LOCUS       sequence-212852-        5050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An entry vector with a lyzC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q
  TITLE     Neutrophil-specific knockout demonstrates a role for mitochondria in
            regulating neutrophil motility in zebrafish.
  JOURNAL   Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 
            10.1242/dmm.033027.
  PUBMED    29590639
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2018-03-28"; volume: "11(3). pii: dmm.033027. doi"; 
            pages: " 10.1242/dmm.033027"
COMMENT     Sequence Label: p5E-lyzC
FEATURES             Location/Qualifiers
     source          1..5050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3092..3215
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3291..3310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3292..3310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3315..3331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3315..3331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3433..3452)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3444..4253
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3511..3530)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4400..4988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4889..4908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggc tccgaattcg cccttaaaac tcgagagttt
      721 gacttgggtt gattatagca aatagtggaa cacaaacaga ttttgaaata ttcttgcaaa
      781 tttatttatt aaatccattc aaatattgtc attttttgta ctgtgtaatt tatattgatt
      841 tgctcataca atatttacat ttcgcttaaa caatacacta ttatttatat ttaaaaatag
      901 ctatatataa atcaaatgta tagctctttt ttacacaatt aatcaaagct aaaatatttg
      961 tcacacaaat acagtaagcc acttcacaat gatatcttca aaatctgtgc ttgactgaat
     1021 catttcattt attgttttgt aattcacatt ataccttacg ttctccaggc acagtttgaa
     1081 ttgttgataa atgaagataa tacatctgat ttaattagtc tcctttgttt ataataactt
     1141 taatctaatt tgatagtaat aattccagca atacatccat tacaataaaa atcgcttttt
     1201 tattttgcac attagtttgc cggtatttgt ttaaagtttc accatacttt taaatcatgt
     1261 gctatttgaa aagggctgtt atgactggaa ttaaatgttt ttttttatat cagtgtttta
     1321 gattgaagta caatatttat aggtaaaatt caccagcata tgcttaaaga tagccatgct
     1381 ccaggtcctt gtgtccttct aaggtctgtt tgtggtttaa gctgtttgtt gacacacgct
     1441 tgtcttctcc atttttctgt gaagttattg tcagtttatt gcacactggg ttgcattaag
     1501 ctgcagtttt ttcacaacag ttttccacat tgtaacagtc agtctccaaa ttattccatc
     1561 agtcctcaaa tttagtcatt tgaggccatg aaaacactta aaactacatg aaggtagttt
     1621 ggcattaggt tttagacttg ttcttgtttg gcatattgca acctgtgtaa aaatgatttg
     1681 atttttattt tttttttttt tttttcagaa agaaacggtc caaaaagagt tatgtgaaca
     1741 tttatccatg tgtttaatgt tttttaaatt aaaaaaaaac atttttatta aaccacttgt
     1801 gactaaacct tttttagtta ttctattttg tgacgagtct gaatggccaa agtactcttg
     1861 aatccaaaat ctttatagta aaatgtttaa tcttacttgc agtgttgggt ataatttgta
     1921 acaaagttga attccatatc tgctgagtga aaaagtaaag taagagattg ctgtaaatat
     1981 ttaggttatt tatttattaa tacaatatag aaagtaacaa aacacaatgt aaaaaatcaa
     2041 taaagaaagt aaatattaaa agtaatgata aactaatgat gtaaacaata ttagcaacaa
     2101 agtttgtaca attcagggag ggtaaagaat ttcacctaat agtcaaactc tgtattaaat
     2161 aatataaaat ctttgttctt agtctgaaga ggagactgtg agagaaagct tactggttgt
     2221 taaattagaa aatttgtaac ttgcagatta aaatgattta aataatttta taaaggccat
     2281 gtttgaaaca aatgtaaaaa cataataaat gttaagaatt tcagatattg agattttaca
     2341 atttaaaaaa agcaataaag taatagcaga gacctacact cttaaagtaa attacccaac
     2401 tctgcttact agtaactttt ggaatagtta ctagtatcca ttgaacaaat actagaatct
     2461 aattaaattg tgtgagtgtc caatgaaaag attcttgtat ttaaaacagt accagtatct
     2521 atatatgtac aaaatttagt gctagcaaaa tgcaaataaa tactactgca tataatggat
     2581 tgcattgtaa atcgactcgc cataatacat ttatttccta tttttacttt taacaaaaaa
     2641 taagttcact gagtatttca tgtcattaaa tcgcattaaa cctcttgcat gctttatgta
     2701 cactagtgca gttcgctaaa tgaccaccgg tgtcactaat aacacgatcg tttctgaatt
     2761 ctgcattaaa cgccatttat acgttgctct tattagtaaa caatttctcc atcatgagct
     2821 cgaccagtat tgcatatttc atcctcttaa atccagagtt gtttctttta atcactttct
     2881 cttcatgata aaagcagttt ttggttttca gaagtttaca acaaacccag tcagttattg
     2941 cctcagcaaa cactggagca actgcacact tgtgaaaagc ttcttgcgaa acacaaacct
     3001 ttctctggac ttataaataa aggcattcag cagctccacc atacacctcc actggtcttg
     3061 ataaagggat cctttaaggg cgaattcgac ccaagtttgt acaaaaaagt tgaacgagaa
     3121 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     3181 taatactgta aaacacaaca tatgcagtca ctatgaacca actacttaga tggtattagt
     3241 gacctgtaga attaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg
     3301 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     3361 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     3421 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     3481 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     3541 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     3601 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     3661 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     3721 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     3781 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     3841 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     3901 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     3961 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     4021 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     4081 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     4141 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     4201 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     4261 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     4321 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     4381 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4441 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4501 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4741 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4801 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4861 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4921 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4981 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5041 ctcacatgtt
//
