LOCUS       sequence-213913-        6715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABA Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, 
            Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
            Ahrens MB, Kullmann DM, Looger LL
  TITLE     A genetically encoded fluorescent sensor for in vivo imaging of 
            GABA.
  JOURNAL   Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
            Epub 2019 Jul 15.
  PUBMED    31308547
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-019-0471-2"; journalName: "Nat Methods"; date: 
            "2019-08"; volume: "16"; issue: "8"; pages: "763-770"
COMMENT     Sequence Label: pAAV.GFAP.iGABASnFR.F102G
FEATURES             Location/Qualifiers
     source          1..6715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    131..152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(441..1029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(521..540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1200..1991,1992..2060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2061..2165)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2191..2646)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2328..2349)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2540..2559
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2898..3027
                     /label=AAV2 ITR
     regulatory      3779..3788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3785..3844
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     4891..4912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5015..5036)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             5543..5572
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5576..5722
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    5738..6326
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5791..5811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6209..6220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6329..6345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6330..6346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    6375..6496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6386..6405)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6440..6459
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
       61 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagmag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatcattact acaaggaacc cctagtgatg
      241 gagttggcca ctccctctct gcgcgctcgc tcgctcactg actcgctgcg ctcggtcgtt
      301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      841 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2161 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     2221 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     2281 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     2341 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     2401 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     2461 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     2521 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     2581 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     2641 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2701 cttcgctatt acgccagctg cgcgctcgct cgctcactga ggccgccagg gcgatggcaa
     2761 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     2821 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2881 cttcgctatt acgccagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
     2941 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     3001 tggccaactc catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat
     3061 ctacgtagcc atgctctagg aagatccaac atatcctggt gtggagtagg ggacgctgct
     3121 ctgacagagg ctcgggggcc tgagctggct ctgtgagctg gggaggaggc agacagccag
     3181 gcctttgtct gcaagcagac ctggcagcat tgggctggcc gccccccagg gcctcctctt
     3241 catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg cacagtgcct
     3301 gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt gagcaggggg
     3361 cttgcattgc accccagcct gacagcctgg catcttggga taaaagcagc acagccccct
     3421 aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga acaaggctct attcagcctg
     3481 tgcccaggaa aggggatcag gggatgccca ggcatggaca gtgggtggca gggggggaga
     3541 ggagggctgt ctgcttccca gaagtccaag gacacaaatg ggtgagggga gagctctccc
     3601 catagctggg ctgcggccca accccacccc ctcaggctat gccagggggt gttgccaggg
     3661 gcacccgggc atcgccagtc tagcccactc cttcataaag ccctcgcatc ccaggagcga
     3721 gcagagccag agcaggttgg agaggagacg catcacctcc gctgctcgcg gggatcccgc
     3781 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
     3841 tggtgacaga tctgaaagca tcaatttcgt gagctggggc ggtagcaccc aggatgcgca
     3901 gaagcaggcc tgggccgacc cgttcagcaa ggccagcggc attaccgtgg tccaggatgg
     3961 gcccaccgac tacggcaaac tcaaggccat ggtcgaaagc ggcaacgtgc agtgggacgt
     4021 ggtggatgta gaggccgact tcgccttgcg cgccgccgcc gaaggcctgc tcgaacccct
     4081 ggatttctcg gtgatccagc gcgacaagat cgacccgcgc ttcgtctccg accatggcgt
     4141 gggctcgttc ctgggttctt tcgtgctcgg ctacaacgaa ggcaagctcg gagccagcaa
     4201 gccccaggac tggaccgcgc tgttcgacac caagacctac cccggcaaac gcgccctcta
     4261 caaatggccg agccctggcg tgctcgaact ggccctgctc gccgacggcg taccggccga
     4321 caagctctac ccgctggacc tggaccgcgc cttcaagaaa ctcgacacca tcaagaaaga
     4381 catcgtctgg tggggcggcg gtgcacagtc gcagcagctg ctggcctccg gcgaagtcag
     4441 catgggccag ttctggaacg gtcgcatcca cgccctgcaa gaagacggcg cgccagtggg
     4501 cgtgagctgg aagcagaacc tggtgatggc cgacatcctc gtcgtgccta aaggcacgaa
     4561 gaacaaagcc gccgcgatga agttcctggc cagtgccagc agcgccaaag gccaggacga
     4621 cttttccgcc ctgaccgcct atgctccagt gaacatcgac agtgtgcagc gcctcgacct
     4681 tgcgcaggtc cggatcaccg ccgacaagca gaagaacggc atcatggcga acttcaagat
     4741 ccgccacaac gtggaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgtgct
     4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4921 cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga gcatgagcaa
     4981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5041 cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac
     5101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5161 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     5221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcagct tcaaggacga
     5281 cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5401 caactggaac gccaacctgg caccgaacct gccgaccgcc tacgtcaagg atcagatcac
     5461 cctcgatttc gcctactggg ccaagaacgg tccggccatt gcgacacggt ggaatgaatg
     5521 gctagtcaaa ctgcaggtcg acgaacaaaa actcatctca gaagaggatc tgaatgctgt
     5581 gggccaggac acgcaggagg tcatcgtggt gccacactcc ttgcccttta aggtggtggt
     5641 gatctcagcc atcctggccc tggtggtgct caccatcatc tcccttatca tcctcatcat
     5701 gctttggcag aagaagccac gttagaagct tatcgataat caacctctgg attacaaaat
     5761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6241 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6301 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg ggcggccgct
     6361 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     6421 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     6481 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     6541 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     6601 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     6661 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actga
//
