LOCUS       sequence-211607-       13569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA 
            expression cassette, a T2A-puro selection cassette, and two gRNA 
            recognition sequences at both ends of the whole insertion DNA 
            fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   Ma S, Lv J, Sun J, Tang P, Li H, Zhou H, Zhang Z, Lin Y, Rong Z
  TITLE     iKA-CRISPR hESCs for inducible and multiplex orthogonal gene 
            knockout and activation.
  JOURNAL   FEBS Lett. 2018 Jun 5. doi: 10.1002/1873-3468.13127.
  PUBMED    29869798
REFERENCE   2  (bases 1 to 13569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2018 Jun 5. doi: 10.1002/1873-3468.13127."
COMMENT     Sequence Label: Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1
FEATURES             Location/Qualifiers
     source          1..13569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    706..731
                     /label=SA
                     /note="splice acceptor site"
     CDS             755..808
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             818..1417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(818..837)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1314..1334
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1449..1466)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1455..1679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    1686..1767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1711..1730
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(1782..1790,1791..1826,1827..1829,1830..1865,
                     1866..1868,1869..1904,1905..1910,1911..2528))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2601..2620)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2628..3645
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2682..2704)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3647..3924)
                     /label=chicken beta-actin promoter
     enhancer        3926..4229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    4327..4788
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(4813..6663)
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5040..5049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6667..6687)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6700..10803)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6700..10803)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6700..10803)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(10825..10845)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10852..10917)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(10954..10978)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(10995..11309)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(10998..11022)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11069..11087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11105..11123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11140..11158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11176..11194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11212..11230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11247..11265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11283..11301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(11384..11402)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11423..11439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11423..11439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11436..11458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11447..11463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11471..11477,11478..11495,11496..11501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11516..11537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11654..11671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11825..12413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11905..11924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12584..13375,13376..13444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13207..13226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13445..13549)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      661 cgggcccccc ctcgaggtca ccaatcctgt ccctagtgga agcttctgac ctcttctctt
      721 cctcccacag ggcctcgaga gatctggcag cggagagggc agaggaagtc ttctaacatg
      781 cggtgacgtg gaggagaatc ccggccctag gctcgagatg accgagtaca agcccacggt
      841 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
      901 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
      961 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     1021 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     1081 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     1141 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     1201 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     1261 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     1321 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     1381 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct agagggcccg tttaaacccg
     1441 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1501 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1561 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     1621 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg
     1681 tcgaggcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     1741 accattataa gctgcaataa acaagttaac aacaacaatt gttacccggg gagcatgtca
     1801 aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca
     1861 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggccggccc gccgctttcg
     1921 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt
     1981 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg
     2041 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag
     2101 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct
     2161 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg
     2221 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gaatgacttg
     2281 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga
     2341 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc
     2401 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct
     2461 tcgataccga ctccattgag taattccaga gcgccgttta tgactttgct cttgtccagt
     2521 ctagacatgg tgaattcctg cagcccgggg gatccactag cttcgatcaa gccgaattct
     2581 ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg taggaaaaag
     2641 aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg ccagaagccg
     2701 aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca gccccgaggc
     2761 tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc gcccatttcc
     2821 ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg gcggcgcctc
     2881 ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct ctcgcacgat
     2941 taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc gctccggggg
     3001 ccgccgcgcc cctcccccga gccctccccg gcccgaggcg gccccgcccc gcccggcacc
     3061 cccacctgcc gccacccccc gcccggcacg gcgagccccg cgccacgccc cgtacggagc
     3121 cccgcacccg aagccgggcc gtgctcagca actcggggag gggggtgcag ggggggttgc
     3181 agcccgaccg acgcgcccac accccctgct caccccccca cgcacacacc ccgcacgcag
     3241 cctttgttcc cctcgcagcc ccccccgcac cgcggggcac cgcccccggc cgcgctcccc
     3301 tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc acagccgccg
     3361 ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac acacgcacgc accccccgag
     3421 ccgctccccc cgcacaaagg gccctcccgg agcccctcaa ggctttcacg cagccacaga
     3481 aaagaaacaa gccgtcatta aaccaagcgc taattacagc ccggaggaga agggccgtcc
     3541 cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg
     3601 gcggcggagc ggggcacggg gcgaaggcag cgtcgcagcg actccccgcc cgccgcgcgc
     3661 ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg
     3721 cgccgctctg attggctgcc gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc
     3781 gccccgcccc gcctggcgcg cgcccccccc ccccccccgc ccccatcgct gcacaaaata
     3841 attaaaaaat aaataaatac aaaattgggg gtggggaggg gggggagatg gggagagtga
     3901 agcagaacgt ggggctcacc tcgaccatgg taatagcgat gactaatacg tagatgtact
     3961 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4021 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4081 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4141 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     4201 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     4261 accccgtaat tgattactat taataactag ttctagagcg gccgccaccg cggtggagct
     4321 cccagcttct gatggaatta gaacttggca aaacaatact gagaatgaag tgtatgtgga
     4381 acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga aaccacagta
     4441 cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag atctatagat
     4501 ctatagatca tgagtgggag gaatgagctg gcccttaatt tggttttgct tgtttaaatt
     4561 atgatatcca actatgaaac attatcataa agcaatagta aagagccttc agtaaagagc
     4621 aggcatttat ctaatcccac cccaccccca cccccgtagc tccaatcctt ccattcaaaa
     4681 tgtaggtact ctgttctcac ccttcttaac aaagtatgac aggaaaaact tccattttag
     4741 tggacatctt tattgtttaa tagatcatca atttctgcag acttacagcg gatccccacg
     4801 cgtttaaccg gtgtcctggc tctgcgtgtg cagctccacc agcatgcaca tggtggacca
     4861 ctgggctctt cggagtgaag agaactccag gtgcttgtcc cttgccagcg tgagaaacgc
     4921 atcccgacca tccatcagat cgcaggggat gagaggatca ggatcaacaa tgggaggcag
     4981 ggagttggca gcagggccag caatgaggcg gatcacaaag aagacctctt tatgcttctc
     5041 catggtggca tatagtttct gtgagaggtc gttagataca ttgggcatcc cgggtttctt
     5101 cttgttgccc ctactcaggc tgctcttatt tttgctggtt ttcttattat tcttcttttt
     5161 agcatttttg ctgtctccct tggtcacatc tgtgctttca ttgctggtgt tttcctctcg
     5221 ttttctctct tcttcctcct gttccagttc cttaatgctt tcttccagaa cattgggcca
     5281 gaaatcaccc tcgaaataag gcaattcctt tgcacttgtt aatctatctt cagtagcttg
     5341 tttaaaaata tccttgtagt catggacaat acgctctgat acagccttgt caagcatttt
     5401 tttgtaccat tcctgcagtc gcttgggctt gggtatcttc tggtcaggag gatggcaatg
     5461 gaagatataa tcatctccct cacttggtgg acatgcccaa atatgccctg ttgtgtaacc
     5521 taatttcttg acatattcta aatatccaat taggatttca tgatagactg cagtcctcaa
     5581 gcatttagga cggaagaaat gaacactatc gaggtaagat atgtatactc tcctctggtt
     5641 gggtggaggg cagtcagagc catactcttg aacatgcatg ccaaagaagc acaggtcaac
     5701 accatcaatt tcttcaaagg caaagagggc tttggttcgg tatggaaagg attctgccat
     5761 ctctccactg tccacaaacc ttgctttcat gcctggtttt acttccacgg ttttgtcaga
     5821 agcatgaact actctaacag tgacctctcc tgactcaggg tgattctgtc gcctcagaaa
     5881 gtcattcaca cgattctcta gaaaggtgcc aagtctggta gatggcaacc ttttagcaga
     5941 aaacttattt tctttcctag ttcgtgcact tttctttaaa cagccatcac agacgaatcc
     6001 agcaggccag atgatctcat ggtgaaggac acagatctga tgcatctttc ttccgcactc
     6061 tgtacattca acaaacagtt caggatccag tgtgtcattt tttctcttgg aaaattgttc
     6121 tttatttatt gtagtttgag gctgggaagg gtcatccccc aaagaaacgc tctccccttg
     6181 gatctcattg aaacacttct cacagaaatg atacctgttc tggtaactgt aataagtggc
     6241 atcacgaggt attgtgcaca actgtttgcc gtagcaacac agtgtctgtg gagagaactc
     6301 caactttctg ccgcaacaat atccaaggct ttgcatcact gggtcaattt cttgttcaaa
     6361 gacctcagag agcttggagc agtatttgta tacccgtgat gttttccggt tatataacca
     6421 ggcattattg aacataagcc aaatatcatc gacatactgc cagggctcct gatactgtcc
     6481 agtgtctaac ttcctcttaa tggtagaaag atccatgggg ctcttcacaa tatcaaagta
     6541 atcagggatt cctaaaagct gagggtccac aggttgacga aagggaaggg attctggatc
     6601 ctgacggtaa agtgcctcca aagttggcat cagtgcctgt cgtagttctt ctggtttgaa
     6661 aatgctcacc ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag
     6721 gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac
     6781 ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc
     6841 aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat
     6901 gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag
     6961 gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg
     7021 ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata
     7081 gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt
     7141 ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct caaagagaga
     7201 gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt tcgcctcgag
     7261 aaagtcgatg gggttttttt cgaagcttga tcgctccatg attgtgatgc ccagcagttc
     7321 cttgacgctt ttgagttttt tagacttccc tttctccact ttggccacaa ccagtacact
     7381 gtaagcgact gtaggagaat cgaatccgcc gtatttcttg gggtcccaat cttttttgcg
     7441 tgcgatcagc ttgtcgctgt tccttttcgg gaggatactt tccttggaga agcctccggt
     7501 ctgtacttcg gtctttttaa cgatgttcac ctgcggcatg gacaggacct tccggactgt
     7561 cgcgaaatcc ctacccttgt cccacacgat ttctcctgtt tctccgtttg tttcgataag
     7621 tggtcgcttc cgaatctctc cattggccag tgtaatctcg gtcttgaaaa aattcataat
     7681 attgctgtaa aagaagtact tagcggtggc cttgcctatt tcctgctcag actttgcgat
     7741 cattttccta acatcgtaca ctttatagtc tccgtaaaca aattcagatt caagcttggg
     7801 atattttttg ataagtgcag tgcctaccac tgcattcagg taggcatcat gcgcatggtg
     7861 gtaattgttg atctctctca ccttataaaa ctgaaagtcc tttctgaaat ctgagaccag
     7921 cttagacttc agagtaataa ctttcacctc tcgaatcagt ttgtcatttt catcgtactt
     7981 ggtgttcatg cgtgaatcga gaatttgggc cacgtgcttg gtgatctggc gtgtctcaac
     8041 aagctgcctt ttgatgaagc cggctttatc caactcagac aggccacctc gttcagcctt
     8101 agtcagatta tcgaacttcc gttgtgtgat cagtttggcg ttcagcagct gccgccaata
     8161 atttttcatt ttcttgacaa cttcttctga ggggacgtta tcactcttcc ctctattttt
     8221 atcggatctt gtcaacactt tattatcaat agaatcatct ttgagaaaag actggggcac
     8281 gatatgatcc acgtcgtagt cggagagccg attgatgtcc agttcctgat ccacgtacat
     8341 gtccctgccg ttctgcaggt agtacaggta gagcttctca ttctgaagct gggtgttttc
     8401 aactgggtgt tccttaagga tttgggaccc cagttctttt ataccctctt caatcctctt
     8461 catcctttcc ctactgttct tctgtccctt ctgggtagtt tggttctctc gggccatctc
     8521 gataacgata ttctcgggct tatgccttcc cattactttg acgagttcat ccacgacctt
     8581 aacggtctgc agtattccct ttttgatagc tgggctacct gcaagattag cgatgtgctc
     8641 gtgaagactg tccccctggc cagaaacttg tgctttctgg atgtcctcct taaaggtgag
     8701 agagtcatca tggatcaact gcatgaagtt ccggttggca aatccatcgg acttaagaaa
     8761 atccaggatt gtctttccac tctgcttgtc tcggatccca ttgatcagtt ttcttgacag
     8821 ccgcccccat cctgtatatc ggcgcctctt gagctgtttc atgactttgt cgtcgaagag
     8881 atgagcgtaa gttttcaagc gttcttcaat catctcccta tcttcaaaca acgtaagggt
     8941 gaggacaatg tcctcaagaa tgtcctcgtt ctcctcattg tccaggaagt ccttgtcttt
     9001 aatgattttc aggagatcgt gatacgttcc cagggatgcg ttgaagcgat cctccactcc
     9061 gctgatttca acagagtcga aacattcaat ctttttgaaa tagtcttctt tgagctgttt
     9121 cacggtaact ttccggttcg tcttgaagag gaggtccacg atagctttct tctgctctcc
     9181 agacaggaat gctggctttc tcatcccttc tgtgacgtat ttgaccttgg tgagctcgtt
     9241 ataaactgtg aagtactcgt acagcagaga gtgtttagga agcacctttt cgttaggcag
     9301 atttttatca aagttagtca tcctttcgat gaaggactgg gcagaggccc ccttatccac
     9361 gacttcctcg aagttccagg gagtgatggt ctcttctgat ttgcgagtca tccacgcgaa
     9421 tctggaattt ccccgggcga gggggcctac atagtagggt atccgaaatg tgaggatttt
     9481 ctcaatcttt tccctgttat ctttcaaaaa ggggtagaaa tcctcttgcc gcctgaggat
     9541 agcgtgcagt tcgcccaggt gaatctggtg ggggatgctt ccattgtcga aagtgcgctg
     9601 tttgcgcaac agatcttctc tgttaagctt taccagcagc tcctcggtgc cgtccatttt
     9661 ttccaagatg ggcttaataa atttgtaaaa ttcctcctgg cttgctccgc cgtcaatgta
     9721 tccggcgtag ccatttttag actgatcgaa gaaaatttcc ttgtacttct caggcagttg
     9781 ctgtctgaca agggccttca gcaaagtcaa gtcttggtgg tgctcatcat agcgcttgat
     9841 catactagcg ctcagcggag ctttggtgat ctccgtgttc actcgcagaa tatcactcag
     9901 cagaatggcg tctgacaggt tctttgccgc caaaaaaagg tctgcgtact ggtcgccgat
     9961 ctgggccagc agattgtcga gatcatcatc gtaggtgtct ttgctcagtt gaagcttggc
    10021 atcttcggcc aggtcgaagt tagatttaaa gttgggggtc agcccgagtg acagggcgat
    10081 aagattacca aacaggccgt tcttcttctc cccagggagc tgtgcgatga ggttttcgag
    10141 ccgccgggat ttggacagcc tagcgctcag gattgctttg gcgtcaactc cggatgcgtt
    10201 gatcgggttc tcttcgaaaa gctgattgta agtctgaacc agttggataa agagtttgtc
    10261 gacatcgctg ttgtctgggt tcaggtcccc ctcgatgagg aagtgtcccc gaaatttgat
    10321 catatgcgcc agcgcgagat agatcaaccg caagtcagcc ttatcagtac tgtctacaag
    10381 cttcttcctc agatgatata tggttgggta cttttcatgg tacgccacct cgtccacgat
    10441 attgccaaag attgggtggc gctcgtgctt tttatcctcc tccaccaaaa aggactcctc
    10501 cagcctatgg aagaaagagt catccacctt agccatctca ttactaaaga tctcctgcag
    10561 gtagcagatc cgattctttc tgcgggtata tctgcgccgt gctgttcttt tgagccgcgt
    10621 ggcttcggcc gtctccccgg agtcgaacag gagggcgcca atgaggttct tctttatgct
    10681 gtggcgatcg gtattgccca gaactttgaa ttttttgctc ggcaccttgt actcgtccgt
    10741 aatgacggcc cagccgacgc tgtttgtgcc gatatcgagc ccaatggagt acttcttgtc
    10801 catgggtacc ccgcggtgaa tgcccacctt cctcttcttc ttgggggcca tcttgtcatc
    10861 gtcatccttg taatcgatgt catgatcttt ataatcaccg tcatggtctt tgtagtccat
    10921 ggtgctagct ccggtccaac ctgaaaaccg cggagcctgc ttttttgtac aaacttgttg
    10981 atcggaattc tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc
    11041 caccgtacac gcctacctcg acatacgttc tctatcactg atagggagta aactcgacat
    11101 acgttctcta tcactgatag ggataaactc gacatacgtt ctctatcact gatagggagt
    11161 aaactcgaca tacgttctct atcactgata gggagtaaac tcgacatacg ttctctatca
    11221 ctgataggga gtaaactcga catcgttctc tatcactgat agggagtaaa ctcgacatac
    11281 gttctctatc actgataggg agtaaactcg acagtactaa gcttgtcacc aatcctgtcc
    11341 ctagtgggcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
    11401 tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    11461 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    11521 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    11581 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    11641 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    11701 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    11761 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    11821 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    11881 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    11941 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    12001 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    12061 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    12121 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    12181 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    12241 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
    12301 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    12361 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    12421 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    12481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    12541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    12601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    12661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    12721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    12781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    12841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    12901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    12961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    13021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    13081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    13141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    13201 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    13261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    13321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    13381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    13441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    13501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    13561 gaaaagtgc
//
