LOCUS       sequence-213585-        9376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian retroviral expression of glutaminase 2 isoform GAB (wild 
            type) with V5 and 6xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Dias MM, Adamoski D, Dos Reis LM, Ascencao CFR, de Oliveira KRS, 
            Mafra ACP, da Silva Bastos AC, Quintero M, de G Cassago C, Ferreira 
            IM, Fidelis CHV, Rocco SA, Bajgelman MC, Stine Z, Berindan-Neagoe I,
            Calin GA, Ambrosio ALB, Dias SMG
  TITLE     GLS2 is protumorigenic in breast cancers.
  JOURNAL   Oncogene. 2020 Jan;39(3):690-702. doi: 10.1038/s41388-019-1007-z. 
            Epub 2019 Sep 20.
  PUBMED    31541193
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-1007-z"; journalName: "Oncogene"; date: 
            "2020-01"; volume: "39"; issue: "3"; pages: "690-702"
COMMENT     Sequence Label: pQC hGAB.wt V5-His IRES G418
FEATURES             Location/Qualifiers
     source          1..9376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4167..4208
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4218..4235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4395..4857
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4451..4468)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4678..4697
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4871..5665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4925..4944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5535..5554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6082..6507
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6610..6632)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6768..6788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6773..7102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6953..7088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7124..7143
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7224..7241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7395..7983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7475..7494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8154..8945,8946..9014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8777..8796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9015..9119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9187..9205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9305..9376,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tggaagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggggatcc actagtccag tgtggtggaa
     2281 ttgcccttac catgggccgc tccatgaagg ctctgcagaa ggccctgagc cgggctggca
     2341 gtcactgcgg gcgaggaggc tggggtcacc cgagccggag ccccctcctt ggcgggggcg
     2401 tccggcacca cctcagtgag gccgcggcgc agggcagaga gacgccacac agccaccagc
     2461 cgcagcacca ggatcatgat tcatcagaaa gtggcatgct gtcccgcctg ggtgatttgc
     2521 tcttttacac tattgctgaa ggacaggaac gaatccctat ccacaagttc accactgcac
     2581 taaaggccac tggactgcag acatcagatc ctcggctccg agactgcatg agcgagatgc
     2641 accgcgtggt ccaagagtcc agtagtggtg gcctcttgga ccgagatctc ttccgaaagt
     2701 gtgtgagcag caacattgtg ctcctgaccc aggcattccg aaagaagttt gtcattcctg
     2761 attttgagga gttcacgggc catgtggatc gcatctttga ggatgtcaaa gagctcactg
     2821 gaggcaaagt ggcagcctac atccctcagc tggccaagtc aaacccagac ctgtggggtg
     2881 tctccctgtg cactgtggat ggtcaacggc actctgtggg ccacacaaag atccccttct
     2941 gcctgcagtc ctgtgtgaag cccctcacct atgccatctc cataagcacc ctaggcactg
     3001 actacgtgca caagtttgtg ggcaaagagc caagtggcct gcgctacaac aagctctccc
     3061 tcaatgagga aggaatcccc cataacccca tggtcaatgc tggtgccatt gttgtcagct
     3121 ccctgatcaa gatggactgt aacaaagcag agaagtttga ttttgtgttg cagtatctca
     3181 acaaaatggc tgggaatgaa tacatgggtt tcagcaatgc cacattccag tcagagaagg
     3241 aaacagggga tcggaattat gccatcggct attatctcaa ggaaaagaag tgctttccta
     3301 agggggtgga catgatggct gcccttgatc tctacttcca gctgtgttct gtggaggtca
     3361 cttgtgaatc aggcagtgtc atggcagcca ccctcgccaa cggtgggatc tgccccatca
     3421 caggcgagag tgtgctgagt gctgaagcag tgcgcaacac cctcagcctc atgcattcct
     3481 gcggcatgta tgacttctct ggccagtttg ccttccacgt gggcctgcca gccaagtcag
     3541 ctgtatcagg agccatcctc ctggtggtac ccaatgtcat gggaatgatg tgcctgtcac
     3601 ccccattgga caagctgggg aacagccata gggggaccag cttctgccag aagttggtgt
     3661 ctctcttcaa tttccacaac tatgacaacc tgaggcactg tgctcggaag ttagacccac
     3721 ggcgtgaagg ggcagaaatt cggaacaaga ctgtggtcaa cctgttattt gctgcctata
     3781 gtggcgatgt ctcagctctt cgaaggtttg ccttgtcagc catggatatg gaacagaaag
     3841 actatgactc gcgcacagct ctgcatgttg ctgcagctga aggacacatc gaagttgtta
     3901 aattcctgat cgaggcttgc aaagtgaatc cttttgccaa ggacaggtgg ggcaacattc
     3961 ccctggatga tgctgtgcag ttcaaccatc tggaggtggt caaactgctt caagattacc
     4021 aggactccta cacactctct gaaactcagg ctgaggcagc agctgaggcc ctgtccaaag
     4081 agaacttaga aagcatggta aagggcaatt ctgcagatat ccagcacagt ggcggccgct
     4141 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     4201 attctacgcg taccggtcat catcaccatc accattgagt ttaaactcca aagcatcgat
     4261 ggtagcggcc gcggtgaatt ccgccccccc cccycccccc cccctaacgt tactggccga
     4321 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     4381 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4441 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4501 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4561 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     4621 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     4681 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4741 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     4801 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     4861 tgccacaacc atggctgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4921 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4981 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5041 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5101 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5161 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5221 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5281 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5341 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5401 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5461 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5521 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5581 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5641 tcgccttctt gacgagttct tctgagcggg atcggctaga gtcgacccgg acgggacgct
     5701 cctgcgcctg atacagaacg aattgcttgc aggcatctca tgagtgtgtc ttcccgtttt
     5761 ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc tgcgcgacgg cgagctgctc
     5821 accacccact cgagggctgc agcgctgcag aggccgagtg cagaactgct ccaaagggac
     5881 ctcaaggctt tccgagggac actaggctga ctccatcgag ccagtgtaga gataagctta
     5941 tcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca
     6001 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt
     6061 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     6121 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
     6181 aaggtcagga acagatggaa cagggtcgac cctagagaac catcagatgt ttccagggtg
     6241 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     6301 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     6361 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     6421 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     6481 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc
     6541 cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga
     6601 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6661 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     6721 agccatgacc cagtcacgta gcgatagcgg agtgtagatc cggctgtgga atgtgtgtca
     6781 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6841 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6901 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7081 tggaggccta ggcttttgca aaaagcttac tggcttaact atgcggcatc agagcagatt
     7141 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     7201 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7801 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8461 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8761 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9121 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9181 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattagcttg gccattgcat
     9241 acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac attaccgcca
     9301 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     9361 agcccatata tggagt
//
