LOCUS       sequence-211533-        8970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-regulated miR-E (miR-30 variant)-based RNAi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Fellmann C, Hoffmann T, Sridhar V, Hopfgartner B, Muhar M, Roth M, 
            Lai DY, Barbosa IA, Kwon JS, Guan Y, Sinha N, Zuber J
  TITLE     An optimized microRNA backbone for effective single-copy RNAi.
  JOURNAL   Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 
            10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.
  PUBMED    24332856
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.11.020"; journalName: "Cell Rep"; date: 
            "2013-12-26- 26"; volume: "5"; issue: "6"; pages: "1704-13"
COMMENT     Sequence Label: LT3GEN
FEATURES             Location/Qualifiers
     source          1..8970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             65..109
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    376..493
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        509..887
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    517..535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    553..571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    589..607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    625..643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    661..679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    697..715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    733..751
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     796..820
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(911..913,914..916,917..1630)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(956..977)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1217..1236)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1564..1585
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1904..2403
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1939..1962)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2336..2355
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2417..3220
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2480..2499)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3090..3109
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3268..3856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3321..3341)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3739..3750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4063..4296
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4368..4489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4405..4424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4459..4478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4529..4664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4684..4703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4685..4703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4713..4730)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4713..4729)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4744)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4871..5326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4958..4977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5168..5189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5352..5456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5457..5525,5526..6317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5675..5694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6488..7076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6977..6996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7230..7247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7364..7385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7400..7405,7406..7423,7424..7430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7438..7454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7443..7465
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7462..7478
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7462..7478
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7497..7517
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7499..7517
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7545..7771
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7772..7952
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7999..8124
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8617..8850
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
       61 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
      121 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
      181 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
      241 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
      301 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
      361 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      421 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      481 aattcaaaat tttatcgatt ctagattcga gtttactccc tatcagtgat agagaacgta
      541 tgaagagttt actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg
      601 atagagaacg tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac
      661 tccctatcag tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta
      721 tatccagttt actccctatc agtgatagag aacgtataag ctttaggcgt gtacggtggg
      781 cgcctataaa agcagagctc gtttagtgaa ccgtcagatc gcctggagca attccacaac
      841 acttttgtct tataccaact ttccgtacca cttcctaccc tcgtaaagtc gagcttgcgt
      901 tggatccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      961 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga
     1021 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1081 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1141 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1201 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1261 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1321 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     1381 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt
     1441 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1501 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1561 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1621 gtacaagtaa tgacgtacgg cggccgcctc gactagggat aacagggtaa ttgtttgaat
     1681 gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta
     1741 cttcgacttc ttaacccaac agaaggctcg agcaaccaga attcgagcaa ttatcttgtt
     1801 tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg
     1861 gtataaatta aatcactttt ttcaattgac gcgtaattct accgggtagg ggaggcgctt
     1921 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     1981 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     2041 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     2101 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     2161 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     2221 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     2281 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     2341 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     2401 tcgacctgca gccaatatgg gatcggccat tgaacaagat ggattgcacg caggttctcc
     2461 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     2521 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     2581 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     2641 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     2701 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     2761 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     2821 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     2881 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     2941 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     3001 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3061 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3121 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3181 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatccgt cgacctgcag
     3241 ccaagcttat cgaaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     3301 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3361 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3421 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3481 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3541 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3601 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3661 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3721 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3781 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3841 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     3901 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     3961 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4021 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4081 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4141 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4201 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4261 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4321 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4381 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4441 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4501 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4561 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4621 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4681 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4741 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4801 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4861 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4921 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5101 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5161 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5221 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5281 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5341 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5401 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5461 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5521 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5581 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5641 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5701 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5761 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5821 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5881 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5941 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6001 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6061 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6121 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6181 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6241 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6301 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6361 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6421 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6481 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6541 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6601 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6661 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6721 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6781 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6841 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6901 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6961 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7021 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7081 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7141 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7201 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7261 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7321 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7381 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7441 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7501 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7561 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7621 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7681 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7741 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7801 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7861 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7921 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7981 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8041 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8101 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8161 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8221 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8281 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8341 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8401 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8461 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8521 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8581 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8641 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8701 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8761 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8821 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8881 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8941 gaatcacacg acctggatgg agtgggacag
//
