LOCUS       sequence-212892-        1850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For tetracycline-inducible expression of myc-BirA*-NES in mammalian 
            cells. For BioID studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
  TITLE     RNA-binding proteins and heat-shock protein 90 are constituents of 
            the cytoplasmic capping enzyme interactome.
  JOURNAL   J Biol Chem. 2018 Oct 26;293(43):16596-16607. doi: 
            10.1074/jbc.RA118.004973. Epub 2018 Aug 30.
  PUBMED    30166341
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004973"; journalName: "J Biol Chem"; date: 
            "2018-10-26- 26"; volume: "293"; issue: "43"; pages: "16596-16607"
COMMENT     Sequence Label: pcDNA3.1/TO-myc-BirA*-NES
FEATURES             Location/Qualifiers
     source          1..1850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        160..363
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     314..334
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    386..404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     448..467
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        448..466
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      484..493
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             493..522
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(526..873,874..876,877..1485)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1498..1524
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(1643..1660)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1649..1760
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
       61 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      121 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      181 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      241 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      301 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      361 gctctcccta tcagtgatag agatctccct atcagtgata gagatctggc taactagaga
      421 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      481 ctagccacca tggaacaaaa actcatctca gaagaggatc tcgacaagga caacaccgtg
      541 cccctgaagc tgatcgccct gctggccaac ggcgagttcc actctggcga gcagctggga
      601 gagaccctgg gaatgagcag agccgccatc aacaagcaca tccagacact gagagactgg
      661 ggagtggacg tgttcaccgt gcctggcaag ggctacagcc tgcctgagcc tatccagctg
      721 ctgaacgcca agcagatcct gggacagctg gatggcggaa gcgtggccgt gctgcctgtg
      781 atcgactcca ccaatcagta cctgctggac agaatcggag agctgaagtc cggcgacgcc
      841 tgcatcgccg agtaccagca ggctggcaga ggaggcagag gacggaagtg gttcagccca
      901 ttcggagcca acctgtacct gtccatgttc tggagactgg agcagggacc tgctgctgcc
      961 atcggactga gtctggtgat cggaatcgtg atggccgagg tgctgagaaa gctgggagcc
     1021 gacaaggtga gagtgaagtg gcctaatgac ctgtacctcc aggaccgcaa gctggctggc
     1081 atcctggtgg agctgacagg caagacaggc gatgccgctc agatcgtgat cggagccgga
     1141 atcaacatgg ccatgagaag agtggaggag agcgtggtga accagggctg gatcaccctg
     1201 caggaggctg gcatcaacct ggaccggaac accctggccg ccatgctgat cagagagctg
     1261 agagccgctc tggagctgtt cgagcaggag ggactggctc cttacctgag cagatgggag
     1321 aagctggaca acttcatcaa cagacctgtg aagctgatca tcggcgacaa ggaaatcttc
     1381 ggcatctcca gaggaatcga caagcaggga gctctgctgc tggagcagga cggaatcatc
     1441 aagccctgga tgggcggaga aatctccctg agaagcgcag agaagctcga gcttcagcta
     1501 ccaccgcttg agagacttac tcttgatatg gccatggagg cctgatgacg gccgccactg
     1561 tgctggatat ctgcagaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc
     1621 ttaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1681 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1741 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1801 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
//
