LOCUS       sequence-212729-        4075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express ELANE-cleaved mouse GSDMD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Kambara H, Liu F, Zhang X, Liu P, Bajrami B, Teng Y, Zhao L, Zhou S,
            Yu H, Zhou W, Silberstein LE, Cheng T, Han M, Xu Y, Luo HR
  TITLE     Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil
            Death.
  JOURNAL   Cell Rep. 2018 Mar 13;22(11):2924-2936. doi: 
            10.1016/j.celrep.2018.02.067.
  PUBMED    29539421
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.02"; journalName: "Cell Rep"; date: 
            "2018-03-13- 13"; volume: "22"; issue: "11"; pages: "2924-2936"
COMMENT     Sequence Label: pCAG-mGSDMD1-251
FEATURES             Location/Qualifiers
     source          1..4075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             838..861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             868..891
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             928..951
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             967..996
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1012..1029
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1073..1090)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(1100..1119)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1165..1220
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1219..1238)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1581..1597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1581..1597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1594..1616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1605..1621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1629..1635,1636..1653,1654..1659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1674..1695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1754..1949
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1800..1935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1955..2089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1992..2011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2046..2065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2157..2174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2328..2916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2408..2427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3087..3878,3879..3947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3710..3729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3948..4052)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccggaggtac ccgggcggcc gcgagtcttc gaattcaaac gcgtctcgag caccatgcca
       61 tcggcctttg agaaagtggt caagaatgtg atcaaggagg taagcggcag cagaggcgat
      121 ctcattccgg tggacagcct gcggaactcc accagcttca ggccctactg ccttctgaac
      181 aggaaatttt caagctcaag gttctggaaa ccccgttatt catgtgtcaa cctgtcaatc
      241 aaggacatcc tggagcccag tgctccagaa ccagaaccgg agtgttttgg ctccttcaaa
      301 gtctctgatg tcgtcgatgg gaacattcag ggcagagtga tgttgtcagg catgggagaa
      361 gggaaaattt ctggtggggc tgcagtgtct gacagttcca gtgcctccat gaatgtgtgt
      421 atactgcgtg tgactcagaa gacctgggag accatgcagc atgaaaggca ccttcagcag
      481 cctgagaaca aaatcctgca acagcttcgg agtcgtgggg atgacctgtt tgtggtgacc
      541 gaggtgctgc agacaaagga ggaagtgcag atcactgagg tccacagcca agagggctca
      601 ggccagttta cactgcctgg agctttatgc ttgaagggtg aaggcaaggg ccaccaaagc
      661 cggaagaaga tggtgaccat tcctgcaggc agcatcctgg cattccgagt ggcccaactg
      721 cttattggct ctaaatggga tatccttctc gtctcagatg agaaacagag gacctttgag
      781 ccctcctcag gtgacagaaa agcagtgctc gagaagcttg ctagcagatc tggattcgat
      841 tacaaggatg acgatgacaa gagcgcttgg agccacccgc agttcgagaa aggtggaggt
      901 tccggaggtg gatcgggagg tggatcgtgg agccacccgc agttcgaaaa ataaaagctt
      961 gggcccgaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga ccatcatcat
     1021 catcatcatt gagtttaaac ggtctccagc ttaagtttaa accgctgatc agcctcgact
     1081 gtgccttcta gttgccggat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     1141 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     1201 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     1261 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     1321 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     1381 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     1441 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     1501 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     1561 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1741 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     1801 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     1861 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     1921 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     2101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2761 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2881 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2941 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3001 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3061 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3121 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3181 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3241 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3961 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4021 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
