LOCUS       sequence-213907-        2365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human beta 1 ectodomain with secretion peptide, purification
            tags, and C-terminal clasp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Li J, Su Y, Xia W, Qin Y, Humphries MJ, Vestweber D, Cabanas C, Lu 
            C, Springer TA
  TITLE     Conformational equilibria and intrinsic affinities define integrin 
            activation.
  JOURNAL   EMBO J. 2017 Mar 1;36(5):629-645. doi: 10.15252/embj.201695803. Epub
            2017 Jan 25.
  PUBMED    28122868
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201695803"; journalName: "EMBO J"; date: "2017-03-1- 
            1"; volume: "36"; issue: "5"; pages: "629-645"
COMMENT     Sequence Label: human b1 ecto-pIRES
FEATURES             Location/Qualifiers
     source          1..2365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2209..2229
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2332..2355
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atggatatga gagttcctgc tcaattgctg gggttgctct tgctctggtt cagtggggtg
       61 ttggggcaga cggacgagaa tcgatgtttg aaagcgaatg caaaaagctg cggagagtgc
      121 attcaggccg gaccgaattg tggatggtgt acaaattcga catttttgca agagggaatg
      181 cccacgtcgg cccgatgtga tgacctcgag gccttgaaaa agaaaggatg tccaccggat
      241 gacatcgaga acccacgggg ctcaaaggac atcaaaaaga acaagaatgt cacaaaccgc
      301 tcgaagggaa ctgctgaaaa gcttaaaccg gaggacatca cgcagatcca gccccaacag
      361 ctggtgctcc gattgcggtc cggtgagcca cagacgttta ctctcaagtt caagagagcg
      421 gaggattacc cgatcgatct ttactatctt atggaccttt cgtactccat gaaggatgat
      481 ttggaaaatg tcaagagcct cggtactgac ttgatgaatg aaatgcgccg cattaccagc
      541 gatttccgaa tcggattcgg atcatttgtg gaaaagaccg tcatgcccta catttcgacc
      601 actccggcaa agttgcgcaa cccgtgcacc tcagaacaga actgcacctc gcctttttcg
      661 tataagaacg tcctcagctt gacaaacaag ggtgaagtgt tcaatgagct ggtcggaaag
      721 caaagaatct cgggaaacct cgatagcccg gagggagggt ttgatgcaat catgcaagtg
      781 gcggtatgcg ggtcccttat tggctggaga aacgtgacca gactcctggt gttttccact
      841 gatgcgggat ttcatttcgc cggagacggg aaactgggag ggattgtact tccgaatgac
      901 ggacagtgtc atttggagaa caacatgtat acgatgtccc actactatga ctacccctcg
      961 atcgcgcacc tcgtccaaaa actgagcgaa aacaatatcc agactatctt tgcggtcacg
     1021 gaggagtttc agcccgtgta taaagaactg aaaaacttga tccctaagtc cgcagtcgga
     1081 acgctgtcgg ccaacagctc gaatgtgatc cagttgatca tcgatgcgta taactcactt
     1141 tcatcagagg tcatccttga gaacggaaag cttagcgaag gagtgacaat ctcgtacaaa
     1201 tcatattgca agaatggagt gaatggcacc ggcgagaatg gccggaaatg ttcaaacatt
     1261 tccatcggcg acgaggtcca gtttgagatc agcattactt ccaataagtg ccctaagaaa
     1321 gactcggact cattcaaaat tcggcctctc gggtttaccg aagaggtaga ggtgatcctc
     1381 cagtacattt gtgaatgcga gtgtcaatca gagggtatcc ccgaaagccc caagtgtcat
     1441 gagggtaacg gaacgttcga atgcggggca tgtagatgta acgaaggtcg cgtcggaagg
     1501 cactgcgaat gctcgactga tgaggtaaac tcggaagaca tggatgcgta ttgccgaaaa
     1561 gagaattcga gcgaaatctg ctcaaacaac ggggaatgtg tgtgcggaca atgtgtctgc
     1621 cggaaacggg ataacacaaa tgagatctac tccggaaagt tttgcgaatg cgacaatttc
     1681 aactgtgaca ggtccaacgg tcttatctgt ggtggtaatg gagtctgcaa gtgccgcgtc
     1741 tgcgagtgta atccgaatta cacaggctca gcgtgtgatt gcagcctcga cacgtcaaca
     1801 tgcgaggcgt ccaacgggca gatttgtaac gggcgaggga tttgcgagtg tggggtgtgc
     1861 aaatgcactg accccaaatt ccaggggcag acgtgcgaga tgtgccagac gtgtcttggt
     1921 gtctgtgcgg agcataagga atgcgtgcag tgcagagcgt tcaacaaagg ggaaaagaaa
     1981 gatacgtgta cgcaagaatg ttcgtatttc aatatcacga aggtggagtc gagggataag
     2041 ctcccgcagc cagtacagcc ggaccccgtc agccattgca aggaaaagga cgtggatgat
     2101 tgctggttct atttcacata ctccgtgaat ggtaataatg aggtaatggt acatgtcgtg
     2161 gagaaccccg aatgccccac gggtcccgac gggcaccaac accaactcga gaatctctac
     2221 ttccaaggtg gggaaggccc agcccagtgt aagaagaaac tccaggcgct gaagaaaaag
     2281 aacgcgcagt tgaaatggaa gctccaagca ctgaaaaaga aattggcgca gcatcaccac
     2341 catcaccatc atcattaaag gatcc
//
