LOCUS       sequence-214-exp        3700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3829
FEATURES             Location/Qualifiers
     source          1..3700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(155..183)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     931..951
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1089..1106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1089..1105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1098..1120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1314..1333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1433..1455
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1493..1511)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1579..1683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1684..1752,1753..2544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1902..1921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2715..3303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3204..3223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3457..3474
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3591..3612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3627..3632,3633..3650,3651..3657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3665..3681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3670..3692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3689..3700,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3689..3700,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttacatgtc ctgggcccat cgattcggac gtcccgaggg
       61 cttaagcgga cgcgtcgagg ggatccccgg gtaccgagct cgaattcaag taccggtaga
      121 aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      181 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      241 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag
      301 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac
      361 tactttctgt tatggtgttc aatgcttctc gagataccca gatcatatga aacggcatga
      421 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      481 tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt
      541 taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag
      721 tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag
      781 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      841 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
      901 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
      961 gattacacat ggcatggacg aactatacaa aagcccacaa gcttacatgt cctgggccca
     1021 tcgattcgga cgtcccgagg gcttaagcgg acgcgtcgag gggatccccg ggtaccgagc
     1081 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     1141 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     1201 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     1261 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     1321 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     1381 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     1441 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     1501 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1561 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1621 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1681 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1741 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1801 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1861 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1921 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1981 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2041 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2101 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2161 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2221 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2281 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2341 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2401 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2461 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2521 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2821 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3421 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3481 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     3601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     3661 ggaattgtga gcggataaca atttcacaca ggaaacagct
//
