LOCUS       sequence-214873-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single pathway reporter; wnt promoter-driven expression of iRFP; 
            MultiSite Gateway entry vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: pMuLE_ENTR_TOP-iRFP_L5-L4
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..90
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        265..296
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtat acaaaagttg ggagacccaa gcttggtacc gagctcttac
      121 gcgagatcaa agggggtaag atcaaagggg gtaagatcaa aggggcgcga gatcaaaggg
      181 ggtaagatca aagggggtaa gatcaaaggg ggtaagatca aaggggcgcg cccgcgtgct
      241 agcccgggct cgagatctag actctagagg gtatataatg gaagctcgaa ttccagcttg
      301 gcattccggt actgttggta aaaagcttgg cattccggta ctgttggtaa agccaccatc
      361 agctgcacca tggcggaagg atccgtcgcc aggcagcctg acctcttgac ctgcgacgat
      421 gagccgatcc atatccccgg tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc
      481 gacatgacga tcgttgccgg cagcgacaac cttcccgaac tcaccggact ggcgatcggc
      541 gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg
      601 atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg
      661 cgaaaggacg caggcttcat cggctcctgg catcgccatg atcagctcat cttcctcgag
      721 ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc
      781 gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa
      841 gaggtgcgga agattaccgg cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc
      901 agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg agtcaaaact aggcctgcac
      961 tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg
     1021 atcattcccg atatcaatta tcgggccggt gccggtcacc ccagacctca atccggtcac
     1081 cgggcggccg attgatctta gc
//
