LOCUS       sequence-215304-       10645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Martin A, Baker TA, Sauer RT
  TITLE     Rebuilt AAA + motors reveal operating principles for ATP-fuelled 
            machines.
  JOURNAL   Nature. 2005 Oct 20. 437(7062):1115-20.
  PUBMED    16237435
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Oct 20. 437(7062):1115-20."
COMMENT     Sequence Label: pACYC-FLAG-dN6-His
FEATURES             Location/Qualifiers
     source          1..10645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(771..1853)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1815..1834
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1854..1931)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1977..1996
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1996..2020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2070..2093
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8946..8963
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8994..9038
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(9076..9094)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9090..9137
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(9358..10017)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9932..9951
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10018..10120)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(10512..10529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
      121 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      181 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      241 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      301 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
      361 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
      421 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
      481 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
      541 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
      601 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag
      661 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
      721 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
      781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      841 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
      901 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
      961 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     1021 tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag cccggactcg
     1081 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     1141 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     1201 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     1261 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     1321 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     1381 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     1441 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     1501 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     1561 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     1621 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     1681 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     1741 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     1801 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     1861 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     1921 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaaattaat
     1981 acgactcact ataggggaat tgtgagcgga taacaattcc cctgtagaaa taattttgtt
     2041 taactttaat aaggagatat accatggccg attacaaaga tgacgatgac aaaagctctc
     2101 atatgagtgc gctaccgacg ccgcatgaaa ttcgcaacca cctggacgat tacgttatcg
     2161 gccaggaaca ggcgaaaaaa gtgctggcgg tcgcggtata caaccattac aaacgtctgc
     2221 gcaacggcga taccagcaat ggcgtcgagt tgggcaaaag taacattctg ctgatcggtc
     2281 cgaccggttc cggtaaaacg ctgctggctg aaacgctggc gcgcctgctg gatgttccgt
     2341 tcaccatggc cgacgcgact acactgaccg aagccggtta tgtgggtgaa gacgttgaaa
     2401 acatcattca gaagctgttg cagaaatgcg actacgatgt ccagaaagca cagcgtggta
     2461 ttgtctacat cgatgaaatc gacaagattt ctcgtaagtc agacaacccg tccattaccc
     2521 gagacgtttc cggtgaaggc gtacagcagg cactgttgaa actgatcgaa ggtacggtag
     2581 ctgctgttcc accgcaaggt gggcgtaaac atccgcagca ggaattcttg caggttgata
     2641 cctctaagat cctgtttatt tgtggcggtg cgtttgccgg tctggataaa gtgatttccc
     2701 accgtgtaga aaccggctcc ggcattggtt ttggcgcgac ggtaaaagcg aagtccgaca
     2761 aagcaagcga aggcgagctg ctggcgcagg ttgaaccgga agatctgatc aagtttggtc
     2821 ttatccctga gtttattggt cgtctgccgg ttgtcgcaac gttgaatgaa ctgagcgaag
     2881 aagctctgat tcagatcctc aaagagccga aaaacgccct gaccaagcag tatcaggcgc
     2941 tgtttaatct ggaaggcgtg gatctggaat tccgtgacga ggcgctggat gctatcgcta
     3001 agaaagcgat ggcgcgtaaa accggtgccc gtggcctgcg ttccatcgta gaagccgcac
     3061 tgctcgatac catgtacgat ctgccgtcca tggaagacgt cgaaaaagtg gttatcgacg
     3121 agtcggtaat tgatggtcaa agcgaaccgt tgctgattta tggcaagccg gaagcgcaac
     3181 aggcatctgg tgaggccagc ggggccggcg gttctgaagg tggcggcagc gaaggtggta
     3241 ccagcggggc cacaagtgcg ctaccgacgc cgcatgaaat tcgcaaccac ctggacgatt
     3301 acgttatcgg ccaggaacag gcgaaaaaag tgctggcggt cgcggtatac aaccattaca
     3361 aacgtctgcg caacggcgat accagcaatg gcgtcgagtt gggcaaaagt aacattctgc
     3421 tgatcggtcc gaccggttcc ggtaaaacgc tgctggctga aacgctggcg cgcctgctgg
     3481 atgttccgtt caccatggcc gacgcgacta cactgaccga agccggttat gtgggtgaag
     3541 acgttgaaaa catcattcag aagctgttgc agaaatgcga ctacgatgtc cagaaagcac
     3601 agcgtggtat tgtctacatc gatgaaatcg acaagatttc tcgtaagtca gacaacccgt
     3661 ccattacccg agacgtttcc ggtgaaggcg tacagcaggc actgttgaaa ctgatcgaag
     3721 gtacggtagc tgctgttcca ccgcaaggtg ggcgtaaaca tccgcagcag gaattcttgc
     3781 aggttgatac ctctaagatc ctgtttattt gtggcggtgc gtttgccggt ctggataaag
     3841 tgatttccca ccgtgtagaa accggctccg gcattggttt tggcgcgacg gtaaaagcga
     3901 agtccgacaa agcaagcgaa ggcgagctgc tggcgcaggt tgaaccggaa gatctgatca
     3961 agtttggtct tatccctgag tttattggtc gtctgccggt tgtcgcaacg ttgaatgaac
     4021 tgagcgaaga agctctgatt cagatcctca aagagccgaa aaacgccctg accaagcagt
     4081 atcaggcgct gtttaatctg gaaggcgtgg atctggaatt ccgtgacgag gcgctggatg
     4141 ctatcgctaa gaaagcgatg gcgcgtaaaa ccggtgcccg tggcctgcgt tccatcgtag
     4201 aagccgcact gctcgatacc atgtacgatc tgccgtccat ggaagacgtc gaaaaagtgg
     4261 ttatcgacga gtcggtaatt gatggtcaaa gcgaaccgtt gctgatttat ggcaagccgg
     4321 aagcgcaaca ggcatctggt gaggccagcg gggccggcgg ttctgaaggt ggcggcagcg
     4381 aaggtggctc gagcggggcc acaagtgcgc taccgacgcc gcatgaaatt cgcaaccacc
     4441 tggacgatta cgttatcggc caggaacagg cgaaaaaagt gctggcggtc gcggtataca
     4501 accattacaa acgtctgcgc aacggcgata ccagcaatgg cgtcgagttg ggcaaaagta
     4561 acattctgct gatcggtccg accggttccg gtaaaacgct gctggctgaa acgctggcgc
     4621 gcctgctgga tgttccgttc accatggccg acgcgactac actgaccgaa gccggttatg
     4681 tgggtgaaga cgttgaaaac atcattcaga agctgttgca gaaatgcgac tacgatgtcc
     4741 agaaagcaca gcgtggtatt gtctacatcg atgaaatcga caagatttct cgtaagtcag
     4801 acaacccgtc cattacccga gacgtttccg gtgaaggcgt acagcaggca ctgttgaaac
     4861 tgatcgaagg tacggtagct gctgttccac cgcaaggtgg gcgtaaacat ccgcagcagg
     4921 aattcttgca ggttgatacc tctaagatcc tgtttatttg tggcggtgcg tttgccggtc
     4981 tggataaagt gatttcccac cgtgtagaaa ccggctccgg cattggtttt ggcgcgacgg
     5041 taaaagcgaa gtccgacaaa gcaagcgaag gcgagctgct ggcgcaggtt gaaccggaag
     5101 atctgatcaa gtttggtctt atccctgagt ttattggtcg tctgccggtt gtcgcaacgt
     5161 tgaatgaact gagcgaagaa gctctgattc agatcctcaa agagccgaaa aacgccctga
     5221 ccaagcagta tcaggcgctg tttaatctgg aaggcgtgga tctggaattc cgtgacgagg
     5281 cgctggatgc tatcgctaag aaagcgatgg cgcgtaaaac cggtgcccgt ggcctgcgtt
     5341 ccatcgtaga agccgcactg ctcgatacca tgtacgatct gccgtccatg gaagacgtcg
     5401 aaaaagtggt tatcgacgag tcggtaattg atggtcaaag cgaaccgttg ctgatttatg
     5461 gcaagccgga agcgcaacag gcatctggtg aggccagcgg ggccggcggt tctgaaggtg
     5521 gcggcagcga aggtgggtcg accggggcca caagtgcgct accgacgccg catgaaattc
     5581 gcaaccacct ggacgattac gttatcggcc aggaacaggc gaaaaaagtg ctggcggtcg
     5641 cggtatacaa ccattacaaa cgtctgcgca acggcgatac cagcaatggc gtcgagttgg
     5701 gcaaaagtaa cattctgctg atcggtccga ccggttccgg taaaacgctg ctggctgaaa
     5761 cgctggcgcg cctgctggat gttccgttca ccatggccga cgcgactaca ctgaccgaag
     5821 ccggttatgt gggtgaagac gttgaaaaca tcattcagaa gctgttgcag aaatgcgact
     5881 acgatgtcca gaaagcacag cgtggtattg tctacatcga tgaaatcgac aagatttctc
     5941 gtaagtcaga caacccgtcc attacccgag acgtttccgg tgaaggcgta cagcaggcac
     6001 tgttgaaact gatcgaaggt acggtagctg ctgttccacc gcaaggtggg cgtaaacatc
     6061 cgcagcagga attcttgcag gttgatacct ctaagatcct gtttatttgt ggcggtgcgt
     6121 ttgccggtct ggataaagtg atttcccacc gtgtagaaac cggctccggc attggttttg
     6181 gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg cgagctgctg gcgcaggttg
     6241 aaccggaaga tctgatcaag tttggtctta tccctgagtt tattggtcgt ctgccggttg
     6301 tcgcaacgtt gaatgaactg agcgaagaag ctctgattca gatcctcaaa gagccgaaaa
     6361 acgccctgac caagcagtat caggcgctgt ttaatctgga aggcgtggat ctggaattcc
     6421 gtgacgaggc gctggatgct atcgctaaga aagcgatggc gcgtaaaacc ggtgcccgtg
     6481 gcctgcgttc catcgtagaa gccgcactgc tcgataccat gtacgatctg ccgtccatgg
     6541 aagacgtcga aaaagtggtt atcgacgagt cggtaattga tggtcaaagc gaaccgttgc
     6601 tgatttatgg caagccggaa gcgcaacagg catctggtga ggccagcggg gccggcggtt
     6661 ctgaaggtgg cggcagcgaa ggtggcacct ctgcaggcac aagtgcgcta ccgacgccgc
     6721 atgaaattcg caaccacctg gacgattacg ttatcggcca ggaacaggcg aaaaaagtgc
     6781 tggcggtcgc ggtatacaac cattacaaac gtctgcgcaa cggcgatacc agcaatggcg
     6841 tcgagttggg caaaagtaac attctgctga tcggtccgac cggttccggt aaaacgctgc
     6901 tggctgaaac gctggcgcgc ctgctggatg ttccgttcac catggccgac gcgactacac
     6961 tgaccgaagc cggttatgtg ggtgaagacg ttgaaaacat cattcagaag ctgttgcaga
     7021 aatgcgacta cgatgtccag aaagcacagc gtggtattgt ctacatcgat gaaatcgaca
     7081 agatttctcg taagtcagac aacccgtcca ttacccgaga cgtttccggt gaaggcgtac
     7141 agcaggcact gttgaaactg atcgaaggta cggtagctgc tgttccaccg caaggtgggc
     7201 gtaaacatcc gcagcaggaa ttcttgcagg ttgatacctc taagatcctg tttatttgtg
     7261 gcggtgcgtt tgccggtctg gataaagtga tttcccaccg tgtagaaacc ggctccggca
     7321 ttggttttgg cgcgacggta aaagcgaagt ccgacaaagc aagcgaaggc gagctgctgg
     7381 cgcaggttga accggaagat ctgatcaagt ttggtcttat ccctgagttt attggtcgtc
     7441 tgccggttgt cgcaacgttg aatgaactga gcgaagaagc tctgattcag atcctcaaag
     7501 agccgaaaaa cgccctgacc aagcagtatc aggcgctgtt taatctggaa ggcgtggatc
     7561 tggaattccg tgacgaggcg ctggatgcta tcgctaagaa agcgatggcg cgtaaaaccg
     7621 gtgcccgtgg cctgcgttcc atcgtagaag ccgcactgct cgataccatg tacgatctgc
     7681 cgtccatgga agacgtcgaa aaagtggtta tcgacgagtc ggtaattgat ggtcaaagcg
     7741 aaccgttgct gatttatggc aagccggaag cgcaacaggc atctggtgag gccagcgggg
     7801 ccggcggttc tgaaggtggc ggcagcgaag gtggatccag cggggccaca agtgcgctac
     7861 cgacgccgca tgaaattcgc aaccacctgg acgattacgt tatcggccag gaacaggcga
     7921 aaaaagtgct ggcggtcgcg gtatacaacc attacaaacg tctgcgcaac ggcgatacca
     7981 gcaatggcgt cgagttgggc aaaagtaaca ttctgctgat cggtccgacc ggttccggta
     8041 aaacgctgct ggctgaaacg ctggcgcgcc tgctggatgt tccgttcacc atggccgacg
     8101 cgactacact gaccgaagcc ggttatgtgg gtgaagacgt tgaaaacatc attcagaagc
     8161 tgttgcagaa atgcgactac gatgtccaga aagcacagcg tggtattgtc tacatcgatg
     8221 aaatcgacaa gatttctcgt aagtcagaca acccgtccat tacccgagac gtttccggtg
     8281 aaggcgtaca gcaggcactg ttgaaactga tcgaaggtac ggtagctgct gttccaccgc
     8341 aaggtgggcg taaacatccg cagcaggaat tcttgcaggt tgatacctct aagatcctgt
     8401 ttatttgtgg cggtgcgttt gccggtctgg ataaagtgat ttcccaccgt gtagaaaccg
     8461 gctccggcat tggttttggc gcgacggtaa aagcgaagtc cgacaaagca agcgaaggcg
     8521 agctgctggc gcaggttgaa ccggaagatc tgatcaagtt tggtcttatc cctgagttta
     8581 ttggtcgtct gccggttgtc gcaacgttga atgaactgag cgaagaagct ctgattcaga
     8641 tcctcaaaga gccgaaaaac gccctgacca agcagtatca ggcgctgttt aatctggaag
     8701 gcgtggatct ggaattccgt gacgaggcgc tggatgctat cgctaagaaa gcgatggcgc
     8761 gtaaaaccgg tgcccgtggc ctgcgttcca tcgtagaagc cgcactgctc gataccatgt
     8821 acgatctgcc gtccatggaa gacgtcgaaa aagtggttat cgacgagtcg gtaattgatg
     8881 gtcaaagcga accgttgctg atttatggca agccggaagc gcaacaggca tctggtgaat
     8941 ctagtcatca ccatcatcac cactaaaagc ttgcggccgc actcgagtct ggtaaagaaa
     9001 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagcgca gcttaattaa
     9061 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     9121 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
     9181 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
     9241 ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc
     9301 acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaatta
     9361 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     9421 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     9481 ttgcgtataa tatttgccca tagtgaaaac gggggcgaag aagttgtcca tattggccac
     9541 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     9601 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     9661 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     9721 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     9781 gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa gaatgtgaat
     9841 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     9901 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     9961 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
    10021 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
    10081 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa
    10141 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
    10201 atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg ccaacttact
    10261 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
    10321 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
    10381 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
    10441 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
    10501 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
    10561 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
    10621 gtgagagggc cgcggcaaag ccgtt
//
