LOCUS       sequence-212904-       12929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation and expression of Csy4 and cogGFP proteins in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Liang Y, Richardson S, Yan J, Benites VT, Cheng-Yue C, Tran T, 
            Mortimer J, Mukhopadhyay A, Keasling JD, Scheller HV, Loque D
  TITLE     Endoribonuclease-Based Two-Component Repressor Systems for Tight 
            Gene Expression Control in Plants.
  JOURNAL   ACS Synth Biol. 2017 May 19;6(5):806-816. doi: 
            10.1021/acssynbio.6b00295. Epub 2017 Feb 8.
  PUBMED    28094975
REFERENCE   2  (bases 1 to 12929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00295"; journalName: "ACS Synth Biol"; date: 
            "2017-05-19- 19"; volume: "6"; issue: "5"; pages: "806-816"
COMMENT     Sequence Label: pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
FEATURES             Location/Qualifiers
     source          1..12929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    183..204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(219..224,225..242,243..249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    257..273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     262..284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     281..297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     281..297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        600..945
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     888..908
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    976..1000
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1009..1029
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1601..1621)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      1645..1845
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        1991..2170
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    2217..2237
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2241..2260
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             join(2270..2272,2273..2275,2276..2986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2315..2336)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2576..2595)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2923..2944
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2988..3012)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3110..3751
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3759..3776)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3759..3775)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3768..3790)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(4357..4609)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4805..5599)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4916..4935)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5526..5545
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5629..6009)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    6381..6405
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6926..7717
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7963..8551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8452..8471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8705..8722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8737..8877
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8803..8822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8963..8985
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9221..9415
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9481..10554)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10983..11612)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    join(12923..12929,1..18)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
       61 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      301 attacgaatt cgagctcggt acccggggat cctctagagg gcccgggacg tccgcggaga
      361 tctacgcgtg tcgactcgag cggccgccag tgtgatggat atctgcagaa ttcaggcatg
      421 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc
      481 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac
      541 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
      601 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
      661 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
      721 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
      781 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
      841 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
      901 gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg gactcctagg
      961 gctagcctgt tatcaacaag tttgtacaaa aaagcaggct tcatggctcc aaaaaagaaa
     1021 agaaaggttg ctagcgacca ttacttagat attagattga gaccagaccc agagttccct
     1081 ccagcacagt tgatgtcagt gttattcgga aaattgcacc aagctttggt tgcacaaggt
     1141 ggagatagaa ttggtgtgag ttttcctgat ttggacgagt ctaggtcaag attgggagaa
     1201 aggttaagaa tccatgcttc agcagatgac cttagggctt tgttagcaag accttggtta
     1261 gagggtctta gagatcattt gcagttcgga gaaccagctg ttgtgccaca ccctacacca
     1321 tatagacaag tttctagagt gcaggcaaaa tcaaatcctg agagacttag aaggagattg
     1381 atgaggagac acgacttatc tgaagaggaa gctaggaaga gaatacctga taccgttgct
     1441 agagcacttg acttgccatt tgtgactttg agaagtcaat ccactggtca gcattttagg
     1501 ttattcatta gacacggacc acttcaagtt actgcagagg agggaggatt cacctgttat
     1561 ggattgagta agggaggatt cgtgccttgg ttctgacacc caacttttct atacaaagtt
     1621 gactgactaa ctaggatgag ctaagctagc tatatcatca atttatgtat tacacataat
     1681 atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt tattgaaata
     1741 tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat aatacctgac
     1801 ttgttatttt atcaataaat atttaaacta tatttctttc aagatgggaa ttaacatcta
     1861 caaattgcct tttcttatcg accatgtacc ccgggtacca agcttctcga ggatacatga
     1921 gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt
     1981 ggaactgaca gaaccgcaac gttgaaggag ccactgagcc gcgggtttct ggagtttaat
     2041 gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta
     2101 tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg
     2161 tatccaatta gagtctcata ttcactctca actcgatcga ggggatctac ctaggacaac
     2221 tttgtataat aaagttggcc gttcactgcc gtataggcag ctaagaaata tggtgagcaa
     2281 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2341 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     2401 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2461 cttcacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     2521 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     2581 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     2641 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2701 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     2761 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     2821 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     2881 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     2941 cgtgaccgcc gccgggatca ctcacggcat ggacgagctg tacaaggacc cagctttctt
     3001 gtacaaagtg gttgataaca gcgctgagct ctagcacgtg cgggcctgaa ttccagcaca
     3061 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttgatgcaga gctttcgttc
     3121 gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac
     3181 cagaatccta ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     3241 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     3301 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     3361 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     3421 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     3481 agtaaaacac ttgtagttgt accattatgc ttattcacta ggcaacaaat atattttcag
     3541 acctagaaaa gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt ttagacattt
     3601 atgaactttc ctttatgtaa ttttccagaa tccttgtcag attctaatca ttgctttata
     3661 attatagtta tactcatgga tttgtagttg agtatgaaaa tattttttaa tgcattttat
     3721 gacttgccaa ttgattgaca acatgcatca agcttggcac tggccgtcgt tttacaacgt
     3781 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3841 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3901 ctgaatggcg aatgctagag cagcttgcca atcgaggccg accaaccgca agcgttgtca
     3961 gtgttgcaaa gcgctctgtg tgggcctact ttaattgctt ccagtgttaa attggcgaaa
     4021 ggcaataata tcgcaaaata ttgtgttgta aaatgtaatt atgttttaat ttcatggaaa
     4081 tgtttgagca taatttttat taatgtacta aattactgtt ttgttaaatg caattttgct
     4141 ttctcgggat tttaatatca aaatctattt agaaatacac aatattttgt tgcaggcttg
     4201 ctggagaatc gatctgctat cataaaaatt acaaaaaaat tttatttgcc tcaattattt
     4261 taggattggt attaaggacg cttaaattat ttgtcgggtc actacgcatc attgtgattg
     4321 agaagatcag cgatacgaaa tattcgtagt actatcgata atttatttga aaattcataa
     4381 gaaaagcaaa cgttacatga attgatgaaa caatacaaag acagataaag ccacgcacat
     4441 ttaggatatt ggccgagatt actgaatatt gagtaagatc acggaatttc tgacaggagc
     4501 atgtcttcaa ttcagcccaa atggcagttg aaatactcaa accgccccat atgcaggagc
     4561 ggatcattca ttgtttgttt ggttgccttt gccaacatgg gagtccaagg tttcagggaa
     4621 gctggaattc cggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     4681 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     4741 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     4801 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     4861 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     4921 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     4981 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     5041 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     5101 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     5161 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     5221 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     5281 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     5341 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     5401 gccgcgctgc ctcgtcctgg agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     5461 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     5521 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     5581 atccatcttg ttcaatcatg cgaaacgatc cggggaattc gctagagtcg atttggtgta
     5641 tcgagattgg ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata
     5701 ataggaagca aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc
     5761 acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg
     5821 gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat
     5881 agaaacagaa tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac
     5941 gaccaaatta gtagaaaaat aaaaactgac tcggatactt acgtcacgtc ttgcgcactg
     6001 atttgaaaaa tctcaatata aacaaagacg gccacaagaa aaaaccaaaa caccgatatt
     6061 cattaatctt atctagtttc tcaaaaaaat tcatatcttc cacacgtgaa aatgccaatt
     6121 tctcagacct acctcggctc tgcgaaggcc cccgctggta tcaaaagttt ttatttcatc
     6181 cgacatggcg cgaccgacct caacgagaag gaaattgtcg tgaacggtga gaagctctgg
     6241 ggcgtgcaag gttccggaac gaacatcggt ctcaatgcaa aaggggaacg ccaggctctg
     6301 ttggcccctc gaaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
     6361 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     6421 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
     6481 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
     6541 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
     6601 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
     6661 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     6721 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     6781 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     6841 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     6901 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
     6961 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     7021 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     7081 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     7141 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     7201 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     7261 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     7321 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     7381 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     7441 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     7501 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     7561 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     7621 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     7681 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     7741 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     7801 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     7861 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     7921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8101 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8581 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8641 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8701 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     8761 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     8821 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     8881 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8941 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     9001 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     9061 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     9121 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     9181 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     9241 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     9301 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     9361 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     9421 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     9481 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     9541 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     9601 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     9661 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     9721 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     9781 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     9841 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     9901 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     9961 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
    10021 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
    10081 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
    10141 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
    10201 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
    10261 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
    10321 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
    10381 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
    10441 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
    10501 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat
    10561 tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg
    10621 cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt
    10681 tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt
    10741 gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc
    10801 ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac
    10861 ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc
    10921 gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta
    10981 actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg
    11041 ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg
    11101 cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc
    11161 tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt
    11221 ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg
    11281 tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca
    11341 gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc
    11401 ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc
    11461 cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg
    11521 gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg
    11581 cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta
    11641 ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc
    11701 acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca
    11761 gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg
    11821 gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa
    11881 acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc
    11941 ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct
    12001 ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg
    12061 cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg
    12121 ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc
    12181 cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc
    12241 aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg
    12301 ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag
    12361 gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg
    12421 taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc
    12481 ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg
    12541 gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc
    12601 gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag
    12661 aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat
    12721 gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag
    12781 gggttggatc aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12841 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    12901 aataaacgct cttttctctt aggtttacc
//
