LOCUS       sequence-21201-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM
  TITLE     Interaction between P-TEFb and the C-terminal domain of RNA 
            polymerase II activates transcriptional elongation from sites 
            upstream or downstream of target genes.
  JOURNAL   Mol Cell Biol. 2002 Jan . 22(1):321-31.
  PUBMED    11739744
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Jan . 22(1):321-31."
COMMENT     Sequence Label: pG6(5'Pro) (P#1477)
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    289..383
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        complement(417..435)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(418..435)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(516..535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     635..657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(695..713)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaatnancat gggaatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gccaagctga tgaattcgag ctcggtacca agctttattg
      121 aggcttaagc agtgggttcc ctagctagcc agagagctcc caggctcaga tctggtctaa
      181 ccagagagac ccagtacagg caaaaagcag ctgcttatat gcagcatctg agggctcgcc
      241 actccccagt cccgcccagg ccacgcctcc ctggatctag agtctccgct cggaggacag
      301 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgctcgg aggacagtac
      361 tccgctcgga ggacagtact ccgacctgca ggcatgcaag cttcagctgc tcgagttcta
      421 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
      481 tctaacgaca atatgtccat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      541 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      601 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      661 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      721 gtaatgtcat gataataatg gttcttagac gtcaggtggc actttcggga aatgtgcgcg
      781 aaccctattg ttattttcta atacatcaat atgtatcgct cngaacatac ctgatatgct
      841 catatatgaa agaaatngat tcacttcnng cnctatcntt tggnntnct
//
