LOCUS       sequence-212237-        5493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An AAV vector that expresses cyan pre-eGRASP(p30) under the CaMKIIa 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Choi JH, Sim SE, Kim JI, Choi DI, Oh J, Ye S, Lee J, Kim T, Ko HG, 
            Lim CS, Kaang BK
  TITLE     Interregional synaptic maps among engram cells underlie memory 
            formation.
  JOURNAL   Science. 2018 Apr 27;360(6387):430-435. doi: 
            10.1126/science.aas9204.
  PUBMED    29700265
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-04-27- 27"; volume: "360"; 
            issue: "6387"; pages: "430-435"
COMMENT     Sequence Label: pAAV-CWB-cyan pre-eGRASP(p30)
FEATURES             Location/Qualifiers
     source          1..5493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      522..531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(645..666)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    1895..2483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1948..1968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2366..2377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2496..2720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2728..2868
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2728..2857
                     /label=AAV2 ITR
     rep_origin      2943..3398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3030..3049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3240..3261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3415..3434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3534..3556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3594..3612)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3680..3784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3785..3853,3854..4645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4003..4022)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4816..5404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5305..5324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5466..5493,1..102)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag
       61 cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggccgc agatgctgca
      121 cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc ccctcagtac atcaccctgg
      181 gggaacaggg tccacttgct cctgggccca cacagtcctg cagtattgtg tatataaggc
      241 cagggcaaag aggagcaggt tttaaagtga aaggcaggca ggtgttgggg aggcagttac
      301 cggggcaacg ggaacagggc gtttcggagg tggttgccat ggggacctgg atgctgacga
      361 aggctcgcga ggctgtgagc agccacagtg ccctgctcag aagccccaag ctcgtcagtc
      421 aagccggttc tccgtttgca ctcaggagca cgggcaggcg agtggcccct agttctgggg
      481 gcagcgtgaa aagacggtat cgataagctt gattcgagct agccaccatg gagaccgaca
      541 ccctcctgct atgggtgctg ctgctctggg tgcccggcag caccggcgac gcaccggtcg
      601 gaggcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      661 gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg cgagggcgat gccaccatcg
      721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      781 tcgtgaccac cctgagctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
      841 ggcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctcct
      901 tcaaggacga cggcaagtac aagacccgcg ccgtggtgaa gttcgagggc gacaccctgg
      961 tgaaccgcat cgagctgaag ggcaccgact tcaaggagga cggcaacatc ctggggcaca
     1021 agctggagta caacttcaac agcggcaacg tctatatcac cgccgacaag cagaagaacg
     1081 gcatcaaggc caacttcacc gtgcgccaca acgtggagga cggcagcgtg cagctcgccg
     1141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1201 acctgagcac ccagagcgtg ctgagcaaag accccaacga gaagaccggt ggatctggag
     1261 gcagtggcgg atctagagct cctacaaagc ctccaccact gccacctgga ggaggttccg
     1321 gaggcggaag cggtaccgaa gtgccttcct ctatgacaac tgagtcgaca gccactgcca
     1381 tgcagtccga gatgtccacc tcaatcatgg agaccaccac aaccctggct accagcacag
     1441 ctcgacgagg aaagcccccc acaaaggagc ctatcagcca gaccacggat gatatccttg
     1501 tggcctcggc agagtgtccc agcgacgatg aggacattga cccctgtgag ccgagctcag
     1561 gtgggttagc caaccccacc cgagtgggcg gccgcgaacc atacccaggc tcggcagagg
     1621 tgatccggga gtctagcagt accactggca tggtggtggg gattgtcgca gcagcagctc
     1681 tgtgcatcct catcctcctc tatgccatgt acaagtacag aaaccgggat gaaggctcat
     1741 atcacgtgga tgagagtcga aactacatca gtaactcagc acagtccaat ggggctgtgg
     1801 tcaaggagaa gcagcccagc agtgcgaaaa gcgccaacaa aaacaagaag aacaaggata
     1861 aggagtatta tgtctgaatt cctgcagccc cgataatcaa cctctggatt acaaaatttg
     1921 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1981 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2041 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2101 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2161 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2221 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2281 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2341 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2401 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2461 ctccctttgg gccgcctccc cgcatcgggg ggatcctgtg ccttctagtt gccagccatc
     2521 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     2581 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     2641 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     2701 ggatgcggtg ggctctatgg cggccgcagg aacccctagt gatggagttg gccactccct
     2761 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2821 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     2881 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     2941 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3001 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3061 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3121 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     3181 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3241 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     3301 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     3361 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     3421 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3481 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3541 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     3601 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4201 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5461 catgtcctgc aggcagctgc gcgctcgctc gct
//
