LOCUS       sequence-211964-        4702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length PARP2 in Sf9 (insect) cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Liszczak G, Diehl KL, Dann GP, Muir TW
  TITLE     Acetylation blocks DNA damage-induced chromatin ADP-ribosylation.
  JOURNAL   Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1.
  PUBMED    30013063
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 
            10.1038/s41589-018-0097-1."
COMMENT     Sequence Label: pACEBac1-PARP2-Flag-His-WT
FEATURES             Location/Qualifiers
     source          1..4702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  135..358
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(425..958)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1147..1152,1153..1169,1170..1175))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(1246..1279)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1356..1447
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1372..1389
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             complement(2832..2843)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3254..3277
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3314..3331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3504..3638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3541..3560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3595..3614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3759..3924
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4114..4702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4603..4622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat gcgcagatgc
      121 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
      181 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
      241 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
      301 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga ctatattcgc
      361 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga
      421 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
      481 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
      541 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
      601 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
      661 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
      721 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
      781 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
      841 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
      901 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
      961 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1021 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     1081 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     1141 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     1201 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgccataac ttcgtatagc
     1261 atacattata cgaagttatc tgtaactata acggtcctaa ggtagcgagt ttaaacacta
     1321 gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa
     1381 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     1441 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcca tggcagccag
     1501 aaggaggagg tccacgggcg gaggaagggc ccgcgctttg aatgagagca aaagggtaaa
     1561 caatggcaat accgccccag aggacagctc cccagctaag aaaactagaa gatgtcagcg
     1621 ccaggaatct aaaaagatgc ccgtggctgg aggaaaggca aacaaggacc gtacggaaga
     1681 caagcaagat ggaatgcctg gaaggtcttg ggcaagtaaa agagtttcgg agagcgttaa
     1741 agctctcctg ctcaagggta aagcgccagt ggacccggag tgtaccgcca aggtcggcaa
     1801 ggctcatgtt tactgcgagg gcaacgatgt gtatgacgtg atgctcaatc aaaccaattt
     1861 gcagtttaac aacaacaaat actacctcat ccaacttttg gaggacgatg cacaacgcaa
     1921 cttttcagtg tggatgcgct ggggtcgcgt cggcaagatg ggtcaacact ctctcgttgc
     1981 ttgttcagga aatctcaaca aggcgaaaga aatctttcaa aagaaatttt tggataaaac
     2041 caagaacaat tgggaagata gggaaaagtt cgagaaggtg cccggaaagt atgatatgct
     2101 gcaaatggat tatgcgacaa acactcagga tgaagaagag acaaaaaagg aagaatcgct
     2161 taagtcgcca ctcaaacctg aaagccaact tgatcttcgg gtacaggagt taataaagtt
     2221 gatctgtaat gttcaggcca tggaagaaat gatgatggaa atgaagtata ataccaagaa
     2281 agccccactt gggaagctga cagtggcaca aatcaaggca ggttaccagt ctcttaagaa
     2341 gattgaggat tgtattcggg ctggccagca tggacgagct ctcatggaag catgcaatga
     2401 attctacacc aggattccgc atgactttgg actccgtact cctccactaa tccggacaca
     2461 gaaggaactg tcagaaaaaa tacaattact agaggctttg ggagacattg aaattgctat
     2521 taagctggtg aaaacagagc tacaaagccc agaacaccca ttggaccaac actatagaaa
     2581 cctacattgt gccttgcgcc cccttgacca tgaaagttat gagttcaaag tgatttccca
     2641 gtacctacaa tctacccatg ctcccacaca cagcgactat accatgacct tgctggattt
     2701 gtttgaagtg gagaaggatg gtgagaaaga agccttcaga gaggaccttc ataacaggat
     2761 gcttctatgg catggttcca ggatgagtaa ctgggtggga atcttgagcc atgggcttcg
     2821 aattgcccca cctgaagctc ccatcacagg ttacatgttt gggaaaggaa tctactttgc
     2881 tgacatgtct tccaagagtg ccaattactg ctttgcctct cgcctaaaga atacaggact
     2941 gctgctctta tcagaggtag ctctaggtca gtgtaatgaa ctactagagg ccaatcctaa
     3001 ggccgaagga ttgcttcaag gtaaacatag caccaagggg ctgggcaaga tggctcccag
     3061 ttctgcccac ttcgtcaccc tgaatgggag tacagtgcca ttaggaccag caagtgacac
     3121 aggaattctg aatccagatg gttataccct caactacaat gaatatattg tatataaccc
     3181 caaccaggtc cgtatgcggt accttttaaa ggttcagttt aatttcctcc aactttgggg
     3241 cggatctggc ggtgactata aagatgatga tgataaagga ggctccgtcg acgagctcac
     3301 tggaggtagc ggacaccatc accatcacca ctgatgtcgc ggccgctttc gaatctagag
     3361 cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     3421 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3481 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3601 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa
     3661 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     3721 taactatcat aacccctagg gtatacccat ctaattggaa ccagataagt gaaatctagt
     3781 tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca cccagttccc
     3841 atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac ccctcccagt
     3901 tcccaactat tttgtccgcc cacaaccggt tgacttgggt caactgtcag accaagttta
     3961 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4021 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4681 aggggggcgg agcctatgga aa
//
