LOCUS       sequence-213514-       11030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL3-deltaTM
FEATURES             Location/Qualifiers
     source          1..11030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1304..1325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1565..1584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1912..1933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3289..3313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3344..3932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3397..3417)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3815..3826)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3983..4482
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4018..4041)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4415..4434
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4496..5299
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4559..4578)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5169..5188
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5443..5676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5748..5869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5785..5804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5839..5858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5909..6044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6065..6083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6093..6110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6093..6109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6102..6124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6251..6706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6338..6357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6548..6569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6732..6836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6837..6905,6906..7697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7055..7074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7868..8456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8357..8376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8610..8627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8744..8765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8780..8785,8786..8803,8804..8810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8818..8834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8823..8845
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8842..8858
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8842..8858
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8877..8897
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8879..8897
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8925..9151
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9152..9332
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9379..9504
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9997..10230
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10415..10459
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10726..10842
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10876..10900
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccat
     1261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1741 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1921 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg
     1981 atctggaggt tctggtggaa tggagagcag caagcctggt ccagtgcagg ttgttttggt
     2041 tcagaaagat caacattcct ttgagctaga tgagaaagcc ttggccagca tcctcttgca
     2101 ggaccacatc cgagatcttg atgtggtggt ggtttcagtg gctggtgcct tccgaaaggg
     2161 caagtccttc attctggatt ttatgctacg atacttatat tctcagaagg aaagtggcca
     2221 ttcaaattgg ttgggtgacc cagaagaacc gttaacagga ttttcctgga gagggggatc
     2281 tgatccagaa accactggga ttcaaatctg gagtgaagtt ttcactgtgg agaagccagg
     2341 tgggaagaag gttgcagttg ttctgatgga tacccagggg gcatttgaca gccagtcaac
     2401 tgtgaaagac tgtgctacca tctttgctct aagcactatg actagttctg ttcagattta
     2461 taatttatct cagaacattc aagaagatga tcttcaacag ctgcagctct tcacagaata
     2521 cggtcgtctg gcaatggatg aaattttcca aaagcctttc cagacactga tgtttttggt
     2581 tagagattgg agtttccctt atgaatatag ctatggactc caaggaggaa tggcattttt
     2641 ggataagcgt ttacaggtga aggaacatca acatgaagaa attcagaatg ttcgaaatca
     2701 cattcactca tgtttctccg atgtcacctg ctttctctta ccacatccag gactccaggt
     2761 ggccacaagc cctgactttg atgggaaatt aaaagatatt gctggtgaat tcaaagagca
     2821 gttacaggca ctgataccgt atgtattaaa cccatctaag ttaatggaaa aggagatcaa
     2881 tggctcaaag gtcacctgtc ggggactact ggagtatttt aaggcatata ttaaaattta
     2941 tcaaggagaa gatctgcctc accccaagtc catgcttcag gccactgctg aagccaacaa
     3001 cttagcagct gcagcctctg ccaaggacat ttattataac aacatggaag aggtttgtgg
     3061 gggagagaaa ccttatttgt ctccagacat tctagaggag aagcactgtg aattcaaaca
     3121 acttgctctg gaccatttta agaagaccaa gaagatgggt gggaaggatt tcagctttcg
     3181 ttaccagcag gagctggagg aggaaatcaa ggaattatat gagaacttct gcaagcacaa
     3241 tggtagcaag aacgtcttca gcaccttccg aacccctgca tagtctagac ccagctttct
     3301 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac ctctggatta
     3361 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3421 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3481 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3541 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3601 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3661 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3721 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     3781 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3841 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3901 gagtcggatc tccctttggg ccgcctcccc gcctggacct gcaggattct gcagatatcc
     3961 ggactagtga tctaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     4021 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     4081 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4141 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4201 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     4261 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     4321 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     4381 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     4441 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag ccaatatggg
     4501 atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4561 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4621 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4681 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4741 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4801 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4861 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4921 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4981 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5041 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5101 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5161 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5221 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5281 tcttgacgag ttcttctgaa cgcgtcgccc gccccacgac ccgcagcgcc cgaccgaaag
     5341 gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg acttacaagg
     5401 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact
     5461 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5521 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5581 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5641 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5701 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5881 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6061 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6121 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6181 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6241 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6301 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6361 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6421 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6481 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6541 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6601 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6661 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6721 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6781 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6841 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6901 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6961 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7021 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7081 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7141 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7201 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7261 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7321 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7381 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7441 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7501 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7561 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7621 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7681 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7741 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7981 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8521 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8581 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8641 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8701 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8761 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8821 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     8881 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     8941 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     9001 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     9061 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     9121 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     9181 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9241 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9301 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9361 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9421 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9481 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9541 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9601 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9661 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9721 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9781 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     9841 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     9901 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     9961 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10021 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10081 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    10141 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    10201 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    10261 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    10321 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    10381 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    10441 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    10501 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    10561 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    10621 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    10681 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
    10741 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    10801 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca gtgtggtgga
    10861 attctgcaga tatcaacaag tttgtacaaa aaagcaggct ccaccatggg aaccgacatt
    10921 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
    10981 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
