LOCUS       sequence-212191-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Two SpyTags linked by an elastin-like polypeptide for hydrogel 
            formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Wieduwild R, Howarth M
  TITLE     Assembling and decorating hyaluronan hydrogels with twin protein 
            superglues to mimic cell-cell interactions.
  JOURNAL   Biomaterials. 2018 Oct;180:253-264. doi: 
            10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17.
  PUBMED    30053659
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomaterials."; date: "2018-10"; volume: "180:253-264. doi"; pages:
            " 10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17"
COMMENT     Sequence Label: pQE80L SpyTag-ELP-SpyTag
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..78
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             634..672
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaaaggca gcagccatca tcatcatcat cacgtcgacg cccatattgt catggttgat
       61 gcatacaagc cgacgaagct cgacggccac ggcgtgggtg ttccgggcgt cggtgtgccg
      121 ggtgtgggtg tgccgggcga aggtgtgccg ggcgtcggtg tgccgggtgt tggtgttccg
      181 ggcgttggtg tgccgggcgt tggcgtgccg ggcgagggtg tgccgggcgt tggtgttccg
      241 ggcgtgggtg tgccgggcgt gggcgtgccg ggcgtcggtg ttccgggcga gggtgtgccg
      301 ggcgtaggtg tgccgggcgt tggtgagctc tatgcggtta ccggccgtgg tcgtgccccg
      361 gcctgctctg ccccgatcgc cactagtgtg ccgggcgtcg gcgtgccggg cgtaggtgtt
      421 ccgggcgagg gtgttccggg cgttggtgtg ccgggcgtcg gcgtgccggg cgtgggtgtt
      481 ccgggcgtag gtgtgccggg cgagggtgtg ccgggcgtgg gcgtgccggg cgtaggtgtt
      541 ccgggcgtag gtgttccggg cgtaggtgtt ccgggtgaag gcgtgccggg cgttggtgtg
      601 ccgggtgtgg gcgtgccggg cgggctgctc gacgcccata ttgtcatggt tgatgcatac
      661 aagccgacga agctcgagtg gaagaaataa
//
