LOCUS       sequence-211990-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, 
            Spaink HP
  TITLE     Use of green fluorescent protein color variants expressed on stable 
            broad-host-range vectors to visualize rhizobia interacting with 
            plants.
  JOURNAL   Mol Plant Microbe Interact. 2000 Nov;13(11):1163-9. doi: 
            10.1094/MPMI.2000.13.11.1163.
  PUBMED    11059482
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI.2000.13.11"; journalName: "Mol Plant Microbe 
            Interact"; date: "2000-11"; volume: "13"; issue: "11"; pages: 
            "1163-9"
COMMENT     Sequence Label: pMP4657
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(31..50)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     378..399
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1220..1870)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             1976..3175
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             4381..5010
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5439..6512
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6578..6772
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7481..8026
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     7927..7946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8143..8160
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8610..8629)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        complement(join(9161..9166,9167..9183,9184..9189))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    9459..9480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9495..9500,9501..9518,9519..9525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9533..9549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9538..9560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9557..9573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9557..9573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9592..9612
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9594..9612
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9641..9657
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9642..9658
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      9704..9713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9710..9712,9713..9715,9716..9985,1..444)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9710..9712,9713..9715,9716..9985,1..441)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9755..9776)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
       61 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      121 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      181 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      241 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      301 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      361 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      421 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gagcggccgc caccgcggtg
      481 gagctcgaat tcgaagcttt tgccattctc accggattca gtcgtcactc atggtgattt
      541 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
      601 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
      661 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
      721 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
      781 gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga
      841 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
      901 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
      961 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     1021 ccttcttcac gaggcagacc tcagcgccag aaggccgcca gagaggccga gcgcggccgt
     1081 gaggcttgga cgctagggca gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg
     1141 acgcggtgga aagggggagg ggatgttgtc tacatggctc tgctgtagtg agtgggttgc
     1201 gctccggcag cggtcctgat caatcgtcac cctttctcgg tccttcaacg ttcctgacaa
     1261 cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt
     1321 ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct
     1381 gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg
     1441 ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac
     1501 ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc
     1561 gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc
     1621 gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat
     1681 gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt
     1741 gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac
     1801 cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca
     1861 actttgtcat gcttgacact ttatcactga taaacataat atgtccacca acttatcagt
     1921 gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa
     1981 acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg
     2041 cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac
     2101 cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct gcgcacctgt
     2161 gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg
     2221 cgccactgtc gactacgcca tcatggcgac agcgcctttc ctttgggttc tctatatcgg
     2281 gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga
     2341 tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt
     2401 cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc
     2461 gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc
     2521 ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc
     2581 gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat
     2641 gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca
     2701 ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc
     2761 ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct
     2821 cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat
     2881 ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc
     2941 aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc
     3001 gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc
     3061 tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct
     3121 gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg
     3181 gaaacgatag ggacgtcata tggatccaac ccctccgctg ctatagtgca gtcggcttct
     3241 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     3301 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     3361 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     3421 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     3481 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     3541 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     3601 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     3661 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     3721 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     3781 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     3841 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     3901 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     3961 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     4021 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     4081 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     4141 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     4201 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     4261 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     4321 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     4381 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     4441 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     4501 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     4561 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     4621 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     4681 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     4741 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     4801 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     4861 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     4921 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     4981 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     5041 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     5101 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     5161 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     5221 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     5281 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     5341 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     5401 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     5461 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     5521 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5581 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     5641 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     5701 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     5761 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     5821 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     5881 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     5941 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     6001 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     6061 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     6121 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     6181 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     6241 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6301 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6361 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6421 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6481 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6541 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     6601 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     6661 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     6721 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     6781 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     6841 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     6901 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgct
     6961 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcaatggca acaacgttgc
     7021 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7081 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7141 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7201 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7261 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7441 cgttccactg agcgtcagac cccttaataa gatgatcttc ttgagatcgt tttggtctgc
     7501 gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc
     7561 tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac
     7621 ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa
     7681 ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga
     7741 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     7801 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     7861 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     7921 gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     7981 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc
     8041 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt
     8101 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     8161 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc
     8221 ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac
     8281 actccgctag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg
     8341 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta
     8401 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg
     8461 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt
     8521 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     8581 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     8641 aacgtcttgc tcgagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     8701 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     8761 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     8821 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     8881 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     8941 tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     9001 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
     9061 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     9121 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
     9181 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtccat gggcaaatat
     9241 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     9301 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtttttatg catgcgccca
     9361 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     9421 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     9481 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     9541 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     9601 cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaggtcga cggtatcgat
     9661 aagcttgata tcgaattcct gcagcccggg ggatccaccg gtcgccacca tggtgagcaa
     9721 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     9781 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     9841 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     9901 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     9961 cttcaagtcc gccatgcccg aaggc
//
