LOCUS       sequence-214456-        5993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CH192: PIq:luxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Chen Y, Ho JML, Shis DL, Gupta C, Long J, Wagner DS, Ott W, Josic K,
            Bennett MR
  TITLE     Tuning the dynamic range of bacterial promoters regulated by 
            ligand-inducible transcription factors.
  JOURNAL   Nat Commun. 2018 Jan 4;9(1):64. doi: 10.1038/s41467-017-02473-5.
  PUBMED    29302024
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-4"; volume: "9(1):64. doi"; pages: " 
            10.1038/s41467-017-02473-5"
COMMENT     Sequence Label: CH192
FEATURES             Location/Qualifiers
     source          1..5993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      98..125
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(735..1394)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1309..1328
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1395..1497)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    1554..1587
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(1724..1751)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2904..2933)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3124..3146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3135..3151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3299..3530
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      3565..3651
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4189..5139)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5187..5409
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5911..5945)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gaagttccta ttctctagaa agtataggaa cttcaacgcc atgagcggcc tcatttctta
       61 ttctgagtta caacagtccg caccgctgtc tcacactggc tcaccttcgg gtgggccttt
      121 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
      181 tatttcctag cggccgcagg cggtagctcc ttccggtggg cgcggggcat gactatcgtc
      241 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc
      301 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc
      361 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
      421 gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat
      481 cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc
      541 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
      601 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
      661 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
      721 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      781 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
      841 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      901 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
      961 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1021 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1081 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1141 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     1201 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1261 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1321 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1381 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1441 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     1501 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accgaagttc
     1561 ctattctcta gaaagtatag gaacttctgc tcatgtttga cagcttatca tcgatgcata
     1621 atgtgcctgt caaatggacg aagcagggat tctgctagct aggaaataat aaaaaagccg
     1681 gattaataat ctggcttttt atattctctc tctagtatat aaacgcagaa aggcccaccc
     1741 gaaggtgagc cagtgtgaga cagcggtgcg gactgttgta actcagaata agaaatgagg
     1801 ccgctcatgg cgttgactct cagtcatagt atcgtggtat caccggttgg ttccactctc
     1861 tgttgcgggc aacttcagca gcacgtaggg gaagcttatt aatttttaaa gtatgggcaa
     1921 tcaattgctc ctgttaaaat tgctttagaa atactttggc agcggtttgt tgtattgagt
     1981 ttcatttgcg cattggttaa atggaaagtg acagtacgct cactgcagcc taatattttt
     2041 gaaatatccc aagagctttt tccttcgcag gcccacgcta aacattcttt ttctcttttg
     2101 gttaaatcgt tgtttgattt attatttgct atatttattt ttcgataatt atcaactaga
     2161 gaaggaacaa ttaatggtat gttcatacac gcatgtaaaa ataaactatc tatatagttg
     2221 tctttttctg aatgtgcaaa actaagcatt ccgaagccat tgttagccgt atgaataggg
     2281 aaactaaacc cagtgataag acctgatgtt ttcgcttctt taattacatt tggagatttt
     2341 ttatttacag cattgttttc aaatatattc caattaattg gtgaatgatt ggagttagaa
     2401 taatctacta taggatcata ttttattaaa ttagcgtcat cataatattg cctccatttt
     2461 ttagggtaat tatctagaat tgaaatatca gatttaacca tagaatgagg ataaatgatc
     2521 gcgagtaaat aatattcaca atgtaccatt ttagtcatat cagataagca ttgattaata
     2581 tcattattgc ttctacatgc tttaatttta ttaattattc tgtatgtgtc gtcggcattt
     2641 atgtttttca tactagaatt tctccttagc atgattaaga tgtttcagta cgaaaattgc
     2701 tttcattgtt gatctccttt ttaagtggca tgcttccggg cgctatcatg ccataccgcg
     2761 aaaggttttg caccatcgac agttcatagg tgattgctca ggacatttct gttagaagga
     2821 atcgttttcc ttacttttcc ttacgcacaa gagttccgta gctgttcaag tttgtgtttc
     2881 aactgttctc gtcgtttccg caacaagtcc tcttcagaaa tgagcttttg ctcctctgct
     2941 tggacggaca ggatgtatgc tgtggctttt ttaaggataa ctaccttggg ggccttttca
     3001 ttgttttcca actccgggat ctggtcacgc agggcaaaaa agctccgttt tagctcgttc
     3061 ctcctctggc gctccaagac gttgtgtgtt cgcctcttga cattctcctc ggtgtccgag
     3121 ggccctgtgt gaaattgtta tccgctcaca attccacaca gacgtcggaa ttgccagctg
     3181 gggcgccctc tggtaaggtt gggaagctcg agcgcactga gctcctagag tgcgcgacag
     3241 gtttgatgac aaaaaattag cgcaagaaga caaaaatcac cttgcgctaa tgctctgtta
     3301 caggtcacta ataccatcta agtagttgat tcatagtgac tgcatatgtt gtgttttaca
     3361 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     3421 ttacgtttct cgttcagctt ttttatacta agttggcatt ataaaaaagc attgcttatc
     3481 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gatttcaatt
     3541 ttgtcccact ccccctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3601 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
     3661 tagacctagg gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt
     3721 tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag
     3781 aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt
     3841 gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca
     3901 agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt
     3961 ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct
     4021 ttaaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca
     4081 tctgtgcata tggacagttt tccctttgat atctaacggt gaacagttgt tctacttttg
     4141 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc
     4201 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga
     4261 accattgaga tcatgcttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag
     4321 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttac gtaggtagga
     4381 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag
     4441 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg
     4501 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat
     4561 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa
     4621 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt
     4681 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac
     4741 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc
     4801 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat
     4861 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt
     4921 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg
     4981 gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag
     5041 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat
     5101 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg
     5161 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct
     5221 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc
     5281 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt
     5341 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt
     5401 gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt
     5461 tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc
     5521 gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
     5581 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
     5641 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
     5701 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     5761 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     5821 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
     5881 ttactgtccc tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     5941 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gag
//
