LOCUS       sequence-212989-       11484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PCDH7 transcript variant d in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Zhou X, Updegraff BL, Guo Y, Peyton M, Girard L, Larsen JE, Xie XJ, 
            Zhou Y, Hwang TH, Xie Y, Rodriguez-Canales J, Villalobos P, Behrens 
            C, Wistuba II, Minna JD, O'Donnell KA
  TITLE     PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK 
            Signaling by EGFR and KRAS during Lung Tumorigenesis.
  JOURNAL   Cancer Res. 2017 Jan 1;77(1):187-197. doi: 
            10.1158/0008-5472.CAN-16-1267-T. Epub 2016 Nov 7.
  PUBMED    27821484
REFERENCE   2  (bases 1 to 11484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-16-1267-T"; journalName: "Cancer Res"; date: 
            "2017-01-1- 1"; volume: "77"; issue: "1"; pages: "187-197"
COMMENT     Sequence Label: pLX303-PCDH7-d
FEATURES             Location/Qualifiers
     source          1..11484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4424..4448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4491..5079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4544..4564)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4962..4973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5151..5384
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5462..5583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5499..5518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5553..5572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5623..5758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5778..5797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5779..5797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5807..5824)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5807..5823)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5816..5838)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5965..6420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6052..6071)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6262..6283
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6446..6550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6551..6619,6620..7411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6769..6788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8324..8341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8494..8499,8500..8517,8518..8524)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8532..8548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8537..8559
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8556..8572
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8556..8572
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8591..8611
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8593..8611
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8639..8865
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8866..9046
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9093..9218
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9711..9944
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10129..10173
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10348..10848
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10767..10785
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10860..11255
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11315..11432
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tgctgaggat gcggaccgcg
      661 ggatgggcgc gcggctggtg cttgggctgc tgcctcctcc tgccgctctc gctcagcctg
      721 gcggccgcca agcagctcct ccggtaccgg ctggccgagg agggccccgc cgacgtccgc
      781 atcggcaacg tggcttcaga cctgggcatc gtgaccggat cgggtgaggt gactttcagc
      841 ctggagtccg gttccgagta cctgaagatc gacaacctca ctggcgagct gagcacgagc
      901 gagcggcgca tcgaccgcga gaagctgccc cagtgtcaga tgatcttcga cgagaacgag
      961 tgcttcctgg acttcgaggt gtcggtgatc gggccctcgc agagctgggt ggacctgttt
     1021 gagggtcagg tcatcgtgct tgacatcaac gacaacacgc ccaccttccc gtcgcccgtg
     1081 ctcacgctca cggtggagga gaatcggccg gtgggcacac tttacctgct gcccacagcc
     1141 accgaccgcg acttcggccg caacggcatc gagcgctacg agctgctcca ggagcccgga
     1201 ggcggcggca gcggcggcga gagccggcgc gccggggcgg ccgacagcgc cccctacccc
     1261 gggggcggcg ggaacggcgc gagcggcggc ggctcgggag gctccaagcg gcggctggac
     1321 gcatcagagg gcggcggcgg caccaacccc ggcggccgca gcagcgtgtt cgagctgcag
     1381 gtggcggaca ccccggacgg cgagaagcag ccgcagctga tcgtgaaggg ggcgctggac
     1441 cgcgagcagc gcgactccta cgagctgacc ctgcgagtgc gcgacggcgg cgacccgcct
     1501 cgctcctcgc aggccatcct acgggtcctc atcaccgacg tgaacgacaa cagcccccgc
     1561 ttcgagaaga gcgtgtacga ggccgacttg gctgagaaca gcgccccggg gacccccatc
     1621 ctgcaactgc gcgcagccga cttggacgtg ggggtcaacg ggcagatcga atacgtgttc
     1681 ggggcggcca ccgagtcggt gaggcggctg ctgcgccttg acgagacgtc cggctggctc
     1741 agcgtcctgc accggatcga ccgcgaggag gtgaaccagc tgcgcttcac ggtcatggcc
     1801 cgcgaccgcg ggcagccccc caagaccgac aaggccaccg tggtccttaa catcaaagac
     1861 gagaacgaca acgtgccgtc cattgaaatc cgcaagattg ggcgcatccc cctcaaggac
     1921 ggggtggcca acgtggccga ggacgttctg gtcgacaccc ccatcgctct ggtgcaggtg
     1981 tccgaccgag accaaggcga gaacggggtg gtcacctgca ccgtggtggg cgacgtgccc
     2041 ttccagctca agccagccag cgacaccgag ggcgaccaga acaagaaaaa gtacttcttg
     2101 cacacctcga cccctctgga ctatgaggcc acccgggagt tcaacgtggt catcgtggcg
     2161 gtggactcag gcagccccag cctctcgagc aacaactccc tgattgtcaa ggtgggagac
     2221 accaacgaca acccgcccat gttcggccag tcggtggtgg aggtttactt ccctgagaac
     2281 aacatcccgg gcgagagggt ggccacggtg ctggcgacag acgcagacag cggtaagaac
     2341 gccgagatcg cctactcgct ggactcctct gtgatgggga tctttgccat cgatcccgat
     2401 tctggggaca tcctggtcaa taccgtgctg gaccgcgagc agactgacag gtatgagttt
     2461 aaagttaacg ccaaagacaa aggcatcccc gtgctgcagg gcagcactac ggtgattgtg
     2521 caggtggctg ataaaaatga caatgaccct aagtttatgc aggacgtctt caccttttat
     2581 gtgaaagaaa acttgcagcc caacagccct gtggggatgg tcaccgtgat ggatgctgac
     2641 aaggggcgga atgcagagat gagcctgtac atagaggaga acaataacat tttttctatt
     2701 gaaaatgaca cggggaccat ttactccaca atgtcttttg accgggaaca tcagaccaca
     2761 tacactttca gagtcaaggc tgtggatggg ggagatcctc ccagatctgc cacagctaca
     2821 gtctcgcttt ttgtgatgga tgaaaatgac aatgctccca cagttaccct tcccaaaaac
     2881 atttcctaca ctttactgcc accttcgagt aatgtcagga cagtagtagc tacagtgttg
     2941 gcaacagaca gtgatgatgg catcaatgca gacctgaact acagcattgt gggaggaaat
     3001 cccttcaagc tgtttgaaat tgatcccact agtggtgtgg tttccttagt gggaaaactc
     3061 acccaaaagc attatggctt gcacaggttg gtggtgcaag tgaatgacag tgggcagcct
     3121 tcccagtcca ccacgactct ggtgcacgtg tttgtcaatg aaagtgtttc taatgcaact
     3181 gcgattgact cccagatagc tagaagtttg cacatcccac tcacccagga tatagctggt
     3241 gacccaagct atgaaattag caaacagaga ctcagtattg tcattggcgt ggttgctggc
     3301 attatgacgg tgattctaat catcttaatt gtagtgatgg caaggtactg caggtccaaa
     3361 aataaaaatg gctatgaagc cggcaaaaaa gatcacgaag acttttttac accccaacag
     3421 catgacaaat ctaaaaagcc taaaaaggac aagaaaaaca aaaaatctaa gcagcctctc
     3481 tacagcagca ttgtcactgt ggaggcttct aagccaaatg gacagaggta tgatagtgtc
     3541 aatgagaagc tgtcagacag cccaagcatg gggcgataca ggtccgttaa tggtgggccc
     3601 ggcagtcctg acctggcaag gcattacaaa tctagttccc cattgcctac tgttcagctt
     3661 catccccagt caccaactgc aggaaaaaaa caccaggccg tacaagatct accaccagcc
     3721 aacacatttg tgggagcagg agacaacatt tcaattggat cagatcactg ctctgagtac
     3781 agctgtcaaa ccaataacaa gtacagcaaa cagccatttc gtagagtgac gttttctgtt
     3841 gtgagtcagc ctcaggaccc acatcagggg tcactgcaga gttgctatga cagcgggctg
     3901 gaggagtcag aaacaccaag cagtaagagt tcatcagggc caagactggg tgcgcttcca
     3961 ctcccagagg acaactatga aaggaccacg ccggatggca gtgttggtga ggcagagcat
     4021 atggaaaatg attcaaggcc tcttccagat gtagccctga ctgggaagtg cactcgtgag
     4081 tgtgatgagt atggccactc agactcctgc tggatgccgg tccgcacttc tccggagagg
     4141 aagaagagcc agcctaaact gtccactttc atgcctgttg atgaacgagg aagccaggaa
     4201 aagctggcca atggggaggc cgccatcatg ggtgaccgca acagaaacct cctgaacaaa
     4261 aagttgacct catcctatga gaccttcagt gcagctagtt tcagcaaaaa tgaggaagcc
     4321 aaccctgagg atattcccct tacaaaaaca ggggaatata agccatctcc tgtcaatact
     4381 ctcactagaa gagaagttta cctgtagaag ggtgggcgcg ccgacccagc tttcttgtac
     4441 aaagtggttt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc
     4501 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4561 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4621 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4681 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4741 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4801 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4861 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4921 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4981 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5041 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     5101 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5161 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5221 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5281 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5341 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     5401 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5461 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5581 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5701 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5761 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5821 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5881 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5941 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6001 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6061 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6121 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6181 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6241 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6301 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6361 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8401 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8461 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8521 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     8581 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     8641 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     8701 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     8761 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     8821 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     8881 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8941 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9001 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     9061 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     9121 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9181 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9241 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9301 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9361 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9421 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9481 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9541 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9601 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9661 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9721 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9781 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9841 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9901 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9961 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    10021 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    10081 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    10141 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    10201 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10261 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    10321 acccacctcc caaccccgag gggacccttg cgccttttcc aaggcagccc tgggtttgcg
    10381 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
    10441 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
    10501 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
    10561 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
    10621 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
    10681 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
    10741 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
    10801 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccacca
    10861 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
    10921 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
    10981 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
    11041 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
    11101 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
    11161 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
    11221 gtgaattgct gccctctggt tatgtgtggg agggcctgca gctgcagtag taagaattct
    11281 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    11341 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    11401 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    11461 cactttggcg ccggctcgag gggg
//
