaacaac gaagtcagta cctttagcaa
     8761 attgtggctt gattggagat aagtctaaaa gagagtcgga tgcaaagtta catggtctta
     8821 agttggcgta caattgaagt tctttacgga tttttagtaa accttgttca ggtctaacac
     8881 tacctgtacc ccatttagga ccacccacag cacctaacaa aacggcatca gccttcttgg
     8941 aggcttccag cgcctcatct ggaagtggaa cacctgtagc atcgatagca gcaccaccaa
     9001 ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac atcagaaata gctttaagaa
     9061 ccttaatggc ttcggctgtg atttcttgac caacgtggtc acctggcaaa acgacgatct
     9121 tcttaggggc agacattaga atggtatatc cttgaaatat atatatatat tgctgaaatg
     9181 taaaaggtaa gaaaagttag aaagtaagac gattgctaac cacctattgg aaaaaacaat
     9241 aggtccttaa ataatattgt caacttcaag tattgtgatg caagcattta gtcatgaacg
     9301 cttctctatt ctatatgaaa agccggttcc ggcgctctca cctttccttt ttctcccaat
     9361 ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag
     9421 tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa
     9481 aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag
     9541 ttccttacca ctcttttgtt actctattga tccagctcag caaaggcagt gtgatctaag
     9601 attctatctt cgcgatgtag taaaactagc tagaccgaga aagagactag aaatgcaaaa
     9661 ggcacttcta caatggctgc catcattatt atccgatgtg acgctgcagc ttctcaatga
     9721 tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt tacagattta
     9781 cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag ttttccctga
     9841 aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta cggaagacaa
     9901 tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa tcttgcacgt
     9961 cgcatccccg gttcattttc tgcgttt
//
