LOCUS       sequence-211684-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1162
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        13..392
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     547..567
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             610..790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    837..962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1459..1692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1877..1921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2219..2336
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2597..2630)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2634..2654
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2907..3118
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3119..4057
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4014..4034
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4078..4674
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4078..4097)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4574..4594
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4684..4737
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4744..4753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4750..4752,4753..4755,4756..5469)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4795..4816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5056..5075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5403..5424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5489..5522)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5578..6166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5631..5651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6049..6060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6169..6185)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6170..6186)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6695..6875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6937..7525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7017..7036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7696..8487,8488..8556))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8319..8338
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8557..8661)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8736..8755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
       61 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      121 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      181 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      241 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      301 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      361 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      421 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      481 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      541 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
      601 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      781 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      841 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
      901 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
      961 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1021 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1081 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1141 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     1201 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1261 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     1321 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1381 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1441 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1501 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1561 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1621 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1681 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1741 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1801 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1861 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1921 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1981 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2041 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2101 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2161 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2221 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2281 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2341 tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgccc tgcaggtcta
     2401 gagatccgac gcgccatctc taggcccgcg ccggccccct cgcacggact tgtgggagaa
     2461 gctcggctac tcccctgccc cggttaattt gcatataata tttcctagta actatagagg
     2521 cttaatgtgc gataaaagac agataatctg ttctttttaa tactagctac attttacatg
     2581 ataggcttgg atttctataa cttcgtatag catacattat acgaagttat aaacagcaca
     2641 aaaggaaact caccctaact gtaaagtaat tgtgtgtttt gagactataa gtatcccttg
     2701 gagaaccacc ttgttggttg gcgcctacag ccccgggttt aagagctaag ctggaaacag
     2761 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2821 gctttttttc tcgagtacta ggatccatta ggcggccgcc ctgcagggtg gataaccgta
     2881 ttaccgccat gcatgtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2941 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     3001 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3061 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     3121 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     3181 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     3241 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     3301 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     3361 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     3421 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     3481 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     3541 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     3601 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     3661 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     3721 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     3781 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     3841 cctcgattag ttctcgcgct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     3901 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     3961 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     4021 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcta gcccaccatg
     4081 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     4141 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     4201 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4261 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4321 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4381 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4441 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     4501 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4561 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     4621 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg
     4681 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg
     4741 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4801 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4861 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gccggtgccc
     4921 tggcccaccc tcgtgaccac cctgacccat ggcgtgcagt gcttcagccg ctaccccgac
     4981 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5041 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5101 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     5161 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     5221 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     5281 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5341 gacaaccact acctgagcac ccagtccaag ctgagtaaag accccaacga gaagcgcgat
     5401 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5461 tacaagtaag aattcgtcga gggacctaat aacttcgtat agcatacatt atacgaagtt
     5521 atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct tatcgataat
     5581 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5641 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5701 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5761 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5821 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5881 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5941 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6001 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6061 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6121 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6181 cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6241 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     6301 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6361 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6421 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     6481 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     6541 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     6601 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     6661 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     6721 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6781 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6841 cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa
     6901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7681 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7741 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7801 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7861 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7921 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7981 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8041 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8101 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8161 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8221 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8281 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8341 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8401 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8461 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8521 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8641 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     8701 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8761 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     8821 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     8881 aggcgttttg cgctgcttcg cgatgtacgg gcca
//
