LOCUS       sequence-21263-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN R-Ras 41A
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           95..134
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             170..196
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc gggggatctt ggtggcgtga aactcccgca
      121 cctcttcggc cagcgccttg tagaagcgcg tatggcttct agctatcctt atgacgtgcc
      181 tgactatgcc agcctgggag gaccttctag cggatccgtc gacatgagct ctggtgctgc
      241 ttccggtaca ggtcgtggtc gtccacgtgg tggaggtcca ggtcccgggg accccccgcc
      301 cagcgagaca cacaagctgg tggtcgtggg cggcggcggc gtggccaaga gcgcgctgac
      361 catccagttc atccagtcct acttcgtgtc tgactacgac cccactattg aggactccta
      421 cacgaagatc tgcagtgtgg atggcatccc agcccggctg gacatcctgg acaccgcggg
      481 ccaggaagag ttcggggcca tgagagagca gtacatgcgt gctggccacg gcttcctgct
      541 ggtgttcgcc attaatgacc ggcagagttt caacgaggtg ggcaagctct tcacgcagat
      601 tctgcgggtc aaggaccgcg acgacttccc cgttgtgttg gtcgggaaca angcagatct
      661 ggagtcacag cgccaggtcc cccgatcaga agcctctgcc ttcggcgcct cccaccacgt
      721 ggcctacttt gaggcctcgg ccaaactgcg tctcaacgt
//
