LOCUS       sequence-213659-        9956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of GFP and Luciferase under the control of E2F1 
            responsive element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9956)
  TITLE     Di Giovanni Lab neuroscience plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGreenFire1_E2F1RE
FEATURES             Location/Qualifiers
     source          1..9956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    449..682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             867..911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1178..1294
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1394..1418
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1506..2171
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             1506..2171
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             2313..2366
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2373..4025
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2439..2457)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    4036..4624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4089..4109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4507..4518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4682..4893
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4906..5174
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5202..5996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5256..5275)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5866..5885
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6068..6301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6373..6494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6410..6429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6464..6483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6534..6669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6702..6718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6702..6718)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6715..6737)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6726..6742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6750..6756,6757..6774,6775..6780))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6795..6816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6933..6950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7104..7692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7184..7203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7863..8654,8655..8723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8486..8505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8724..8828)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8896..8914
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     9074..9093
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9287..9309
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9301..9318
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9302..9318
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9333..9559
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9560..9740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9787..9912
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
       61 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
      121 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
      181 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
      241 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
      301 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
      361 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
      421 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
      481 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
      541 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
      601 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
      661 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
      721 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
      781 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
      841 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
      901 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
      961 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     1021 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     1081 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     1141 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     1201 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     1261 aattacaaaa acaaattaca aaattcaaaa ttttatcgat gaattctttc gcgccaaatt
     1321 tcgcgccaaa tttcgcgcca aatttcgcgc caaaactagt taggcgtgta cggtgggagg
     1381 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     1441 gttttgacct ccatagaaga caccgggacc gatccagcct ctcgacattc gttggatccg
     1501 ccaccatgcc cgccatgaag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     1561 tcgagctggt gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc aacaagatga
     1621 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     1681 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     1741 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     1801 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca
     1861 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     1921 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca
     1981 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg
     2041 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     2101 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca
     2161 tcgccttcgc cagatctcga gatatcagcc atggcttccc gccggcggtg gcggcgcagg
     2221 atgatggcac gctgcccatg tcttgtgccc aggagagcgg gatggaccgt caccctgcag
     2281 cctgtgcttc tgctaggatc aatgtgaccg gtgagggcag aggaagtctt ctaacatgcg
     2341 gtgacgtgga ggagaatccc ggcccttccg gtatggaaga cgccaaaaac ataaagaaag
     2401 gcccggcgcc attctatcca ctagaggatg gaaccgctgg agagcaactg cataaggcta
     2461 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga
     2521 acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat
     2581 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta
     2641 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata
     2701 atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca
     2761 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta
     2821 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat
     2881 ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca
     2941 aaacaattgc actgataatg aactcctctg gatctactgg gttacctaag ggtgtggccc
     3001 ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc
     3061 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt
     3121 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag
     3181 aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa
     3241 ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac
     3301 acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaggcg gttgcaaaac
     3361 gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc
     3421 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg
     3481 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat
     3541 tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg
     3601 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg
     3661 aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga taccaggtgg
     3721 cccccgctga attggagtcg atattgttac aacaccccaa catcttcgac gcgggcgtgg
     3781 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg
     3841 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga
     3901 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac
     3961 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat
     4021 tgtaatcgac tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4081 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4141 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4201 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4261 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4321 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4381 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4441 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4501 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4561 cctcttccgc atcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4621 ccgcctggaa ttaattcgag ctcggtacca aggatctgcg atcgctccgg tgcccgtcag
     4681 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4741 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4801 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4861 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     4921 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     4981 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5041 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5101 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     5161 cgccgttaca gatccaagct gtgaccggcg cctacgtcga gatgattgaa caagatggat
     5221 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5281 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5341 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5401 tatcgtggct ggccgcgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5461 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5521 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5581 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5641 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5701 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5761 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5821 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5881 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5941 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaggta
     6001 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6061 gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact
     6121 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6181 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6241 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6301 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6361 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6481 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6541 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6601 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6661 ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg
     6721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8821 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     8881 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     8941 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     9001 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     9061 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     9121 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     9181 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     9241 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     9301 tgtaaaacga cggccagtgc caagctgacg cgtgtagtct tatgcaatac tcttgtagtc
     9361 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     9421 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     9481 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     9541 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     9601 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9661 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9721 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9781 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9841 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9901 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcg
//
