LOCUS       sequence-212157-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral  expression of shRNA targeting PBRM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Gao W, Li W, Xiao T, Liu XS, Kaelin WG Jr
  TITLE     Inactivation of the PBRM1 tumor suppressor gene amplifies the 
            HIF-response in VHL-/- clear cell renal carcinoma.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1027-1032. doi: 
            10.1073/pnas.1619726114. Epub 2017 Jan 12.
  PUBMED    28082722
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1619726114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-01-31- 31"; volume: "114"; issue: "5"; pages: 
            "1027-1032"
COMMENT     Sequence Label: shPBRM1 #122-Tet-pLKO-puro
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        315..409
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     326..345
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    391..409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    509..626
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        675..1185
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1104..1122
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          1194..1766
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1774..1793
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1821..1839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1850..2470
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1923..1942)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2505..3078
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2672..2689)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2899..2918
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3098..3697
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3098..3117)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3594..3614
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3825..4058
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4130..4251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4167..4186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4221..4240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4291..4426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4447..4465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4475..4492)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4475..4491)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4484..4506)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4633..5088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4720..4739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4930..4951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5114..5218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5219..5287,5288..6079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5437..5456)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6250..6838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6739..6758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6992..7009
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7126..7147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7162..7167,7168..7185,7186..7192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7200..7216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7205..7227
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7224..7240
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7224..7240
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7259..7279
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7261..7279
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7307..7533
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7534..7714
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7761..7886
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8379..8612
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(8797..8815,1..26)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
       61 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
      121 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
      181 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      241 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
      301 ctcgagcggc cgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga
      361 aatccctatc agtgatagag acttataagt tccctatcag tgatagagac accgggttag
      421 aaggtggaga tgataactcg agttatcatc tccaccttct aactttttaa ttctcgacct
      481 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
      541 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      601 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
      661 ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
      721 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
      781 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
      841 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
      901 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
      961 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     1021 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     1081 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     1141 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tctgtgagtt
     1201 tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg
     1261 gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct
     1321 catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt
     1381 tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta
     1441 aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag
     1501 gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta
     1561 aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg
     1621 gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata
     1681 cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg
     1741 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     1801 atcattttgg caaagaattg taatacgact cactataggg cgaattgata tgtctagatt
     1861 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
     1921 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
     1981 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
     2041 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
     2101 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
     2161 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
     2221 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
     2281 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
     2341 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
     2401 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
     2461 aagtgggtcc gcgtacagcg gctcccggga gttctaggga tctgcccctc tccctccccc
     2521 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2581 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2641 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2701 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2761 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2821 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2881 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2941 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     3001 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     3061 tgaaaaacac gatgataagg atccaccgga gcttaccatg accgagtaca agcccacggt
     3121 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     3181 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     3241 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3301 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3361 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3421 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3481 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3541 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3601 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3661 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     3721 cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa tgacttacaa
     3781 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3841 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3901 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3961 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4021 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4081 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4141 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4261 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4321 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4381 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     4441 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     4501 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4561 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4621 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4681 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4741 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4801 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4861 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4921 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4981 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5041 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     5101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5221 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5281 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5341 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5401 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5461 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5521 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5581 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5641 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5701 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5761 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5821 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5881 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5941 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6001 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6061 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6361 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6901 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6961 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7021 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7081 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7141 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7201 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     7261 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     7321 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     7381 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     7441 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     7501 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     7561 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7681 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     7741 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     7801 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     7861 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     7921 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     7981 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8041 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8101 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8161 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8221 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8281 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     8341 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     8401 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     8461 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     8521 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     8581 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     8641 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     8701 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     8761 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attat
//
