LOCUS       sequence-211573-        7655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Wagner I, Wang H, Weissert PM, Straube WL, Shevchenko A, Gentzel M, 
            Brito G, Tazaki A, Oliveira C, Sugiura T, Shevchenko A, Simon A, 
            Drechsel DN, Tanaka EM
  TITLE     Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of 
            Dedifferentiating Muscle Cells during Newt Limb Regeneration.
  JOURNAL   Dev Cell. 2017 Mar 27;40(6):608-617.e6. doi: 
            10.1016/j.devcel.2017.03.002.
  PUBMED    28350991
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.03"; journalName: "Dev Cell"; date: 
            "2017-03-27- 27"; volume: "40"; issue: "6"; pages: "608-617.e6"
COMMENT     Sequence Label: pOEM1_pCMV:deltaN-bmp4-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     691..708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1060..1432
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1533..1836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1837..2040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1991..2011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3352..3372)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3770..3781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4016..4150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4053..4072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4107..4126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4263..4295)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4491..4550)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4613..4704)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4671..4688)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4790..5395
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(5433..5557)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(6167..6186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6168..6186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6197..6214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6197..6213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6206..6228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6529..6633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6634..6702,6703..7494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6852..6871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcattaggc
      661 accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga agcggagagc
      721 gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga caggtttccc
      781 gactggaaag cgggcagtga gcggaaggcc catgaggccc aaaaagctca actacaaatt
      841 tgatcgcgga cgtgttagcc gacacaatta ataggcgtcg tgtggctatg gcaaaatcgt
      901 cttcggaagc aacttctaac gacgagggtt gggacgacga cgataatcgg cctaataaag
      961 ctaacacgcc cgatgttaaa tatgtccaag cgttatttaa cgtgtttacg tcgagtcaat
     1021 tgtacactaa cgacagtgat gaaagaaata caaaagcgca taatattttg aacgacgtcg
     1081 aacctttatt acaaaacaaa acacaaacga atatcgacaa agctagattg ctgctacaag
     1141 atttggcaag ttttgtggcg ttgagcgaaa atccattaga tagtccagcc atcggttcgg
     1201 aaaaacaacc cttgtttgaa actaatcgaa acctatttta caaatctatt gaggatttaa
     1261 tatttaaatt cagatataaa gacgctgaaa atcatttgat tttcgctcta acataccacc
     1321 ctaaagatta taaatttaat gaattattaa aatacgtaca acaattgtct gtaaatcaac
     1381 aacgcacaga atctagcgct taataaatgt actaataaca atgtatcgtg ttttattaat
     1441 taagatctgc ggatgcaggc tccgccatgc attagttatt aatagtaatc aattacgggg
     1501 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1561 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1621 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1681 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1741 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1801 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1861 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1921 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1981 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2041 ggtttagtga accgtcagat ccgctagcgc taccggtgcc gccaccatga ttcctggtaa
     2101 ccgaatgctg atggtcgttt tattatgcca agtcctgcta ggaggcgcga gccatgctag
     2161 tttgatacct gagacgggga agaaaaaagt cgccgagatt cagggccacg cgggaggacg
     2221 ccgctcaggg cagagccatg agctcctgcg ggacttcgag gcgacacttc tgcagatgtt
     2281 tgggctgcgc cgccgcccgc agcctagcaa gagtgccgtc attccggact acatgcggga
     2341 tctttaccgg cttcagtctg gggaggagga ggaagagcag atccacagca ctggtcttga
     2401 gtatcctgag cgcccggcca gccgggccaa caccgtgagg agcttccacc acgaagaaca
     2461 tctggagaac atcccaggga ccagtgaaaa ctctgctttt cgtttcctct ttaacctcag
     2521 cagcatccct gagaacgagg cgatctcctc tgcagagctt cggctcttcc gggagcaggt
     2581 ggaccagggc cctgattggg aaaggggctt ccaccgtata aacatttatg aggttatgaa
     2641 gcccccagca gaagtggtgc ctgggcacct catcacacga ctactggaca cgagactggt
     2701 ccaccacaat gtgacacggt gggaaacttt tgatgtgagc cctgcggtcc ttcgctggac
     2761 ccgggagaag cagccaaact atgggctagc cattgaggtg actcacctcc atcagactcg
     2821 gacccaccag ggccagcatg tcaggattag ccgatcgtta cctcaaggga gtgggaattg
     2881 ggcccagctc cggcccctcc tggtcacctt tggccatgat ggccggggcc atgccttgac
     2941 ccgacgccgg agggccaagc gtagccctaa ctgccggcgc cactcgctct atgtggactt
     3001 cagcgatgtg ggctggaatg actggattgt ggccccacca ggctaccagg ccttctactg
     3061 ccatggggac tgcccctttc cactggctga ccacctcaac tcaaccaacc atgccattgt
     3121 gcagaccctg gtcaattctg tcaattccag tatccccaaa gcctgttgtg tgcccactga
     3181 actgagtgcc atctccatgc tgtacctgga tgagtatgat aaggtggtac tgaaaaatta
     3241 tcaggagatg gtagtagagg gatgtgggtg ccgctgagcg gccgctagac gcgtcgacaa
     3301 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3361 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3421 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3481 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3541 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3601 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3661 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     3721 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3781 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3841 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgaagc ttgtcgagaa
     3901 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3961 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     4021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4141 tgtctggatc tgatcactgg cgcgccgtga tgcgtatttc tgaatctttg taaaatagca
     4201 cacaagactc caacgcgttt ggcgttttat tttcttgctc gactctagtt tattaggcct
     4261 tactttccaa gtcggttcat ctctatgtct gtataaatct gtcttttctt ggtgtgcttt
     4321 aatttaatgc aaagatggat accaactcgg agaaccaaga atagtccaat gattaaccct
     4381 atgataaaga aaaaagaggc aatagagctt ttccaactac tgaaccaacc ttctacaagc
     4441 tcgattggat ttttggatag cccagtatca ccccggggta cctgcagatc cgcaaaggca
     4501 gaatgcgccg ccgccgccaa aagcacatat aaaacaatag cgcttaccat cttgcttgtg
     4561 tgttccttat tgaagccttg gtgtgactga tttactagta gcatagatcc gtatttatag
     4621 gtttttttat tacaaaactg ttacgaaaac agtaaaatac ttatttattt gcgagatggt
     4681 tatcatttta attatctcca tgatccagcc aaccactaga actatagcta gagtcctggg
     4741 cgaacaaaat ggtggcagct tgtgatatta gtttgtgcgt ctcattacaa tggctgttat
     4801 ttttaacaac aaacaactgc tcgcagacaa tagtatagaa aagggaggtg aactgttttt
     4861 gtttaacggt tcgtacaaca ttttggaaag ttatgttaat ccggtgctgc taaaaaatgg
     4921 tgtaattgaa ctagaagaag ctgcgtacta tgccggcaac atattgtaca aaaccgacga
     4981 tcccaaattc attgattata taaatttaat aattaaagca acacactccg aagaactacc
     5041 agaaaatagc actgttgtaa attacagaaa aactatgcgc agcggtacta tacaccccat
     5101 taaaaaagac atatatattt atgacaacaa aaaatttact ctatacgata gatacatata
     5161 tggatacgat aataactatg ttaattttta tgaggagaaa aatgaaaaag agaaggaata
     5221 cgaagaagaa gacgacaagg cgtctagttt atgtgaaaat aaaattatat tgtcgcaaat
     5281 taactgtgaa tcatttgaaa atgattttaa atattacctc agcgattata actacgcgtt
     5341 ttcaattata gataatacta caaatgttct tgttgcgttt ggtttgtatc gttaataaaa
     5401 aacaaattta gcatttataa ttgttttatt attcaataat tacaaatagg attgagaccc
     5461 ttgcagttgc cagcaaacgg acagagcttg tccacagttt tcattttttg gcagtttggt
     5521 ggtttataca catcttggga tttgattaaa agatgcatga cttcacccac acacttggag
     5581 tcgtggttgt agaaatgcgt cagagccttg attaaacatc ggttttcaca cttggcacac
     5641 gaaaccacgt ttgcaatgta acagtttagt ataaacctct tcctgtacat accgccgcgc
     5701 ggtacattgt tttctttcaa atcgttcaat atttttttta tacacggagg catattaatc
     5761 ttgttgttca gcatgtccac gacatttttc tttccttcta cgcattcttt catacaaatg
     5821 tttctcttgc tgcgtttttt aaaattggaa tttttaattt cattaatagt gggcggagcc
     5881 gttaacagca tgtacaatcg cagtccggaa attttgacca aaaccccgcc attgtagaat
     5941 accgtgtatg gagttagggt caatttatca ataaaatcat cgggatcaat taaatatgta
     6001 gctttcttgt ctaaacatga cgctctaatg agcggactcc acacgttata cgatgcattc
     6061 gccatacttc gcggcttctc gcaccagcgg tcctgattcg actaacggtc gattggatgg
     6121 cttaatagtt ttaacgtcac ggccttgacg gccttccgcc aattcgccct atagtgagtc
     6181 gtattacgtc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6241 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6301 aggcccgcac cgaaacgccc ttcccaacag ttgcgcagcc tgaatggcga atgggagcgc
     6361 cctgtagcgg ccactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6421 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6481 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7321 gctgataaat ctggagccgg tgagcgtggt tctcgcggta tcattgcagc actggggcca
     7381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7621 ttccactgag cgtcagaccc cgtagaaaag atcaa
//
