LOCUS       sequence-212982-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PCDH7 transcript variant c in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Zhou X, Updegraff BL, Guo Y, Peyton M, Girard L, Larsen JE, Xie XJ, 
            Zhou Y, Hwang TH, Xie Y, Rodriguez-Canales J, Villalobos P, Behrens 
            C, Wistuba II, Minna JD, O'Donnell KA
  TITLE     PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK 
            Signaling by EGFR and KRAS during Lung Tumorigenesis.
  JOURNAL   Cancer Res. 2017 Jan 1;77(1):187-197. doi: 
            10.1158/0008-5472.CAN-16-1267-T. Epub 2016 Nov 7.
  PUBMED    27821484
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-16-1267-T"; journalName: "Cancer Res"; date: 
            "2017-01-1- 1"; volume: "77"; issue: "1"; pages: "187-197"
COMMENT     Sequence Label: pLX304-PCDH7-c
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4395..4419)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4421..4462
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4504..5092
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4557..4577)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4975..4986)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5164..5397
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5475..5596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5512..5531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5566..5585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5636..5771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5791..5810)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5792..5810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5820..5837)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5820..5836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5829..5851)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5978..6433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6065..6084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6275..6296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6459..6563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6564..6632,6633..7424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6782..6801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7595..8183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8084..8103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8337..8354
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8471..8492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8507..8512,8513..8530,8531..8537)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8545..8561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8550..8572
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8569..8585
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8569..8585
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8604..8624
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8606..8624
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8652..8878
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8879..9059
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9106..9231
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9724..9957
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10142..10186
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10361..10861
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10780..10798
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10873..11268
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11328..11445
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctc cgaattcgcc cttcaccatg ctgaggatgc ggaccgcggg
      661 atgggcgcgc ggctggtgct tgggctgctg cctcctcctg ccgctctcgc tcagcctggc
      721 ggccgccaag cagctcctcc ggtaccggct ggccgaggag ggccccgccg acgtccgcat
      781 cggcaacgtg gcttcagacc tgggcatcgt gaccggatcg ggtgaggtga ctttcagcct
      841 ggagtccggt tccgagtacc tgaagatcga caacctcact ggcgagctga gcacgagcga
      901 gcggcgcatc gaccgcgaga agctgcccca gtgtcagatg atcttcgacg agaacgagtg
      961 cttcctggac ttcgaggtgt cggtgatcgg gccctcgcag agctgggtgg acctgtttga
     1021 gggtcaggtc atcgtgcttg acatcaacga caacacgccc accttcccgt cgcccgtgct
     1081 cacgctcacg gtggaggaga atcggccggt gggcacactt tacctgctgc ccacagccac
     1141 cgaccgcgac ttcggccgca acggcatcga gcgctacgag ctgctccagg agcccggagg
     1201 cggcggcagc ggcggcgaga gccggcgcgc cggggcggcc gacagcgccc cctaccccgg
     1261 gggcggcggg aacggcgcga gcggcggcgg ctcgggaggc tccaagcggc ggctggacgc
     1321 atcagagggc ggcggcggca ccaaccccgg cggccgcagc agcgtgttcg agctgcaggt
     1381 ggcggacacc ccggacggcg agaagcagcc gcagctgatc gtgaaggggg cgctggaccg
     1441 cgagcagcgc gactcctacg agctgaccct gcgagtgcgc gacggcggcg acccgcctcg
     1501 ctcctcgcag gccatcctac gggtcctcat caccgacgtg aacgacaaca gcccccgctt
     1561 cgagaagagc gtgtacgagg ccgacttggc tgagaacagc gccccgggga cccccatcct
     1621 gcaactgcgc gcagccgact tggacgtggg ggtcaacggg cagatcgaat acgtgttcgg
     1681 ggcggccacc gagtcggtga ggcggctgct gcgccttgac gagacgtccg gctggctcag
     1741 cgtcctgcac cggatcgacc gcgaggaggt gaaccagctg cgcttcacgg tcatggcccg
     1801 cgaccgcggg cagcccccca agaccgacaa ggccaccgtg gtccttaaca tcaaagacga
     1861 gaacgacaac gtgccgtcca ttgaaatccg caagattggg cgcatccccc tcaaggacgg
     1921 ggtggccaac gtggccgagg acgttctggt cgacaccccc atcgctctgg tgcaggtgtc
     1981 cgaccgagac caaggcgaga acggggtggt cacctgcacc gtggtgggcg acgtgccctt
     2041 ccagctcaag ccagccagcg acaccgaggg cgaccagaac aagaaaaagt acttcttgca
     2101 cacctcgacc cctctggact atgaggccac ccgggagttc aacgtggtca tcgtggcggt
     2161 ggactcaggc agccccagcc tctcgagcaa caactccctg attgtcaagg tgggagacac
     2221 caacgacaac ccgcccatgt tcggccagtc ggtggtggag gtttacttcc ctgagaacaa
     2281 catcccgggc gagagggtgg ccacggtgct ggcgacagac gcagacagcg gtaagaacgc
     2341 cgagatcgcc tactcgctgg actcctctgt gatggggatc tttgccatcg atcccgattc
     2401 tggggacatc ctggtcaata ccgtgctgga ccgcgagcag actgacaggt atgagtttaa
     2461 agttaacgcc aaagacaaag gcatccccgt gctgcagggc agcactacgg tgattgtgca
     2521 ggtggctgat aaaaatgaca atgaccctaa gtttatgcag gacgtcttca ccttttatgt
     2581 gaaagaaaac ttgcagccca acagccctgt ggggatggtc accgtgatgg atgctgacaa
     2641 ggggcggaat gcagagatga gcctgtacat agaggagaac aataacattt tttctattga
     2701 aaatgacacg gggaccattt actccacaat gtcttttgac cgggaacatc agaccacata
     2761 cactttcaga gtcaaggctg tggatggggg agatcctccc agatctgcca cagctacagt
     2821 ctcgcttttt gtgatggatg aaaatgacaa tgctcccaca gttacccttc ccaaaaacat
     2881 ttcctacact ttactgccac cttcgagtaa tgtcaggaca gtagtagcta cagtgttggc
     2941 aacagacagt gatgatggca tcaatgcaga cctgaactac agcattgtgg gaggaaatcc
     3001 cttcaagctg tttgaaattg atcccactag tggtgtggtt tccttagtgg gaaaactcac
     3061 ccaaaagcat tatggcttgc acaggttggt ggtgcaagtg aatgacagtg ggcagccttc
     3121 ccagtccacc acgactctgg tgcacgtgtt tgtcaatgaa agtgtttcta atgcaactgc
     3181 gattgactcc cagatagcta gaagtttgca catcccactc acccaggata tagctggtga
     3241 cccaagctat gaaattagca aacagagact cagtattgtc attggcgtgg ttgctggcat
     3301 tatgacggtg attctaatca tcttaattgt agtgatggca aggtactgca ggtccaaaaa
     3361 taaaaatggc tatgaagccg gcaaaaaaga tcacgaagac ttttttacac cccaacagca
     3421 tgacaaatct aaaaagccta aaaaggacaa gaaaaacaaa aaatctaagc agcctctcta
     3481 cagcagcatt gtcactgtgg aggcttctaa gccaaatgga cagaggtatg atagtgtcaa
     3541 tgagaagctg tcagacagcc caagcatggg gcgatacagg tccgttaatg gtgggcccgg
     3601 cagtcctgac ctggcaaggc attacaaatc tagttcccca ttgcctactg ttcagcttca
     3661 tccccagtca ccaactgcag gaaaaaaaca ccaggccgta caagatctac caccagccaa
     3721 cacatttgtg ggagcaggag acaacatttc aattggatca gatcactgct ctgagtacag
     3781 ctgtcaaacc aataacaagt acagcaaaca gccatttcgt agagtgacgt tttctgttgt
     3841 gagtcagcct caggacccac atcaggggtc actgcagagt tgctatgaca gcgggctgga
     3901 ggagtcagaa acaccaagca gtaagagttc atcagggcca agactgggtg cgcttccact
     3961 cccagaggac aactatgaaa ggaccacgcc ggatggcagt gttgattcaa ggcctcttcc
     4021 agatgtagcc ctgactggga agtgcactcg tgagtgtgat gagtatggcc actcagactc
     4081 ctgctggatg ccggtccgca cttctccgga gaggaagaag agccagccta aactgtccac
     4141 tttcatgcct gttgatgaac gaggaagcca ggaaaagctg gccaatgggg aggccgccat
     4201 catgggtgac cgcaacagaa acctcctgaa caaaaagttg acctcatcct atgagacctt
     4261 cagtgcagct agtttcagca aaaatgagga agccaaccct gaggatattc cccttacaaa
     4321 aacaggggaa tataagccat ctcctgtcaa tactctcact agaagagaag tttacctgta
     4381 gaagggcgaa ttcgacccag ctttcttgta caaagtggtt ggtaagccta tccctaaccc
     4441 tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc
     4501 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4561 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4621 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4681 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4741 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4801 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4861 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4921 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4981 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5041 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     5101 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5161 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5401 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5461 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5581 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5641 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5701 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5761 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     5821 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5881 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5941 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6421 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6481 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6541 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6601 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6661 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6721 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6781 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6841 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6901 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6961 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7021 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7081 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7141 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7201 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7261 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7321 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7381 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7441 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7501 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8461 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8521 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8581 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8641 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     8701 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     8761 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     8821 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     8881 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8941 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9001 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9061 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9121 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9181 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9241 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9301 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9361 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9421 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9481 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9541 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9901 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     9961 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10021 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10081 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10141 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10201 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10261 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10321 attatcgttt cagacccacc tcccaacccc gaggggaccc ttgcgccttt tccaaggcag
    10381 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
    10441 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
    10501 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
    10561 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
    10621 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
    10681 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
    10741 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
    10801 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
    10861 gggggtacca ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
    10921 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
    10981 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
    11041 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
    11101 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
    11161 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
    11221 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcct gcagctgcag
    11281 tagtaagaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
    11341 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    11401 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    11461 gacagcagag atccactttg gcgccggctc gaggggg
//
