LOCUS       sequence-213901-        6312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABA Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, 
            Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
            Ahrens MB, Kullmann DM, Looger LL
  TITLE     A genetically encoded fluorescent sensor for in vivo imaging of 
            GABA.
  JOURNAL   Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
            Epub 2019 Jul 15.
  PUBMED    31308547
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-019-0471-2"; journalName: "Nat Methods"; date: 
            "2019-08"; volume: "16"; issue: "8"; pages: "763-770"
COMMENT     Sequence Label: pAAV.hSynap.iGABASnFR.R205A
FEATURES             Location/Qualifiers
     source          1..6312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1619..1638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1857..1961)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1987..2442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2124..2145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2336..2355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2623..2752
                     /label=AAV2 ITR
     promoter        2828..3275
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3304..3313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3310..3369
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     4416..4437
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4540..4561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             5068..5097
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5101..5247
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    5263..5851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5316..5336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5734..5745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5854..5870)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5855..5871)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5900..6021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5911..5930)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5965..5984
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6183..6312
                     /label=AAV2 ITR
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2041 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2101 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2161 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2221 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2281 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2341 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2401 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2461 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg
     2521 ctcgctcgct cactgaggcc gcccgggcaa agcccggggc cagggtttyc ccagtcacgg
     2581 yggggaaggg cgacggtgcg ggcctcttcg ctattacgcc agctgcgcgc tcgctcgctc
     2641 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
     2701 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
     2761 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat ccagggccct
     2821 gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt gcctacctga
     2881 cgaccggccc cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc
     2941 tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag
     3001 caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg
     3061 aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc
     3121 gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc
     3181 acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg
     3241 gcagcggagg agtcgtgtcg tgcctgagag cgcagtccaa ttcaagctgc tagcaaggat
     3301 cccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt
     3361 tccactggtg acagatctga aagcatcaat ttcgtgagct ggggcggtag cacccaggat
     3421 gcgcagaagc aggcctgggc cgacccgttc agcaaggcca gcggcattac cgtggtccag
     3481 gatgggccca ccgactacgg caaactcaag gccatggtcg aaagcggcaa cgtgcagtgg
     3541 gacgtggtgg atgtagaggc cgacttcgcc ttgcgcgccg ccgccgaagg cctgctcgaa
     3601 cccctggatt tctcggtgat ccagcgcgac aagatcgacc cgcgcttcgt ctccgaccat
     3661 ggcgtgggct cgttcctgtt ctctttcgtg ctcggctaca acgaaggcaa gctcggagcc
     3721 agcaagcccc aggactggac cgcgctgttc gacaccaaga cctaccccgg caaacgcgcc
     3781 ctctacaaat ggccgagccc tggcgtgctc gaactggccc tgctcgccga cggcgtaccg
     3841 gccgacaagc tctacccgct ggacctggac cgcgccttca agaaactcga caccatcaag
     3901 aaagacatcg tctggtgggg cggcggtgca cagtcgcagc agctgctggc ctccggcgaa
     3961 gtcagcatgg gccagttctg gaacggtgcc atccacgccc tgcaagaaga cggcgcgcca
     4021 gtgggcgtga gctggaagca gaacctggtg atggccgaca tcctcgtcgt gcctaaaggc
     4081 acgaagaaca aagccgccgc gatgaagttc ctggccagtg ccagcagcgc caaaggccag
     4141 gacgactttt ccgccctgac cgcctatgct ccagtgaaca tcgacagtgt gcagcgcctc
     4201 gaccttgcgc aggtccggat caccgccgac aagcagaaga acggcatcat ggcgaacttc
     4261 aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4321 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4381 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     4441 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     4501 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4561 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     4621 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4681 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4741 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag
     4801 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4861 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4921 gagtacaact ggaacgccaa cctggcaccg aacctgccga ccgcctacgt caaggatcag
     4981 atcaccctcg atttcgccta ctgggccaag aacggtccgg ccattgcgac acggtggaat
     5041 gaatggctag tcaaactgca ggtcgacgaa caaaaactca tctcagaaga ggatctgaat
     5101 gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc ctttaaggtg
     5161 gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct tatcatcctc
     5221 atcatgcttt ggcagaagaa gccacgttag aagcttatcg ataatcaacc tctggattac
     5281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5521 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5641 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5821 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg acccgggcgg
     5881 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     5941 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6001 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6061 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     6121 aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     6181 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     6241 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     6301 gcgagcgcgc ag
//
