LOCUS       sequence-211593-        4724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4724)
  TITLE     Unpublished plasmid
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMT-puro
FEATURES             Location/Qualifiers
     source          1..4724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..24
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16..33
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17..33
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        39..465
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             562..603
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             613..630
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(653..670)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    672..806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(696..715)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     750..769
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(928..1527)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1011..1031)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1508..1527
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(2141..2157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2141..2157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2154..2176)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2165..2181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2189..2195,2196..2213,2214..2219))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2234..2255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2372..2389)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2543..3131)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2623..2642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3302..4093,4094..4162))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3925..3944
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4163..4267)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4335..4353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4391..4413)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4513..4532
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcccagtcac gacgttgtaa aacgacggcc agtgaattaa ttcgttgcag gacaggatgt
       61 ggtgcccgat gtgactagct ctttgctgca ggccgtccta tcctctggtt ccgataagag
      121 acccagaact ccggcccccc accgcccacc gccaccccca tacatatgtg gtacgcaagt
      181 aagagtgcct gcgcatgccc catgtgcccc accaagagct ttgcatccca tacaagtccc
      241 caaagtggag aaccgaacca attcttcgcg ggcagaacaa aagcttctgc acacgtctcc
      301 actcgaattt ggagccggcc ggcgtgtgca aaagaggtga atcgaacgaa agacccgtgt
      361 gtaaagccgc gtttccaaaa tgtataaaac cgagagcatc tggccaatgt gcatcagttg
      421 tggtcagcag caaaatcaag tgaatcatct cagtgcaact aaagggggga tctagatcgg
      481 ggtacctact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
      541 gagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
      601 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
      661 gtgccttcta agatccagac atgataagat acattgatga gtttggacaa accacaacta
      721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      781 ccattataag ctgcaataaa caagttctag agtcggtggg cctcgggggc gggtgcgggg
      841 tcggcggggc cgccccgggt ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt
      901 cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt
      961 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     1021 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     1081 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     1141 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     1201 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     1261 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     1321 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     1381 cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta
     1441 gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag
     1501 gcgcaccgtg ggcttgtact cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg
     1561 tgggtcaggg gatggtggcg gcaccggtcg tggcggccga cctgcaggca tgcaagctat
     1621 cgaattcctg cagcccgggg gatctgttgt aatttataat ttatatttcc tttcttaata
     1681 aataaataaa tagtcaagtt tatgtttgag ttttatgatt tatattttaa gttatttcaa
     1741 ctgcaacacc agcaccacga cctacttaca gcaaaaaacg tacaagaagg aaagaaggaa
     1801 taaaaagagt ggtattctct tacaatatgt tttatggcat aaaaggtgtg gccattcata
     1861 tcaaatataa agtagtgttg tttaacgtta cttttgtagg ttgaatagta tattccaaca
     1921 gatgatgagg ggttcccaat cctaaaccca tttgccgttc ccagaagcat gaaaccacca
     1981 cgcaccggat cctctagaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     2041 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     2101 atcagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2161 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2221 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2281 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2341 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2401 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2461 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2521 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2581 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2641 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2701 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2761 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2821 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2881 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2941 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3001 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3061 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3121 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3181 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3241 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3301 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3361 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3421 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3481 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3541 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3601 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3661 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3721 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3781 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3841 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3901 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3961 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4021 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4081 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4141 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4201 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4261 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4321 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4381 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4441 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4501 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4561 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     4621 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     4681 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttt
//
