LOCUS       sequence-21390-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Zhang Y, Musci T, Derynck R
  TITLE     The tumor suppressor Smad4/DPC 4 as a central mediator of Smad 
            function.
  JOURNAL   Curr Biol. 1997 Apr 1. 7(4):270-6.
  PUBMED    9094310
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            1997 Apr 1. 7(4):270-6."
COMMENT     Sequence Label: pRK Smad3N Flag
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      539..548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    552..686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(589..608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     643..662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggtgtccnct cccaggtcca actgcacctc ggttctatcg attgaattcc ccgggatcca
       61 tgtcgtccat cctgcctttc actcccccga tcgtgaagcg cctgctgggc tggaagaagg
      121 gcgagcagaa cgggcaggag gagaaatggt gcgagaaggc ggtcaagagc ctggtcaaga
      181 aactcaagaa gacggggcag ctggacgagc tggagaaggc catcaccacg cagaacgtca
      241 acaccaagtg catcaccatc cccaggtccc tggatggccg gttgcaggtg tcccatcgga
      301 aggggctccc tcatgtcatc tactgccgcc tgtggcgatg gccagacctg cacagccacc
      361 acgagctgcg ggccatggag ctgtgtgagt tcgccttcaa tatgaagaag gacgaggtct
      421 gcgtgaatcc ctaccactac cagagagtag agacaccagt tctacctcct gtgttggtgc
      481 cacgccacac agaggtcgac atggactaca aggacgacga cgacaagtga taagcttggc
      541 cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      601 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
      661 tcaatgtatc ttatcatgtc tggatcggga attaattcng cgcancancn tggnctgaaa
      721 t
//
