LOCUS       sequence-21306-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flp recombinase-dependent expression of GFP, i.e. GFP is only 
            expressed in the presence of Flp due to a transcriptional stop in 
            between the FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCAFNF-GFP
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..57
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(154..173)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ttgattaatt cgagcgaacg cgtgaagttc ctattctcta gaaagtatag gaacttccgg
       61 atttgatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
      121 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
      181 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
      241 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
      301 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
      361 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
      421 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
      481 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
      541 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
      601 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
      661 catggcgatg cctgcttgcc ga
//
