LOCUS       sequence-213354-        2938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP 
            and dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, 
            Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
  TITLE     FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's 
            Disease Modeling.
  JOURNAL   Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 
            10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
  PUBMED    28988985
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.08.026"; journalName: "Stem Cell Reports"; 
            date: "2017-11-14- 14"; volume: "9"; issue: "5"; pages: "1423-1431"
COMMENT     Sequence Label: pDONOR-PARK6e5-I368N
FEATURES             Location/Qualifiers
     source          1..2938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             56..757
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(836..855)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    901..956
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(955..974)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   2123..2157
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2503..2806
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaaggccg ccaccatgag
       61 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
      121 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
      181 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
      241 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
      301 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      361 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
      421 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      481 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
      541 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
      601 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
      661 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
      721 cgacctccct agcaaactgg ggcacaagct taattaacac tcctcaggtg caggctgcct
      781 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
      841 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
      901 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
      961 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     1021 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     1081 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     1141 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     1201 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     1261 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggatcc cctaggttat
     1321 acttgtcacc tagtcctttt ggtccatttg actgttaacc ttttctgtgg ctggacttat
     1381 ctgtttttaa cataaaaacc gttctccttc ctcaccctct gtatccccta actttgctat
     1441 agtgggtatt ttattttaag gaaataatta tctgcaccat tactttgaat atagggagcc
     1501 ccaactctta cttcctaatt tgaggatggt gagtgggagg gaacagaaag gatgctgggg
     1561 aaaaatggga atcaaagtgc tcctggaagg ggaagaggaa cggcctaacc ctaacagtga
     1621 ttaaggttat taggaggccg ggaatggtgg ctgacgcctg taatcccagc actttggaag
     1681 gcggaggtgg gtagatcact tgaggtcagg agtttgagac cagcctggcc aacatgatga
     1741 aaccctgtat ctactaaaca tacaaaaatt agcctggtgt ggtggcgggc acctataatc
     1801 ccagctactc gggaggctga ggtaggagaa ttgcttgaac ctggaaggtg gaggttgcag
     1861 tgagccaaga tcgtgctact gcactccagc ttggcgacag agtgagactc catctcaaaa
     1921 aaaaaaaaaa aaaaacgtat tgggagtcgt cgatgtgtgg tagccagagg ccctctcccc
     1981 tctccaccag ctatccctgt accctgcgcc agtacctttg tgtgaacaca cccagccccc
     2041 gcctcgccgc catgatgctg ctgcagctgc tggaaggcgt ggatcatctg gttcaacagg
     2101 gcatcgcgca cagagacctt aaccctagaa agatagtctg cgtaaaattg acgcatgcat
     2161 tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca
     2221 tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga
     2281 ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt acgtgacttt
     2341 taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac gtgataactt
     2401 attatatata tattttcttg ttatagatat caactagaat gctagttatt aatagtaatc
     2461 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     2521 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2581 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2641 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2701 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2761 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     2821 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     2881 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggg cgcgcgcc
//
