LOCUS       sequence-212640-       16244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible expression in mammalian cells of Ds1 protein fused to 
            mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16244)
  AUTHORS   Loza O, Heemskerk I, Gordon-Bar N, Amir-Zilberstein L, Jung Y, 
            Sprinzak D
  TITLE     A synthetic planar cell polarity system reveals localized feedback 
            on Fat4-Ds1 complexes.
  JOURNAL   Elife. 2017 Aug 18;6. pii: 24820. doi: 10.7554/eLife.24820.
  PUBMED    28826487
REFERENCE   2  (bases 1 to 16244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-18"; volume: "6. pii: 24820. doi"; pages: " 
            10.7554/eLife.24820"
COMMENT     Sequence Label: pcDNA5-T/O-Ds1-mCherry
FEATURES             Location/Qualifiers
     source          1..16244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9924..10637
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(10069..10087)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10337..10356
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(10675..10692)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10681..10905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10951..11379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11038..11057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11248..11269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11388..11408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11393..11722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11573..11708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11635..11654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11771..12796
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             11771..12796
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    12926..13047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12963..12982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13017..13036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13096..13112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13096..13112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13109..13131)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13120..13136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13144..13150,13151..13168,13169..13174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13189..13210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13327..13344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13498..14086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13578..13597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14257..15048,15049..15117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14880..14899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15118..15222)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15297..15316)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        15488..15867
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15868..16071
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16022..16042
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    16073..16091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16094..16112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     16122..16146
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggccgctcgc caccatgcag aaggagctgg gcattgtgcc ttcctgccct ggcatgaaga
       61 gccccaggcc ccacctcctg ctaccattgc tgctgctgct gctgctgctg ctgggggctg
      121 gggtgccagg tgcctggggt caggctggga gcctggactt gcagattgat gaggagcagc
      181 cagcgggtac actgattggc gacatcagtg cggggcttcc ggcaggcacg gcagctcctc
      241 tcatgtactt catctctgcc caagagggca gcggcgtggg cacagacctg gccattgacg
      301 aacacagtgg ggtcgtccgt acagcccgtg tcttggaccg tgagcagcgg gaccgctacc
      361 gcttcactgc agtcactcct gatggtgcca ccgtagaagt tacagtgcga gtggctgaca
      421 tcaacgacca tgctccagcc ttcccacagg ctcgggctgc cctgcaggta cctgagcata
      481 cagcttttgg cacccgctac ccactggagc ctgctcgtga tgcagatgct gggcgtctgg
      541 gaacccaggg ctatgcgcta tctggtgatg gggctggaga gaccttccgg ctggagacac
      601 gccccggtcc agatgggact ccagtacctg agctggtagt tactggggaa ctggaccgag
      661 agaaccgctc acactatatg ctacagctgg aggcctatga tggtggttca cccccccgga
      721 gggcccaggc cctgctggac gtgacactgc tggacatcaa tgaccatgcc ccggctttca
      781 atcagagccg ctaccatgct gtggtgtctg agagcctggc ccctggcagt cctgtcttgc
      841 aggtgttcgc atctgatgcc gatgctggtg tcaatggggc tgtgacttac gagatcaacc
      901 ggaggcagag cgagggtgat ggacccttct ccatcgacgc acacacgggg ctgctgcagt
      961 tagagcggcc actggacttt gagcagcggc gggtccatga actggtggtg caagcacgag
     1021 atggtggggc tcaccctgag ctgggctcgg cctttgtgac tgtgcatgtg cgagatgcca
     1081 atgacaatca gccctccatg actgtcatct ttctcagtgc agatggctcc ccccaagtgt
     1141 ctgaggccgc cccacctgga cagctcgttg ctcgcatctc tgtgtcagac ccagatgatg
     1201 gtgactttgc ccatgtcaat gtgtccctgg aaggtggaga gggccacttt gccctaagca
     1261 cccaagacag cgtcatctat ctggtgtgtg tggctcggcg gctggatcga gaggagaggg
     1321 atgcctataa cttgagggtt acagccacag actcaggctc acctccactg cgggctgagg
     1381 ctgcctttgt gctgcacgtc actgatgtca acgacaatgc acctgccttt gaccgccagc
     1441 tctaccgacc tgagcccctg cctgaggttg cgctgcctgg cagctttgta gtgcgggtga
     1501 ctgctcggga tcctgaccaa ggcaccaatg gtcaggtcac ttatagccta gcccctggcg
     1561 cccacaccca ctggttctcc attgacccca cctcaggcat tatcactacg gctgcctcac
     1621 tggactatga gttggaacct cagccacagc tgattgtggt ggccacagat ggtggcctgc
     1681 cccctctagc ctcctctgcc acagttagcg tggccctgca agatgtgaat gataatgagc
     1741 cccaattcca gaggactttc tacaatgcct cactgcctga gggcacccag cctggaactt
     1801 gcttcctgca ggtgacagcc acagacgcgg atagtggccc atttggcctc ctctcctatt
     1861 ccttgggtgc tggacttggg tcctccggat ctcccccatt ccgcattgat gcccatagcg
     1921 gtgatgtgtg cacaacccgg accctggacc gtgaccaggg gccctcaagc tttgacttca
     1981 cagtgacagc tgtggatggg ggaggcctca agtccatggt atatgtgaag gtgtttctgt
     2041 cagacgagaa tgacaaccct cctcagtttt atccacggga gtatgctgcc agtataagtg
     2101 cccagagtcc accaggcaca gctgtgctga ggttgcgtgc ccatgaccct gaccagggat
     2161 cccatgggcg actctcctac catatcctgg ctggcaacag ccccccactt tttaccttgg
     2221 atgagcaatc agggctgttg acagtagcct ggcccttggc cagacgggcc aattctgtgg
     2281 tgcagctgga gatcggggct gaggacggag gtggcctaca ggcagaaccc agtgcccgag
     2341 tggacatcag cattgtgcct ggaaccccca caccacccat atttgagcaa ctacagtatg
     2401 ttttttctgt gccagaggat gtggcaccag gcaccagtgt gggcatagtc caggcacaca
     2461 acccaccagg tcgcttggca cctgtgaccc tttccctatc aggtggggat ccccgaggac
     2521 tcttctccct agatgcggta tcaggactgt tgcaaacact tcgccctctg gaccgggagc
     2581 tactgggacc agtgttggag ctggaggtgc gagcaggcag tggagtgccc ccagctttcg
     2641 ctgtagctcg ggtgcgtgtg ctgctggatg atgtgaatga caactcccct gcctttcctg
     2701 cacctgaaga cacggtattg ctaccaccaa acactgcccc agggactccc atctatacac
     2761 tgcgggctct tgaccccgac tcaggtgtta acagtcgagt cacctttacc ctgcttgctg
     2821 ggggtggtgg agccttcacc gtggacccca ccacaggcca tgtacggctt atgaggcctc
     2881 tggggccctc aggagggcca gcccatgagc tggagctgga ggcccgggat gggggctccc
     2941 caccacgcac cagccacttt cgactacggg tggtggtaca ggatgtggga acccgtgggc
     3001 tggctccccg attcaacagc cctacctacc gtgtggacct gccctcaggc accactgctg
     3061 gaactcaggt cctgcaagtg caggcccaag caccagatgg gggccctatc acctatcacc
     3121 ttgcagcaga gggagcaagt agcccctttg gcctggagcc acagagtggg tggctatggg
     3181 tgcgggcagc actagaccgt gaggcccagg aattgtacat actgaaggta atggcagtgt
     3241 ctgggtccaa agctgagttg gggcagcaga caggcacagc caccgtgagg gtcagcatcc
     3301 tcaaccagaa tgaacacagt ccccgcttgt ctgaggatcc caccttcctg gctgtggctg
     3361 agaaccagcc cccagggacc agcgtgggcc gagtctttgc cactgaccga gactcaggac
     3421 ccaatggacg tctgacctac agcctgcaac agctgtctga agacagcaag gccttccgca
     3481 tccaccccca gactggagaa gtgaccacac tccaaaccct ggaccgtgag cagcagagca
     3541 gctatcagct cctggtgcag gtgcaggatg gagggagccc accccgcagc accacaggca
     3601 ctgtgcatgt tgcagtgctt gacctcaacg acaacagccc cacgttcctg caggcttcag
     3661 gagctgctgg tgggggcctc cctatacagg taccagaccg cgtgcctcca ggaacactgg
     3721 tgacgactct gcaggcgaag gatccagatg agggggagaa tgggaccatc ttgtacacgc
     3781 taactggtcc tggctcagag cttttctctc tgcaccctca ctcaggggag ctgctcactg
     3841 cagctcccct gatccgagca gagcggcccc actatgtgct gacactgagt gctcatgacc
     3901 aaggcagccc tcctcgaagt gccagcctcc agctgctggt gcaggtgctt ccctcagctc
     3961 gcttggccga gccgccccca gatctcgcag agcgggaccc agcggcacca gtgcctgtcg
     4021 tgctgacggt gacagcagct gagggactgc ggcccggctc tctgttgggc tcggtggcag
     4081 cgccagagcc cgcgggtgtg ggtgcactca cctacacact ggtgggcggt gccgatcccg
     4141 agggcacctt cgcgctggat gcggcctcag ggcgcttgta cctggcgcgg cccctggact
     4201 tcgaagctgg cccgccgtgg cgcgcgctca cggtacgcgc tgaggggccg ggaggcgcgg
     4261 gcgcgcggct gctgcgagtg caggtgcaag tgcaggacga gaatgagcat gcgcccgcct
     4321 ttgcgcgcga cccgctggcg ctggcgctgc cagagaaccc ggagcccggc gcagcgctgt
     4381 acactttccg cgcgtcggac gccgacggcc ccggccccaa tagcgacgtg cgctaccgcc
     4441 tgctgcgcca ggagccgccc gtgccggcgc ttcgcctgga cgcgcgcacc ggggcgctca
     4501 gcgctccgcg cggcctggac cgagagacca ctcccgcgct gctgctgctg gtggaagcca
     4561 ccgaccggcc cgccaacgcc agccgccgtc gtgcagcgcg cgtttcagcg cgcgtcttcg
     4621 tcacggatga gaatgacaac gcgcctgtct tcgcctcgcc gtcacgcgtg cgcctcccag
     4681 aggaccagcc gcctgggccc gcggccctgc acgtggtagc ccgggacccg gatctgggcg
     4741 aggctgcacg cgtgtcctat cggctggcat ctggcgggga cggccacttc cggctgcact
     4801 caagcactgg agcgctgtcc gtggtgcggc cgttggaccg cgaacaacga gctgagcacg
     4861 tactgacagt ggtggcctca gaccacggct ccccgccgcg ctcggccacg caggtcctga
     4921 ccgtcagtgt cgctgacgtc aacgacgagg cgcctacttt ccagcagcag gagtacagcg
     4981 tcctcttgcg tgagaacaac cctcctggca catctctgct caccctgcga gcaaccgacc
     5041 ccgacgtggg ggccaacggg caagtgactt atggaggcgt ctctagcgaa agcttttctc
     5101 tggatcctga cactggtgtt ctcacgactc ttcgggccct ggatcgagag gaacaggagg
     5161 agatcaacct gacagtgtat gcccaggaca ggggctcacc tcctcagtta acgcatgtca
     5221 ctgttcgagt ggctgtggag gatgagaatg accatgcacc aacctttggg agtgcccatc
     5281 tctctctgga ggtgcctgag ggccaggacc cccagaccct taccatgctt cgggcctctg
     5341 atccagatgt gggagccaat gggcagttgc agtaccgcat cctagatggg gacccatcag
     5401 gagcctttgt cctagacctt gcttctggag agtttggcac catgcggcca ctagacagag
     5461 aagtggagcc agctttccag ctgaggatag aggcccggga tggaggccag ccagctctca
     5521 gtgccacgct gcttttgaca gtgacagtgc tggatgccaa tgaccatgct ccagcctttc
     5581 ctgtgcctgc ctactcggtg gaggtgccgg aggatgtgcc tgcagggacc ctgctgctgc
     5641 agctacaggc tcatgaccct gatgctggag ctaatggcca tgtgacctac tacctgggcg
     5701 ccggtacagc aggagccttc ctgctggagc ccagctctgg agaactgcgc acagctgcag
     5761 ccttggacag agaacagtgt cccagctaca ccttttctgt gagtgcagtg gatggtgcag
     5821 ctgctgggcc cctaagcacc acagtgtctg tcaccatcac ggtgcgcgat gtcaatgacc
     5881 atgcacccac cttccccacc agtcctctgc gcctacgtct gccccgccca ggccccagct
     5941 tcagtacccc aaccctggct ctggccacac tgagagctga agatcgtgat gctggtgcca
     6001 atgcttccat tctgtaccgg ctggcaggca caccacctcc tggcactact gtggactctt
     6061 acactggtga aatccgcgtg gcccgctctc ctgtagctct aggcccccga gatcgtgtcc
     6121 tcttcattgt ggccactgat cttggccgtc cagctcgctc tgccactggt gtgatcattg
     6181 ttggactgca gggggaagct gagcgtggac cccgctttcc ccgggctagc agtgaggcta
     6241 cgattcgtga gaatgcgccc ccagggactc ctattgtctc ccccagggcc gtccatgcag
     6301 gaggcacaaa tggacccatc acctacagca ttctcagtgg gaatgagaaa gggacattct
     6361 ccatccagcc tagtacaggt gccatcacag ttcgctcagc agaggggcta gacttcgagg
     6421 tgagtccacg gctgcgactg gtgctgcagg cagagagtgg aggagccttt gccttcactg
     6481 tgctgaccct gaccctgcaa gatgccaacg acaatgctcc ccgtttcctg cggccccatt
     6541 atgtggcctt ccttcctgag tcccggccct tggaggggcc cctgctgcag gtggaggcgg
     6601 atgacctgga tcaaggctct ggaggacaga tttcctacag tctggctgca tcccagccgg
     6661 cacgtggatt gttccacgta gacccaacca caggcactat cactaccaca gccatcctgg
     6721 accgtgagat ctgggctgaa acacggttgg tgctgatggc cacagacaga gggagcccag
     6781 ccctggtggg ctcagctacc ttgacggtga tggtcatcga caccaatgac aatcgcccca
     6841 ccatccccca accctgggag ctccgagtgt cagaagatgc gttattgggc tcagagattg
     6901 cacaggtaac agggaatgat gtggactcag gacccgtgct gtggtatgtg ctaagcccat
     6961 ctgggcccca ggatcccttc agtgttggcc gctatggagg ccgtgtctcc ctcacggggc
     7021 ccctggactt tgagcagtgt gaccgctacc agctgcagct gctggcacat gatgggcctc
     7081 atgagggccg tgccaacctc acagtgcttg tggaggatgt caatgacaat gcacctgcct
     7141 tctcacagag cctctaccag gtaatgctgc ttgagcacac acccccaggc agtgccattc
     7201 tctccgtctc tgccactgat cgggactcag gtgccaacgg tcacatttcc taccacctgg
     7261 cttcccctgc cgatggcttc agtgttgacc ccaacaatgg gaccctgttc acaatagtgg
     7321 gaacagtggc cttgggccat gacgggtcag gagcagtgga tgtggtgctg gaagcacgag
     7381 accacggggc tccaggccgg gcagcacgag ccacagtgca cgtgcagctg caggaccaga
     7441 acgaccacgc cccgagcttc acattgtcac actaccgtgt ggctgtgact gaagacctgc
     7501 cccctggctc cactctgctc accctggagg ctacagatgc tgatggaagc cgcagccatg
     7561 ccgctgtgga ctacagcatc atcagtggca actggggccg agtcttccag ctggaaccca
     7621 ggctggctga ggctggggag agtgctggac caggcccccg ggcactgggc tgcctggtgt
     7681 tgcttgaacc tctagacttt gaaagcctga cacagtacaa tctaacagtg gctgcagctg
     7741 accgtgggca gccaccccaa agctcagtcg tgccagtcac tgtcactgta ctagatgtca
     7801 atgacaaccc acctgtcttt acccgagcat cctaccgtgt gacagtacct gaggacacac
     7861 ctgttggagc tgagctgctg catgtagagg cctctgacgc tgaccctggc cctcatggcc
     7921 tcgtgcgttt cactgtcagc tcaggcgacc catcagggct ctttgagctg gatgagagct
     7981 caggcacctt gcgactggcc catgccctgg actgtgagac ccaggctcga catcagcttg
     8041 tagtacaggc tgctgaccct gctggtgcac actttgcttt ggcaccagtg acaattgagg
     8101 tccaggatgt gaatgatcat ggcccagcct tcccactgaa cttactcagc accagcgtgg
     8161 ccgagaatca gcctccaggc actctcgtga ccactctgca tgcaatcgac ggggatgctg
     8221 gggcttttgg gaggctccgt tacagcctgt tggaggctgg gccaggacct gagggccgtg
     8281 aggcatttgc actgaacagc tcaacagggg agttgcgtgc gcgagtgccc tttgactatg
     8341 agcacacaga aagcttccgg ctgctggtgg gtgctgctga tgctgggaat ctctcagcct
     8401 ctgtcactgt gtcggtgcta gtgactggag aggatgagta tgaccctgta tttctggcac
     8461 cagctttcca cttccaagtg cccgaaggtg cccggcgtgg ccacagcttg ggtcacgtgc
     8521 aggccacaga tgaggatggg ggtgccgatg gcctggttct gtattccctt gccacctctt
     8581 ccccctattt tggtattaac cagactacag gagccctgta cctgcgggtg gacagtcggg
     8641 caccaggcag cggaacagcc acctctgggg gtgggggccg gacccggcgg gaagcaccac
     8701 gggagctgag gctggaggtg atagcacggg ggcctctgcc tggttcccgg agtgccacag
     8761 tgcctgtgac cgtggatatc acccacaccg cactgggcct ggcacctgac ctcaacctgc
     8821 tattagtagg ggccgtggca gcctccttgg gagttgtggt ggtgcttgca ctggcagccc
     8881 tggtcctagg acttgttcgg gcccgtagcc gcaaggctga ggcagcccct ggcccaatgt
     8941 cacaggcagc acccctagcc agtgactcac tgcagaaact gggccgggag ccacctagtc
     9001 caccaccctc tgagcacctc tatcaccaga ctcttcccag ctatggtggg ccaggagctg
     9061 gaggacccta cccccgtggt ggctccttgg acccttcaca ttcaagtggc cgaggatcag
     9121 cagaggctgc agaggatgat gagatccgca tgatcaatga gttcccccgt gtggccagtg
     9181 tggcctcctc tctggctgcc cgtggccctg actcaggcat ccagcaggat gcagatggtc
     9241 tgagtgacac atcctgcgaa ccacctgccc ctgacacctg gtataagggc cgaaaggcag
     9301 ggctgctgct gccaggtgca ggagccactc tctacagaga ggaggggccc ccagccactg
     9361 ccacagcctt cctggggggc tgtggcctga gccctgcacc cactggggac tatggcttcc
     9421 cagcagatgg caagccatgt gtggcaggtg cgctgacagc cattgtggcc ggcgaggagg
     9481 agctccgtgg cagctataac tgggactacc tgctgagctg gtgccctcag ttccaaccac
     9541 tggccagtgt cttcacagag atcgctcggc tcaaggatga agctcggcca tgtcccccag
     9601 ctccccgtat cgacccacca cccctcatca ctgccgtggc ccacccagga gccaagtctg
     9661 tgccccccaa gccagcaaac acagctgcag cccgggccat cttcccacca gcttctcacc
     9721 gctcccccat cagccatgaa ggctccctgt cctcagctgc catgtccccc agcttctcac
     9781 cctctctgtc tcctctggct gctcgctcac ccgttgtctc accatttggg gtggcccagg
     9841 gtccctcagc ctcagcactc agcgcagagt ctggcctgga gccacctgat gacacggagc
     9901 tgcacatcac cggtggctct ggcatggtga gcaagggcga ggaggataac atggccatca
     9961 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
    10021 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
    10081 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
    10141 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
    10201 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
    10261 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
    10321 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
    10381 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
    10441 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
    10501 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
    10561 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
    10621 agctgtacaa gtcttgactc gagtctagag ggcccgttta aacccgctga tcagcctcga
    10681 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10741 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10801 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10861 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    10921 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    10981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    11041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    11161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    11221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    11281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    11341 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
    11401 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    11461 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    11521 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    11581 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    11641 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    11701 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    11761 tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
    11821 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
    11881 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
    11941 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
    12001 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
    12061 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
    12121 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
    12181 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
    12241 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
    12301 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
    12361 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
    12421 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
    12481 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
    12541 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
    12601 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
    12661 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
    12721 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
    12781 gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
    12841 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
    12901 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
    12961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13021 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    13081 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    13141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    13201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    13261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    13321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    13381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    13441 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    13501 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    13561 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    13621 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    13681 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    13741 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    13801 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    13861 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    13921 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    13981 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggttggtt
    14041 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    14101 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    14161 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    14221 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    14281 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    14341 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    14401 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    14461 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    14521 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    14581 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    14641 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    14701 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    14761 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    14821 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    14881 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    14941 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    15001 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    15061 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    15121 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    15181 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    15241 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    15301 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    15361 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    15421 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
    15481 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
    15541 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
    15601 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
    15661 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
    15721 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    15781 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    15841 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
    15901 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
    15961 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
    16021 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc
    16081 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
    16141 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
    16201 tccagcctcc ggactctagc gtttaaactt aagcttggta ccgc
//
