LOCUS       sequence-212717-        5268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive promoter expressing araE on RK2 origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF
  TITLE     Genetic tools for reliable gene expression and recombineering in 
            Pseudomonas putida.
  JOURNAL   J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 
            10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
  PUBMED    29299733
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. 
            doi: 10.1007/s10295-017-2001-5."
COMMENT     Sequence Label: pRK2-AraE
FEATURES             Location/Qualifiers
     source          1..5268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1617..2765)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      3158..3689
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(3801..3895)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4166..4699)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEVVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4888..4893,4894..4910,4911..4916))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5169..5203
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
ORIGIN
        1 atggttacta tcaatacgga atctgcttta acgccacgtt ctttgcggga tacgcggcgt
       61 atgaatatgt ttgtttcggt agctgctgcg gtcgcaggat tgttatttgg tcttgatatc
      121 ggcgtaatcg ccggagcgtt gccgttcatt accgatcact ttgtgctgac cagtcgtttg
      181 caggaatggg tggttagtag catgatgctc ggtgcagcaa ttggtgcgct gtttaatggt
      241 tggctgtcgt tccgcctggg gcgtaaatac agcctgatgg cgggggccat cctgtttgta
      301 ctcggttcta tagggtccgc ttttgcgacc agcgtagaga tgttaatcgc cgctcgtgtg
      361 gtgctgggca ttgctgtcgg gatcgcgtct tacaccgctc ctctgtatct ttctgaaatg
      421 gcaagtgaaa acgttcgcgg taagatgatc agtatgtacc agttgatggt cacactcggc
      481 atcgtgctgg cgtttttatc cgatacagcg ttcagttata gcggtaactg gcgcgcaatg
      541 ttgggggttc ttgctttacc agcagttctg ctgattattc tggtagtctt cctgccaaat
      601 agcccgcgct ggctggcgga aaaggggcgt catattgagg cggaagaagt attgcgtatg
      661 ctgcgcgata cgtcggaaaa agcgcgagaa gaactcaacg aaattcgtga aagcctgaag
      721 ttaaaacagg gcggttgggc actgtttaag atcaaccgta acgtccgtcg tgctgtgttt
      781 ctcggtatgt tgttgcaggc gatgcagcag tttaccggta tgaacatcat catgtactac
      841 gcgccgcgta tcttcaaaat ggcgggcttt acgaccacag aacaacagat gattgcgact
      901 ctggtcgtag ggctgacctt tatgttcgcc acctttattg cggtgtttac ggtagataaa
      961 gcagggcgta aaccggctct gaaaattggt ttcagcgtga tggcgttagg cactctggtg
     1021 ctgggctatt gcctgatgca gtttgataac ggtacggctt ccagtggctt gtcctggctc
     1081 tctgttggca tgacgatgat gtgtattgcc ggttatgcga tgagcgccgc gccagtggtg
     1141 tggatcctgt gctctgaaat tcagccgctg aaatgccgcg atttcggtat tacctgttcg
     1201 accaccacga actgggtgtc gaatatgatt atcggcgcga ccttcctgac actgcttgat
     1261 agcattggcg ctgccggtac gttctggctc tacactgcgc tgaacattgc gtttgtgggc
     1321 attactttct ggctcattcc ggaaaccaaa aatgtcacgc tggaacatat cgaacgcaaa
     1381 ctgatggcag gcgagaagtt gagaaatatc ggcgtctgat aagaattctt cagccaaaaa
     1441 acttaagacc gccggtcttg tccactacct tgcagtaatg cggtggacag gatcggcggt
     1501 tttcttttct cttctcaacc taggctacag ccgatagtct ggaacagcgc acttacggga
     1561 cctagggcga tgcaggtggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
     1621 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
     1681 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
     1741 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
     1801 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
     1861 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
     1921 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
     1981 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
     2041 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
     2101 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
     2161 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
     2221 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
     2281 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
     2341 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
     2401 aacgcggcct gcttggcttc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
     2461 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
     2521 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
     2581 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggactat cgagccgacg
     2641 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
     2701 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
     2761 ttcattcacc ctccttgcgg gattgccccg gaattaattc cccggatcga tccgtcgatc
     2821 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
     2881 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata
     2941 aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg
     3001 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     3061 gtttctgcgg actggctttc tacgtggctg ccatttttgg ggtgaggccg ttcgcggccg
     3121 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
     3181 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
     3241 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
     3301 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
     3361 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
     3421 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
     3481 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
     3541 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
     3601 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
     3661 tccgcgaggt atccacaacg ccggcggccc tacatggctc tgctgtagtg agtgggttgc
     3721 gctccggcag cggtcctgat cccccgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3781 ttttctacac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3841 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3901 gagctcacct tcgggagcgc ctgaagcccg ttctggacgc cctggggccg ttgaatcggg
     3961 atatgcaggc caaggccgcc gcgatcatca aggccgtggg cgaaaagctg ctgacggaac
     4021 agcgggaagt ccagcgccag aaacaggccc agcgccagca ggaacgcggg cgcgcacatt
     4081 tccccgaaaa gtgccacctg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     4141 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     4201 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     4261 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     4321 tgagcgcggt ggcaatgccc tgcctccggt gctctccgga gactgcgaga tcatagatat
     4381 agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa
     4441 ccacttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga
     4501 ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga
     4561 aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa
     4621 tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat
     4681 cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     4741 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     4801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     4861 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     4921 gttcgtgcct tcatccgttt ccacggtgtg cgtccatggg caaatattat acgcaaggcg
     4981 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     5041 gcttcccaac cttaccagag ggcgccccag ctggcaattc cgacgtcctc ggtaccaaat
     5101 tccagaaaag aggcctcccg aaaggggggc cttttttcgt tttggtccat cttgaactag
     5161 catacatatt tacagctagc tcagtcctag ggactgtgct agcacgtcga cttatctcga
     5221 gtgagatatt gttgacgaac tcataaagtc aagtaggagg ttaattcc
//
