LOCUS       sequence-213075-        3243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Delivery of human-codon-optimized Cas9 from Neisseria meningitidis 
            (NmeCas9) and its single-guide RNA in a single AAV vector for in 
            vivo genome editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ibraheim R, Song CQ, Mir A, Amrani N, Xue W, Sontheimer EJ
  TITLE     All-in-one adeno-associated virus delivery and genome editing by 
            Neisseria meningitidis Cas9 in vivo.
  JOURNAL   Genome Biol. 2018 Sep 19;19(1):137. doi: 10.1186/s13059-018-1515-0.
  PUBMED    30231914
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-09-19"; volume: "19(1):137. doi"; pages: " 
            10.1186/s13059-018-1515-0"
COMMENT     Sequence Label: pEJS654 All-in-One AAV-sgRNA-hNmeCas9
FEATURES             Location/Qualifiers
     source          1..3243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..3243
                     /codon_start=1
                     /product="Cas9 endonuclease from the Neisseria meningitidis
                     Type II CRISPR/Cas system (Esvelt et al., 2013)"
                     /label=NmCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="AAFKPNSINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVRVF
                     ERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIKS
                     LPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLKGVAG
                     NAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEFG
                     NPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTK
                     LNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKGLRYGKDN
                     AEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLK
                     DRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDHYGKKNTE
                     EKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRKE
                     IEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYSGKEINLGR
                     LNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEFK
                     ARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKRV
                     FASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRFVRYKEMNAFD
                     GKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFEEADTLEKLRTLLAEK
                     LSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDL
                     EKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKT
                     GVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQL
                     IDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGILEG
                     IGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR"
ORIGIN
        1 gcagcattca agccaaactc aatcaattac atcctgggac tggacatcgg catcgcatcc
       61 gtcgggtggg ctatggtcga aatcgacgag gaggagaacc ccatccgcct gatcgatctg
      121 ggcgtgcgcg tgtttgagag ggcagaggtg cctaagaccg gcgacagcct ggccatggca
      181 cggagactgg cacgctccgt gaggcgcctg acccggagaa gggcccacag actgctgagg
      241 acacgccggc tgctgaagag ggagggcgtg ctgcaggccg ccaacttcga tgagaatggc
      301 ctgatcaagt ccctgcccaa taccccttgg cagctgaggg cagccgccct ggaccgcaag
      361 ctgacacctc tggagtggtc cgccgtgctg ctgcacctga tcaagcaccg gggctacctg
      421 tctcagagaa agaacgaggg cgagacagcc gataaggagc tgggcgccct gctgaaggga
      481 gtggcaggaa atgcacacgc cctgcagacc ggcgactttc gcacaccagc cgagctggcc
      541 ctgaacaagt tcgagaagga gagcggccac atccgcaatc agcggtctga ctatagccac
      601 accttctccc ggaaggatct gcaggccgag ctgatcctgc tgtttgagaa gcagaaggag
      661 ttcggcaacc cacacgtgtc tggcggcctg aaggagggca tcgagacact gctgatgaca
      721 cagcggcccg ccctgagcgg cgacgcagtg cagaagatgc tgggacactg cacctttgag
      781 ccagccgagc ccaaggccgc caagaatacc tacacagccg agcggttcat ctggctgaca
      841 aagctgaaca atctgaggat cctggagcag ggaagcgagc gcccactgac cgacacagag
      901 agggccaccc tgatggatga gccctaccgc aagtccaagc tgacatatgc acaggcaagg
      961 aagctgctgg gcctggagga caccgccttc tttaagggcc tgagatacgg caaggataac
     1021 gccgaggcct ctacactgat ggagatgaag gcctatcacg ccatcagcag ggccctggag
     1081 aaggagggcc tgaaggacaa gaagtcccca ctgaatctgt ctcccgagct gcaggatgag
     1141 atcggcaccg cctttagcct gttcaagacc gacgaggata tcacaggcag actgaaggac
     1201 aggatccagc cagagatcct ggaggccctg ctgaagcaca tcagctttga taagttcgtg
     1261 cagatcagcc tgaaggccct gcggaggatc gtgccactga tggagcaggg caagaggtac
     1321 gacgaggcct gcgccgaaat ctacggcgat cactatggca agaagaacac agaggagaaa
     1381 atctacctgc cccctatccc cgccgatgag atcaggaacc ctgtggtgct gcgcgccctg
     1441 tctcaggcaa gaaaagtgat caacggagtg gtgcgccggt acggcagccc cgccagaatc
     1501 cacatcgaga cagccaggga agtgggcaag tcctttaagg acagaaagga gatcgagaag
     1561 aggcaggagg agaacagaaa ggatagggag aaggccgccg ccaagttcag agagtacttt
     1621 cctaatttcg tgggcgagcc aaagtccaag gatatcctga agctgaggct gtacgagcag
     1681 cagcacggca agtgtctgta ttctggcaag gagatcaacc tgggccgcct gaatgagaag
     1741 ggctatgtgg agatcgacca cgccctgcct ttttctcgga cctgggacga tagcttcaac
     1801 aataaggtgc tggtgctggg ctctgagaac cagaataagg gcaaccagac accctacgag
     1861 tatttcaacg gcaaggacaa tagccgcgag tggcaggagt ttaaggcaag ggtggagaca
     1921 agcaggttcc ctcggtccaa gaagcagaga atcctgctgc agaagtttga cgaggatggc
     1981 ttcaaggaga ggaacctgaa tgacacccgc tacgtgaatc ggtttctgtg ccagttcgtg
     2041 gccgatagaa tgaggctgac cggcaagggc aagaagagag tgtttgcctc caacggccag
     2101 atcacaaatc tgctgagggg cttctggggc ctgagaaagg tgagggcaga gaacgacagg
     2161 caccacgcac tggatgcagt ggtggtggca tgttctaccg tggccatgca gcagaagatc
     2221 acacgctttg tgcggtataa ggagatgaat gccttcgacg gcaagaccat cgataaggag
     2281 acaggcgagg tgctgcacca gaagacacac tttcctcagc catgggagtt ctttgcccag
     2341 gaagtgatga tccgggtgtt tggcaagcct gacggcaagc cagagttcga ggaggccgat
     2401 accctggaga agctgagaac actgctggca gagaagctga gctccaggcc cgaggcagtg
     2461 cacgagtacg tgaccccact gttcgtgtct agagccccca acaggaagat gagcggccag
     2521 ggccacatgg agacagtgaa gtccgccaag agactggacg agggcgtgtc tgtgctgagg
     2581 gtgcctctga cacagctgaa gctgaaggat ctggagaaga tggtgaatcg cgagcgggag
     2641 ccaaagctgt atgaggccct gaaggcaagg ctggaggcac acaaggacga tcctgccaag
     2701 gcctttgccg agccattcta caagtatgat aaggccggca acagaaccca gcaggtgaag
     2761 gccgtgaggg tggagcaggt gcagaagaca ggcgtgtggg tgcgcaacca caatggcatc
     2821 gccgacaatg ctaccatggt gcgggtggac gtgtttgaga agggcgataa gtactatctg
     2881 gtgcccatct acagctggca ggtggccaag ggcatcctgc ctgatagagc cgtggtgcag
     2941 ggcaaggacg aggaggattg gcagctgatc gacgattcct tcaactttaa gttctctctg
     3001 caccccaatg acctggtgga agtgatcacc aagaaggcca ggatgtttgg ctacttcgcc
     3061 tcctgccacc gcggcacagg caacatcaat atccggatcc acgacctgga tcacaagatc
     3121 ggcaagaacg gcatcctgga gggcatcggc gtgaagacag ccctgagctt ccagaagtat
     3181 cagatcgacg agctgggcaa ggagatcaga ccttgtaggc tgaagaagcg cccacccgtg
     3241 cgg
//
