LOCUS       sequence-213921-       10951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Galactose-inducible Ty3 with extra PPT- used for random bar code 
            library.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Patterson K, Shavarebi F, Magnan C, Chang I, Qi X, Baldi P, 
            Bilanchone V, Sandmeyer SB
  TITLE     Local features determine Ty3 targeting frequency at RNA polymerase 
            III transcription start sites.
  JOURNAL   Genome Res. 2019 Aug;29(8):1298-1309. doi: 10.1101/gr.240861.118. 
            Epub 2019 Jun 27.
  PUBMED    31249062
REFERENCE   2  (bases 1 to 10951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.240861.118"; journalName: "Genome Res"; date: "2019-08";
            volume: "29"; issue: "8"; pages: "1298-1309"
COMMENT     Sequence Label: pKN3097
FEATURES             Location/Qualifiers
     source          1..10951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    190..307
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     misc_feature    6413..7575
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      6738..7575
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(7910..8713)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8714..8934)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8959..8977)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9045..9149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9150..9218,9219..10010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9368..9387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10181..10769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10670..10689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10923..10940
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacccgc ggatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa
       61 aaaactaatc gcattatcat cctatggttg ttaatttgat tcgttcattt gaaggtttgt
      121 ggggccaggt tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc
      181 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
      241 agacgaggac gcacggagga gagtctttct tcggagggct gtcacccgct cggcggcttc
      301 taatccgtac ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga
      361 aggttttttt aggctaatcg acctcgactc tagaggatcc ccctgaacta cccaaagtat
      421 aaatgcctga acaattagtt tagatccgag attccgcgct tccaccactt agtatgattc
      481 atattttata taatatataa gataagtaac attccgtgaa ttaatctgat aaactgtttt
      541 gacaactggt tacttcccta agactgttta tattaggatt gtcaagacac tccggtatta
      601 ctcgagcccg taatacaaca cctggtagcg ttaaaggtta ctaattgttc aaacgaacca
      661 tcgaaaagcc gaacctagct acaccacacc ccagtatgag ctttatggat caaatcccag
      721 gaggaggaaa ttatccaaaa ctcccagtag aatgccttcc taacttcccg atccaaccat
      781 ctttgacctt cagaggtaga aatgactcgc ataaactgaa aaactttatc tccgaaataa
      841 tgttaaacat gtctatgata tcttggccga atgatgccag tcgtattgtg tactgcagaa
      901 gacatttatt aaaccccgct gctcagtggg ctaatgactt tgtacaagaa caaggtatac
      961 ttgaaataac attcgacaca ttcatacaag gattatatca gcatttctat aagccaccag
     1021 atatcaataa aatctttaat gcaatcacgc aactttccga agctaaactt ggtattgagc
     1081 gtctcaacca acgattcaga aagatttggg acagaatgcc accagacttc atgaccgaaa
     1141 aagctgccat aatgacatat actaggctat tgacaaagga aacctataat attgtcagaa
     1201 tgcacaaacc agagacatta aaagacgcca tggaagaggc ttaccagaca actgcactaa
     1261 ctgaaagatt cttcccagga ttcgaacttg atgctgatgg agacactatc atcggtgcca
     1321 caacccactt acaagaagaa tacgactctg actatgattc agaagataat ctgacccaga
     1381 atggatacgt ccataccgta aggacaagaa gatcttacaa taaaccaatg tcaaatcatc
     1441 gaaacaggag aaataacaac ccatctagag aagaatgtat aaaaaatcgg ctatgcttct
     1501 attgtaagaa agagggacat cgcctgaacg aatgtagagc acgtaaggcg agttctaacc
     1561 gatcttgaac tcgaatcaaa agaccaacaa actcctttta tcaaaacctt accaattgta
     1621 cactatatcg ccatccccga gatggacaat accgccgaaa aaaccataaa aatacaaaac
     1681 acgaaagtaa aaaccctgtt tgacagtgga tcacccacgt catttatccg aagagatatt
     1741 gtagaacttc tcaaatacga aatctacgag acccctccac tccgttttag aggattcgta
     1801 gccaccaaat ccgccgttac atccgaagca gtcaccattg acctcaaaat caatgacctg
     1861 catataactt tagccgcgta catactggat aacatggact accaattgtt aattggaaat
     1921 ccaatcttac gccgctaccc gaaaatcctg cacacagtac tgaataccag agagagcccc
     1981 gactccttaa agcccaagac ttatcgctcc gaaaccgtta ataacgttag aacctactcc
     2041 gctggtaatc gtggtaaccc cagaaacata aaactgtctt ttgcccccac cattctcgaa
     2101 gcaactgacc cgaaatccgc tggtaatcgt ggtgactcca gaaccaaaac cctgtctctt
     2161 gcaaccacta ctcctgcagc aattgacccg cttacgaccc ttgataaccc aggtagtact
     2221 caaagtacat ttgcgcaatt cccgatacct gaagaagcga gcatcctaga agaggatgga
     2281 aaatactcca acgttgtctc aaccattcag agtgtagaac ctaatgctac tgatcacagc
     2341 aataaggaca ccttttgcac tttgccagtt tggttacaac agaagtatag agagatcata
     2401 cgtaatgatc tcccaccaag acctgccgac attaataaca tccccgtaaa acatgatatt
     2461 gaaattaaac ctggcgcaag actacctcga ctacagccat accatgttac agaaaagaac
     2521 gaacaagaaa tcaacaaaat agttcaaaaa ctgctcgata acaagttcat tgttccctca
     2581 aagtcgcctt gcagctcccc tgtagtcctc gtcccgaaga aagacggtac cttccgactc
     2641 tgcgtcgatt accgcaccct gaacaaagct accatctccg acccattccc attacccaga
     2701 atcgacaacc tattgagccg tattggaaat gcccagatat ttaccacgct agatttgcat
     2761 agtggttacc accagatccc gatggaaccc aaagaccgct acaaaaccgc ctttgtcaca
     2821 ccatccggta agtatgaata taccgtcatg ccatttggct tagtcaatgc acctagtaca
     2881 ttcgcaagat acatggctga tacatttaga gacctgagat tcgtcaatgt ttaccttgat
     2941 gatatattaa tattctccga atctccagaa gaacattgga aacatttaga cacggtacta
     3001 gaaagattaa agaacgagaa cctcattgtt aagaagaaaa aatgtaaatt tgcatctgaa
     3061 gaaactgagt ttttaggcta tagtattgga atccagaaaa tagctccact acagcacaaa
     3121 tgtgcagcaa tccgagactt tccgacgcct aaaacagtaa aacaagcaca gagattttta
     3181 ggaatgatta attactacag acgattcatt ccaaattgct ccaagattgc acagccaatc
     3241 caactgttta tttgtgacaa aagtcaatgg acagaaaaac aagacaaggc aattgataaa
     3301 ctaaaagacg ccttgtgtaa ctcccccgtc ctagtaccat tcaacaacaa agcaaactac
     3361 cgacttacaa cagacgcctc aaaagacggc attggtgctg ttctagaaga agtcgacaac
     3421 aagaacaaac ttgttggtgt cgtcggttac ttctctaaat ccttagagag tgcccagaaa
     3481 aactatcctg ctggcgaatt agaactactt ggaattatca aagcactcca ccacttccga
     3541 tatatgcttc acggaaagca tttcacgtta agaacagacc acattagttt gttatcatta
     3601 caaaacaaga acgaacccgc acgacgcgtg caacgctggt tagatgacct agccacatat
     3661 gacttcacct tagaatacct agctggaccc aagaacgttg tcgcagatgc catatcccgt
     3721 gccgtatata ctataacccc cgaaacatcc cgacctatcg acacagaaag ctggaaatct
     3781 tactacaaat cagacccatt atgtagtgct gtcttaattc atatgaaaga attgacacaa
     3841 cacaacgtca cacctgaaga tatgtcagcc ttccgtagtt accagaagaa actcgaacta
     3901 tcagagacct tccgaaagaa ttattcccta gaagacgaaa tgatctatta ccaagaccga
     3961 ctagtagtac caataaaaca acagaacgca gttatgagac tatatcatga ccatacctta
     4021 tttggaggac attttggtgt aacagtgacc cttgcgaaaa tcagcccaat ttactattgg
     4081 ccaaaattac aacattcgat catacaatac atcaggacct gcgtacaatg tcaactaata
     4141 aaatcacacc gaccacgctt acatggacta ttacaaccac tccctatagc agaaggaaga
     4201 tggcttgata tatcaatgga ttttgtgaca ggattacccc cgacatcaaa taacttgaat
     4261 atgatcctcg tcgtagttga tcgtttttcg aaacgcgctc acttcatagc tacaaggaaa
     4321 accttagacg caacacaact aatagatcta ctctttcgat acattttttc atatcatggt
     4381 tttcccagga caataaccag tgatagagat gtccgtatga ccgccgacaa atatcaagaa
     4441 ctcacgaaaa gactaggaat aaaatcgaca atgtcttccg cgaaccaccc ccaaacagat
     4501 ggacaatccg aacgaacgat acagacatta aacaggttac taagagccta tgcttcaacc
     4561 aatattcaga attggcatgt atatttacca caaatcgaat ttgtttacaa ttctacacct
     4621 actagaacac ttggaaaatc accatttgaa attgatttag gatatttacc gaatacccct
     4681 gctattaagt cagatgacga agtcaacgca agaagtttta ctgccgtaga acttgccaaa
     4741 cacctcaaag cccttaccat ccaaacgaag gaacagctag aacacgctca aatcgaaatg
     4801 gaaactaata acaatcaaag acgtaaaccc ttattgttaa acataggaga tcacgtatta
     4861 gtgcatagag atgcatactt caagaaaggt gcttatatga aagtacaaca aatatacgtc
     4921 ggaccatttc gagttgtcaa gaaaataaac gataacgcct acgaactaga tttaaactct
     4981 cacaagaaaa agcacagagt tattaatgta caattcctga aaaagtttgt ataccgtcca
     5041 gacgcgtacc caaagaataa accaatcagc tccactgaaa gaattaagag agcacacgaa
     5101 gttactgcac tcataggaat agatactaca cacaaaactt acttatgtca catgcaagat
     5161 gtagacccaa cactttcagt agaatactca gaagctgaat tttgccaaat tcccgaaaga
     5221 acacgaagat caatattagc caactttaga caactctacg aaacacaaga caaccctgag
     5281 agagaggaag atgttgtatc tcaaaatgag atatgtcagt atgacaatac gtcaccctaa
     5341 taagagctct cctagtcgca cgcttgtagt ggatcgtctg cataatatcg tgtgacccgg
     5401 gtgagagaga ggaagatgtt gtatctcaaa atgagatatg tcagtatgac aatacgtcac
     5461 cctgaacgtt cataaaacac atatgaaaca accttataac aaaacgaaca acatgagaca
     5521 aaacccgacc ttccctagct gaactaccca aagtataaat gcctgaacaa ttagtttaga
     5581 tccgagattc cgcgcttcca ccacttagta tgattcatat tttatataat atataagata
     5641 agtaacattc cgtgaattaa tctgataaac tgttttgaca actggttact tccctaagac
     5701 tgtttatatt aggattgtca agacactccg gtattactcg agcccgtaat acaacaggcg
     5761 gccgcgaatt cctttctgca tgccacgttc gcggcgcctg atgcggtatt ttctccttac
     5821 gcatctgtgc ggtatttcac accgcatata tcgctgggcc attctcatga agaatatctt
     5881 gaatttattg tcatattact agttggtgtg gaagtccata tatcggtgat caatatagtg
     5941 gttgacatgc tggctagtca acattgagcc ttttgatcat gcaaatatat tacggtattt
     6001 tacaatcaaa tatcaaactt aactattgac tttataactt atttaggtgg taacattctt
     6061 ataaaaaaga aaaaaattac tgcaaaacag tactagcttt taacttgtat cctaggttat
     6121 ctatgctgtc tcaccataga gaatattacc tatttcagaa tgtatgtcca tgattcgccg
     6181 ggtaaataca tataatacac aaatctggct taataaagtc tataatatat ctcataaaga
     6241 agtgctaaat tggctagtgc tatatatttt taagaaaatt tcttttgact aagtccatat
     6301 cgactttgta aaagttcact ttagcataca tatattacac gagccagaaa ttgtaacttt
     6361 tgcctaaaat cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca
     6421 acttttttaa aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat
     6481 ttatttattg tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg
     6541 tccttggaaa aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa
     6601 ctctttacag aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt
     6661 ttatcgtcac agttttacag taaataagta tcacctctta gagttcgatg ataagctgtc
     6721 aaacatgaga attaattcca catgttaaaa tagtgaagga gcatgttcgg cacacagtgg
     6781 accgaacgtg gggtaagtgc actagggtcc ggttaaacgg atctcgcatt gatgaggcaa
     6841 cgctaattat caacatatag attgttatct atctgcatga acacgaaatc tttacttgac
     6901 gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg tttaatatgt gtcactgttt
     6961 gatattactg tcagcgtaga agataatagt aaaagcggtt aataagtgta tttgagataa
     7021 gtgtgataaa gtttttacag cgaaaagacg ataaatacaa gaaaatgatt acgaggatac
     7081 ggagagaggt atgtacatgt gtatttatat actaagctgc cggcggttgt ttgcaagacc
     7141 gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac attctcttca
     7201 acaagtttga ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat gtatattttc
     7261 ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc tttacatctt gttattttac
     7321 agattttatg tttagatctt ttatgcttgc ttttcaaaag gcttgcaggc aagtgcacaa
     7381 acaatactta aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt
     7441 tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc
     7501 aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa
     7561 cataaaatgt aagctctcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca
     7621 atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg
     7681 tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat
     7741 aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac
     7801 gatcgatgat aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag
     7861 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     7921 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     7981 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     8041 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     8101 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     8161 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     8221 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     8281 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     8341 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     8401 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     8461 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     8521 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     8581 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     8641 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     8701 tagctttcga catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta
     8761 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
     8821 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     8881 aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta
     8941 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     9001 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9061 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9121 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9181 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9241 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9301 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9361 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9421 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9481 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9541 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9601 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9661 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9721 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9781 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9841 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9901 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9961 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10021 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10081 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10321 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10921 gcagcgagtc agtgagcgag gaagcggaag a
//
