LOCUS       sequence-213195-        9474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g00985 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g00985-bio-His
FEATURES             Location/Qualifiers
     source          1..9474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4638..4655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4707..4762
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4761..4780)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6998..7016)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7084..7188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7189..7257,7258..8049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7407..7426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8220..8808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8709..8728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8962..8979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9096..9117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9132..9137,9138..9155,9156..9162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9170..9186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9175..9197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9194..9210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9194..9210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agacactggt gtccaagatc accaccccca acaacctgcc cggcatcgat acctgcagca
     1261 actgggagga cgtgtccgtg tgcaccacca agaacacccg gaactgcatc agcggcgagg
     1321 gcaagcccaa ggactgcttc gccatcggca agagcctgtt caagaccttc cccaactgct
     1381 acgagggcgt gatcatcgac caggtcacat tcagcggctt cgaaaccctg gaaatccact
     1441 actgcgacat cgacaagatc ctgcacaagg ccaacgaggt ggtcaagagc atccacgagc
     1501 tgaaagaaaa gaccaaccag ctgaccgaga agatcaagga catccccgac ctgatcgaca
     1561 aagtgatcaa agtgaacagc gagatcagca agatcttcca ccaggacaag attaagcaca
     1621 tggaaaaaga agccaacgac ttcaagaacg ccctcaagac cacccgcaat tacatcatca
     1681 gctacgacag cctggacaag accaagcaga gcaatctgct gacctccctg ggcaagctga
     1741 tgaacaagat caaaacaaag ctgagcgaga tggataagac cctgcactcc accctggaca
     1801 ccaacaacgc catcatcgat ctggtcaaca acaatgccgc ccacgtgaag caccccaatg
     1861 atttcaacaa agccatggaa ctgtacaacg aggccatcac caaggccgat gccatcaaga
     1921 agaacatcga gaaactgaaa gagcaccgga agatctctac ccacaagaca atcttcagca
     1981 acaacatcga taagctgatc gataacctgg ccgactactt cgagaacatc aaccgggcca
     2041 tcgacgccgt gcgggacaag ctgtccaagt accagctgga aaccggcaag atggtgctgc
     2101 tgtttaagaa cgtgaacgag atccagaagc acatcaaaaa caccgacatg cacatccgga
     2161 cctgcaacta cgacttctcc gatatcgagc agaagtacag cctgatcacc gccaagatta
     2221 ccgtggaaga tggccagagc atcaccacaa gcaacaaggc aaccgtggat atccccgaag
     2281 agaaggtgga cagagtgaat gtgaacgtgg acaaagccga gaactccgac aatgaggcca
     2341 gccaggaaaa cacagccgtc aagcccaccg accacaaaga gatcgaggac agcgcctccg
     2401 aggaaaacgc cattggcgag aacggcgact acgatagcga cgaggatatc gacaccaatg
     2461 acgtgaaaga ggaccatgag aacgctatcg acagcgagta caccgtgtcc agcaccggcg
     2521 acgtgctcga ggatgaggtg gtggaagaga atgccatcgg agagaatggc gattatgact
     2581 ccgatgagga cattgacacg aacgatgtga aggaagatca cgaaaacgca atagactccg
     2641 agtatacagt gtcctctacc ggggatgtgt tggaggacga agtggttgag gaaaatgcta
     2701 tcggggaaaa cggggattac gattctgacg aggacataga tacaaacgac gtcaaagagg
     2761 atcacgaaaa cgccattgat tctgagtaca ccgtcagctc cacaggcgac gtcctggaag
     2821 acgaggtcgt cgaagagaac gcaattggcg aaaatgggga ctatgacagt gatgaagata
     2881 ttgatacgaa tgatgtcaaa gaagatcatg agaatgcaat tgatagcgag tatactgtca
     2941 gcagcacagg ggatgttctt gaagacgaag ttgtggaaga aaatgcaata ggcgagaacg
     3001 gggattatga ttcagatgaa gatatcgaca ctaacgatgt caaagaagat cacgagaacg
     3061 ctatcgattc cgagtacctg gtgtcctcca ctggggacgt tttggaagat gaggttgtcg
     3121 aggaaaacgc tataggggag aatggggact acgactccga cgaagatata gataccaacg
     3181 atgttaagga ggaccacgag gacgccattg actctgaata cctggtgtcc ctgacagggg
     3241 acgttctcga ggacgaagtc gtcgaagaaa acgccatcgg agaaaacggc gaccatgata
     3301 gtgatgagga catcgatatc gaggaagtga acgaagagga ccatgaagat gccatcgact
     3361 ccgacaacag catcagcaac agcgagaatg tggacaccac ccctaccgaa aatgtggata
     3421 caatccccac caaaaacgct aacaccgccc ccactaagaa cgcaaacgcc gctcctacaa
     3481 agaatgtcga taccattcca acaaaaaacg tcgacaccat ccccacaaag aatgccaata
     3541 ccgcacctac caaaaacgtg gtcaccactc ctaccaagaa cgccaatact gctccaacta
     3601 agaacgtcgt gaccacacca actaagaatg ccaacacagc cccaacaaag aatgtggtca
     3661 ctacacccac caagaatgca aacactgctc ccaccaaaaa tgtcgtgaca acctccaccg
     3721 gcaatgtgcg gaccaagcac accagcacca acagccacgt gctggccccc gacaccgacg
     3781 agtaccctgc ccagatcagc cagcacaaga ctattgacaa gtactaccag tccctggaac
     3841 tcgaggatga agagaacgcc gacatcagca gcagcgacaa gcccgtgtcc cccatcaatc
     3901 tggaggataa gaattccgcc tacgaccaca tgcataagac cgacaacgtg aagtccgccg
     3961 gaatcgccag ctatgatgtg aacctgggcg cgccgtcgac ctccatcacg gcctataaga
     4021 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     4081 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     4141 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     4201 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     4261 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     4321 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     4381 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     4441 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     4501 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     4561 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     4621 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     4681 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     4741 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     4801 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     4861 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     4921 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     4981 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     5041 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     5101 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     5161 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     5221 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     5281 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     5341 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     5401 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     5461 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     5521 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     5581 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     5641 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     5701 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     5761 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     5821 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     5881 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     5941 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     6001 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     6061 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     6121 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     6181 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     6241 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     6301 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     6361 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     6421 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     6481 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     6541 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     6601 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     6661 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     6721 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     6781 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     6841 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6901 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     6961 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     7021 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7081 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7141 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7321 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     7441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7501 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7561 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     7741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7801 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7861 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7921 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8041 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8101 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8341 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8881 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8941 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9001 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9061 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     9121 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9181 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     9241 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     9301 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     9361 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     9421 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
