LOCUS       sequence-21337-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSuper mouse DGCR8-3
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..271
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     192..211
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        358..858
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(393..416)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     791..810
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(879..898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt gaagatcccc cgggctgcag gaattcgaac
       61 gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc
      121 cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca
      181 atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt
      241 tgggaatctt ataagttctg tatgagacca cagatccccc cagtgatgca gaggtaattt
      301 caagagaatt acctctgcat cactggtttt tggaactcga ggttaacgaa ttctaccggg
      361 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
      421 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
      481 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
      541 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
      601 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
      661 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
      721 ggtccggggg cgggctcagg ggcgggctca ggggcggggc ggggcgcccg aaggtcctcc
      781 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
      841 tcatctccgg gcctttcgac ctgcagccaa agcttaccat gaccgagtac aagcccacgg
      901 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccg
//
