LOCUS       sequence-214872-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; wnt promoter-driven expression of iRFP; 
            MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_TOP-iRFP_L5-L4
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(914..934)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gccggcagcg acaccttccc gaactcaccg gactggcgat cggcgccctg atcggccgct
       61 ctgcggccga tgtcttcgac tcggagacgc acaaccgtct gacgatcgcc ttggccgagc
      121 ccggggcggc cgtcggagca ccgatcactg tcggcttcac gatgcgaaag gacgcaggct
      181 tcatcggctc ctggcatcgc catgatcagc tcatcttcct cgagctcgag cctccccagc
      241 gggacgtcgc cgagccgcag gcgttcttcc gccgcaccaa cagcgccatc cgccgcctgc
      301 aggccgccga aaccttggaa agcgcctgcg ccgccgcggc gcaagaggtg cggaagatta
      361 ccggcttcga tcgggtgatg atctatcgct tcgcctccga cttcagcggc gaagtgatcg
      421 cagaggatcg gtgcgccgag gtcgagtcaa aactaggcct gcactatcct gcctcaaccg
      481 tgccggcgca ggcccgtcgg ctctatacca tcaacccggt acggatcatt cccgatatca
      541 attatcggcc ggtgccggtc accccagacc tcaatccggt caccgggcgg ccgattgatc
      601 ttagcttcgc catcctgcgc agcgtctcgc ccgtccatct ggaattcatg cgcaacatag
      661 gcatgcacgg cacgatgtcg atctcgattt tgcgcggcga gcgactgtgg ggattgatcg
      721 tttgccatca ccgaacgccg tactacgtcg atctcgatgg ccgccaagcc tgcgagctag
      781 tcgcccaggt tctggcctgg cagatcggcg tgatggaaga gtgagtcact agnnaacggg
      841 ccgccagtgt gnctggaatt ctgcagatat ccatcacact ggncggccgc tcgagcatgc
      901 atctagaggc acccaacttt tctatacaaa gttggcatta taagaaagca ttgcttawtc
      961 aatttgttgc a
//
