LOCUS       sequence-213183-        6084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6084)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..6084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2508..2629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2545..2564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2599..2618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2636..3091)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2773..2794)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2985..3004
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3118..3222
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3224..3581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3224..3415
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3247..3267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3432..3567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3616..4410
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3616..4410
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3670..3689)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4280..4299
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4598..4617)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4642..4689
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5018..5606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5507..5526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5781..6084
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg
      301 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
      361 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
      421 ccactgcatt ccattacggc aacagggtat tcgccaaata tccagacaac atacaagact
      481 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
      541 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
      601 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
      661 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc
      721 atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
      781 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
      841 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
      901 ctggattgcc tgacaatgcc agacgatccg gactcagatc tggcagcggt ggaggcagcg
      961 catccggcgg aagcggaagc tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt
     1021 tcggcggccc gggcaccgcg agccggccga gctccagccg gagctacgtg actacgtcca
     1081 cccgcaccta cagcctgggc agcgcgctgc gccccagcac cagccgcagc ctctacgcct
     1141 cgtccccggg cggcgtgtat gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc
     1201 ccggggtgcg gctcctgcag gactcggtgg acttctcgct ggccgacgcc atcaacaccg
     1261 agttcaagaa cacccgcacc aacgagaagg tggagctgca ggagctgaat gaccgcttcg
     1321 ccaactacat cgacaaggtg cgcttcctgg agcagcagaa taagatcctg ctggccgagc
     1381 tcgagcagct caagggccaa ggcaagtcgc gcctggggga cctctacgag gaggagatgc
     1441 gggagctgcg ccggcaggtg gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc
     1501 gcgacaacct ggccgaggac atcatgcgcc tccgggagaa attgcaggag gagatgcttc
     1561 agagagagga agccgaaaac accctgcaat ctttcagaca ggatgttgac aatgcgtctc
     1621 tggcacgtct tgaccttgaa cgcaaagtgg aatctttgca agaagagatt gcctttttga
     1681 agaaactcca cgaagaggaa atccaggagc tgcaggctca gattcaggaa cagcatgtcc
     1741 aaatcgatgt ggatgtttcc aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc
     1801 aatatgaaag tgtggctgcc aagaacctgc aggaggcaga agaatggtac aaatccaagt
     1861 ttgctgacct ctctgaggct gccaaccgga acaatgacgc cctgcgccag gcaaagcagg
     1921 agtccactga gtaccggaga caggtgcagt ccctcacctg tgaagtggat gcccttaaag
     1981 gaaccaatga gtccctggaa cgccagatgc gtgaaatgga agagaacttt gccgttgaag
     2041 ctgctaacta ccaagacact attggccgcc tgcaggatga gattcagaat atgaaggagg
     2101 aaatggctcg tcaccttcgt gaataccaag acctgctcaa tgttaagatg gcccttgaca
     2161 ttgagattgc cacctacagg aagctgctgg aaggcgagga gagcaggatt tctctgcctc
     2221 ttccaaactt ttcctccctg aacctgaggg aaactaatct ggattcactc cctctggttg
     2281 atacccactc aaaaaggaca cttctgatta agacggttga aactagagat ggacaggtta
     2341 tcaacgaaac ttctcagcat cacgatgacc ttgaataagg atccaccgga tctagataac
     2401 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2461 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2521 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2581 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     2641 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2701 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2761 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2821 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2881 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2941 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3001 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3061 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     3121 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3181 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     3241 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3301 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     3601 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3661 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3721 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3781 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3841 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3901 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3961 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4021 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4081 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4141 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4201 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4261 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4321 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4381 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     4441 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     4501 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     4561 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     4621 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     4681 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     4741 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     4801 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     4861 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5701 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
     5761 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5821 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5881 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5941 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     6001 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     6061 gtattagtca tcgctattac catg
//
