LOCUS       sequence-212133-        6427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LexA DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Chang HJ, Mayonove P, Zavala A, De Visch A, Minard P, Cohen-Gonsaud 
            M, Bonnet J
  TITLE     A Modular Receptor Platform To Expand the Sensing Repertoire of 
            Bacteria.
  JOURNAL   ACS Synth Biol. 2017 Oct 30. doi: 10.1021/acssynbio.7b00266.
  PUBMED    28946740
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Oct 30. doi: 10.1021/acssynbio.7b00266."
COMMENT     Sequence Label: pHJ2: LexA DBD
FEATURES             Location/Qualifiers
     source          1..6427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(292..1005,1006..1008))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1155..1182
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    1285..1301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1308..1324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1729..1800
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1816..1843
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2084..3166
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2103..2122)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(3318..4133)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4041..4060
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4125..4144
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(4491..5441)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5489..5711
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6203..6297)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cggataccct tactctgttg aaaacgaata gataggtttg ctcgtagttt accacggata
       61 cagacagtga taatcttagg cgcgccgaat cttgcttggc aaggttcttt ttttttttca
      121 aaaaagcacg aaaaaagcac ccataaggta aacttttgag tgcttttaaa tacgcttcac
      181 agtttcttct tcatttttaa caaaaaaccc agctcattga gctgggttta agctggcttg
      241 gcggcgtcct aagattacta ctgataacca ctgttgattg ggatacccgt attatttata
      301 aagttcgtcc ataccgtgag tgatacctgc tgctgtaaca aattcaagaa gtaccatatg
      361 gtcacgtttt tcgttaggat cttttgaaag aactgattga gtagaaaggt agtggttatc
      421 tggaagcaat actggtccgt ctccgattgg tgtattttgt tgataatgat cagccaattg
      481 aacagatcca tcttcaacat tgtgacgaat tttaaaatta gctttaatac cgtttttttg
      541 tttgtcagct gtgatataaa cattatgaga gttgaagttg tattcaagtt tgtgtcccaa
      601 aatgttacca tcttctttga aatcgattcc tttcaattcg atacgattaa caagagtgtc
      661 accttcgaat tttacttcag cacgtgtttt atatgttccg tcatctttaa atgaaattgt
      721 acgttcttgt acataacctt ctggcatagc agatttaaaa aaatcgtgac gtttcatatg
      781 atcaggataa cgtgaaaaac attgtactcc atatgtcaat gttgtaacaa gagtaggcca
      841 aggaacagga agttttccag ttgtacaaat gaatttaagt gtcaatttac cgttagttgc
      901 atcaccttca ccttcaccac gtacagaaaa tttgtgaccg ttaacatcac catccaattc
      961 aaccaagata ggtactacac ctgtaaaaag ttcttctcct tttgacattt caccaccttt
     1021 actagtccag cccctgtgag ttaccgtatg gatgtacggt acatccagtg acaacaaaga
     1081 tcaaccctat tttcggaaag agcctcgaag gaacggttat ttctgcgtag atctatctta
     1141 cacagcatca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag
     1201 aatataaaaa gccagattat taatccggct tttttattat ttaggcaact gaaacgattc
     1261 ggatcctgta ttactattct taaattgtga gcggataaca attgacattg tgagcggata
     1321 acaagatact gagcacagct agcgattaac taataaggag gacaaacatg aaagcgctga
     1381 ctgctcgtca gcaggaagta ttcgacctga tccgtgacca tatcagccaa accggcatgc
     1441 ctcctacgcg tgcagaaatt gcacagcgcc tgggctttcg ctccgcctct tccgctgaag
     1501 agcatctgaa agcgctggct cgcaaaggtg tgatcgaaat cgtatccggt gctagccgtg
     1561 gtattcgtct gctgcaggaa gaagaggaag gtctgccgct ggttggccgt gtagctgctg
     1621 gcgaaccgta ataaacttta tctgagaata gtcaatcttc ggaaatccca ggtggcatgc
     1681 taaaagtctc gtaaagcgtt ctatcaataa cccgttggtg ccaggcatca aataaaacga
     1741 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1801 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac cgtctcagaa
     1861 tcggccgtga acaataaaat agtttcggtt tgcatttaaa tcttacatat gtaatacttt
     1921 caaagactac atttgtaaga tttgatgttt gagtcggctg aaagatcgta cgtaccaatt
     1981 attgtttcgt gattgttcaa gccataacac tgtagggata gtggaaagag tgcttcatct
     2041 ggttacgatc aatcaaatat tcaaacggag ggagacgatt ttgatgaaac cagtaacgtt
     2101 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     2161 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     2221 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     2281 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     2341 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     2401 ctgtaaaacg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     2461 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     2521 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     2581 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     2641 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     2701 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     2761 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     2821 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     2881 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     2941 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     3001 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     3061 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3121 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgataat aaaaggtccc
     3181 gtctgaactt actgtgaatt cgactaatta ttgaccactt ccgagtagaa tcgtgcttca
     3241 gtaagagctc actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat
     3301 gatttctgct agtattatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     3361 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     3421 tcaccgaggc agttccaaag aatggcaagg tcctggtaac ggtctgcgat tccgacccgt
     3481 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     3541 tcaccatgag tgacgactga atccggtgag aatggcaaga gcttgtgcat ttctttccag
     3601 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     3661 ttattcatgc gtgattgcgc ctgagcaaga cgaaatacac gatcgctgtt aaaaggacaa
     3721 ttacaaacag gaatcgaatg taaccggcgc aggaacacgg ccagcgcatc aacaatattt
     3781 tcacctgaat caggatattc ttctaatacc tggaaggctg ttttcccagg aatcgcggtg
     3841 gtgagtaacc acgcatcatc aggagtacgg ataaaatgct tgatggtcgg gagaggcata
     3901 aactccgtca gccagttgag acggaccatc tcatctgtaa catcattggc aacgctacct
     3961 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc
     4021 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcgtccatg
     4081 ttggagttta agcgcggacg ggagcaagac gtttcccgtt gaatatggct cataacaccc
     4141 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     4201 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     4261 tttgctgagt tgaaggatca gctctagtag ttacattgtc gatctgttca tggtgaacag
     4321 ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     4381 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     4441 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     4501 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     4561 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     4621 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     4681 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     4741 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     4801 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     4861 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     4921 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     4981 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     5041 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     5101 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     5161 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     5221 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     5281 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     5341 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     5401 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     5461 tgattttaat cactatacca attgagatgg gctagtcaat gataattaca tgtccttttc
     5521 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     5581 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     5641 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     5701 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     5761 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     5821 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     5881 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     5941 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     6001 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     6061 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     6121 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
     6181 tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     6241 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     6301 gcgagctcgt aaacttggtc tgacagctct agctccggca aaaaaacggg caaggtgtca
     6361 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgtttgcca cctgacgtct
     6421 aagaact
//
