LOCUS       sequence-213199-        7695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g02975 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g02975-bio-His
FEATURES             Location/Qualifiers
     source          1..7695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1493..1502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2859..2876
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2928..2983
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2982..3001)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5219..5237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5305..5409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5410..5478,5479..6270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5628..5647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6441..7029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6930..6949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7183..7200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7317..7338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7353..7358,7359..7376,7377..7383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7391..7407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7396..7418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7415..7431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7415..7431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 tcagagtggg cgtgatgcag cacaacaaga aacccaaggt ggtcaacgcc gtgcacaacg
     1261 tgtacgccag cctgatcgac accaacagcg ccaactccgt gacccccacc gccagcaccc
     1321 agaagaacac cgccgagaac accaataccg ccacccccga ccccaacgtg aacagcgccg
     1381 acaccaaaga gaaggacaac aacgccgccg agatcgacga caagagcatc aaccccaagt
     1441 gcatcggcga gatcatcaag cccttcagca tcgagagcaa gaccttcacc atccatggcg
     1501 gcggagccgg cgtggaaaac agcggcaagt ggatcagcaa tgccgccgac atcgatacca
     1561 acctggaaac cttcctgacc atcaagttcg agatgaacag ccagatctac agctacatca
     1621 acgacctggg ccagaacatg gccgccacca tcaacctgaa gatgagcccc gacctgaagg
     1681 acttcggcta cagctgcccc aagaacaccg tgtccgtggt ggacggcgag ttcagcaacg
     1741 tggaaaccct gctgaccgag aagctgaagt tcttcagcgg cgtgttcaac aaagaaacaa
     1801 accgggtgga catcgacatc accaagctga tccgggacaa gttcctgggc gtgggcgaca
     1861 gcggcgagaa cgtgctgaac ctggccgtga agtccggcaa caagtgcatc taccggatcc
     1921 acttcaaaga gaaccccccc accatcaaga tcatccccaa gaataccgcc tacatccaga
     1981 ccgacaagtg gaccgcctgc agcaaagagt gcaagaaaga gggcgcctac cagtgcgccc
     2041 ccatcaagtg tatcgagggc aacgagaagt gcgacaccac caagatgttc agcaagcgcg
     2101 agtgcgtgga cgtggaagat tgtgtgaacg tggaagaaca catcaacacc aagaacagca
     2161 tcggcggctg ggacctgaat ctggccaaca tcttcctgaa gtccccaggc gcgccgtcga
     2221 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     2281 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     2341 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     2401 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     2461 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     2521 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     2581 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     2641 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     2701 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     2761 ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg tggaaccaca
     2821 gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac caccattgag
     2881 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     2941 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     3001 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     3061 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     3121 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     3181 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     3241 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     3301 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     3361 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     3421 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     3481 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     3541 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     3601 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     3661 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     3721 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     3781 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     3841 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     3901 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     3961 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     4021 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     4081 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     4141 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     4201 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     4261 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     4321 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     4381 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     4441 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     4501 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     4561 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     4621 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4681 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4741 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4801 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     4861 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     4921 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4981 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5041 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     5101 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     5161 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     5221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5641 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5941 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7261 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7321 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7381 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7441 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     7501 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     7561 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     7621 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     7681 gtccaatatg accgc
//
