LOCUS       sequence-214918-       10037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RGS component of opto-RGS2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Hannanta-Anan P, Chow BY
  TITLE     Optogenetic Inhibition of Galphaq Protein Signaling Reduces Calcium 
            Oscillation Stochasticity.
  JOURNAL   ACS Synth Biol. 2018 Jun 15;7(6):1488-1495. doi: 
            10.1021/acssynbio.8b00065. Epub 2018 Jun 4.
  PUBMED    29792810
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00065"; journalName: "ACS Synth Biol"; date: 
            "2018-06-15- 15"; volume: "7"; issue: "6"; pages: "1488-1495"
COMMENT     Sequence Label: pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box
FEATURES             Location/Qualifiers
     source          1..10037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      232..820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     721..740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     974..991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1108..1129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1144..1149,1150..1167,1168..1174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1182..1198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1187..1209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1206..1222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1206..1222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1241..1261
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1243..1261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1289..1515
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1516..1696
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1743..1868
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2361..2594
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2779..2823
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     3121..3141
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3430..3633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3584..3604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3672..5165
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      5184..5193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5190..5897
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5335..5353)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5603..5622
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6419..6536
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6585..7095
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7014..7032
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7117..7716
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7117..7136)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7613..7633
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7844..8077
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8149..8270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8186..8205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8240..8259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8310..8445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8465..8484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8466..8484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8494..8511)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8494..8510)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8503..8525)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8652..9107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8739..8758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8949..8970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9133..9237
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9238..9306,9307..10037,1..61)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9456..9475)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
       61 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      121 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      181 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      361 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      601 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      781 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      841 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      901 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      961 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1021 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1081 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1141 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1201 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     1261 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     1321 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     1381 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     1441 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     1501 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     1561 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     1621 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     1681 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     1741 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     1801 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     1861 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     1921 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     1981 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     2041 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     2101 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     2161 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     2221 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     2281 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     2341 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     2401 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     2461 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     2521 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     2581 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     2641 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     2701 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     2761 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     2821 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     2881 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     2941 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     3001 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     3061 gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc ccccttcacc
     3121 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     3181 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     3241 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     3301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     3361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     3421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     3481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     3541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     3601 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
     3661 agctagccac catgaagatg gacaaaaaga ctatagtttg gtttagaaga gacctaagga
     3721 ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt cctgtcttca
     3781 tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga tggtggatga
     3841 aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac ctcactttaa
     3901 tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc ggtgctacaa
     3961 aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat accgtaaagg
     4021 agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta ttgtatgaac
     4081 cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct tactggaaga
     4141 aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg ttgatgccaa
     4201 taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg ctggagaatg
     4261 aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga tggagcaatg
     4321 ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca aagaacagca
     4381 agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc ggggaaataa
     4441 gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca agagataaga
     4501 acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta agagagtatt
     4561 ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg agtcatcttc
     4621 ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa ggcaggaccg
     4681 gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg atgcataaca
     4741 gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca tggaaatggg
     4801 gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac atccttggct
     4861 ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg gacaatcccg
     4921 cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg cttcccgagc
     4981 ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta accgtactca
     5041 aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac atcgacacag
     5101 ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc atgatcggag
     5161 cagcagcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     5221 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     5281 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     5341 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     5401 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     5461 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     5521 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     5581 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     5641 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     5701 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     5761 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     5821 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     5881 tggacgagct gtacaagtgc ggccgcggcg gaggtggttc tggcggtgga ggttcaggcg
     5941 gtggtggaag tgagctcact agttccggac tgtggtcaga agcatttgac gagctgctag
     6001 ccagcaaata tggtcttgct gcattcaggg cttttttaaa gtcggaattc tgtgaagaaa
     6061 atattgaatt ctggctggcc tgtgaagact tcaaaaaaac caaatcaccc caaaagctgt
     6121 cctcaaaagc aaggaaaata tatactgact tcatagaaaa ggaagctcca aaagagataa
     6181 acatagattt tcaaaccaaa actctgattg cccagaatat acaagaagct acaagtggct
     6241 gctttacaac tgcccagaaa agggtataca gcttgatgga gaacaactct tatcctcgtt
     6301 tcttggagtc agaattctac caggacttgt gtaaaaagcc acaaatcacc acagagcctc
     6361 atgctacata agtcgaccaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     6421 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     6481 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     6541 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
     6601 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     6661 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     6721 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     6781 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     6841 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     6901 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     6961 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     7021 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     7081 gacctctctc cccaggggga tccaccggag cttaccatga ccgagtacaa gcccacggtg
     7141 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7201 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7261 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7321 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7381 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7441 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7501 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7561 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7621 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7681 cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
     7741 ccgaccgaaa ggagcgcacg accccatgca tcggtacctt taagaccaat gacttacaag
     7801 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7861 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7921 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7981 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8041 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8101 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8281 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8341 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8401 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     8461 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     8521 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8581 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8641 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8701 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8761 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8821 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8881 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8941 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9001 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9061 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     9121 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9181 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9241 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9301 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9361 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9421 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9481 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9541 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9601 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9661 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9721 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9781 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9841 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9901 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9961 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10021 acggggagtc aggcaac
//
