LOCUS       sequence-21468-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TLR3-CFP
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(2..712,713..715,716..718))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(44..65)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     393..412
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     652..673
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
       61 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
      121 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      181 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagtgggcc ttgatgccgt
      241 tcttctgctt gtcggcggtg atatagacgt tgtggctgat gtagttgtac tccagcttgt
      301 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      361 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      421 agatggtacg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      481 tcatgtggtc ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg
      541 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
      601 aggtggcatc gccctcgccc tcgccggaca cgctgaacct gtggccgttt acgtcgccgt
      661 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatct
      721 cgagatgtac agagtttttg gatccaagtg ctacttgcaa tttatgacga aaggcaccta
      781 tccgttcttt ctgaactggc cagttcaaga tgcagtgaga tttaaacatt cctcttcgca
      841 aacagagtgc atggttcagt ttataatctg gaatctcctc aaggaaaacc aatat
//
