LOCUS       sequence-214607-        1758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically-encoded ratiometric bioluminescent Ca2+ indicator based 
            on GCaMP6s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Qian Y, Rancic V, Wu J, Ballanyi K, Campbell RE
  TITLE     A bioluminescent Ca2+ indicator based on a topological variant of 
            GCaMP6s.
  JOURNAL   Chembiochem. 2018 Jun 22. doi: 10.1002/cbic.201800255.
  PUBMED    29934970
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2018 Jun 22. doi: 10.1002/cbic.201800255."
COMMENT     Sequence Label: pcDNA-LUCI-GECO1
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..513
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(559..580)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(820..839)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1423..1479
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     1692..1713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
       61 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
      121 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
      181 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
      241 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
      301 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
      361 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
      421 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
      481 accggctggc ggctgtgcga acgcattctg gcgcggaaga gcaagggcga ggagctgttc
      541 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      601 gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      661 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      721 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      781 cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      841 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      901 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct gccggaccaa
      961 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg
     1021 gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc
     1081 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc
     1141 gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga cacggaagaa
     1201 gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat cagtgcagca
     1261 gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa
     1321 atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa
     1381 atgatgacag cgaagggtgg cggaggttct gtcgactcat cacgtcgtaa gtggaataag
     1441 acaggtcacg cagtcagagc tataggtcgg ctgagctcac tcgagaacgt ctatatcaag
     1501 gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa catcgaggac
     1561 ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1621 ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag
     1681 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1741 gacgagctgt acaagtaa
//
