LOCUS       sequence-215310-        9671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal-Estradiol transcription activation expression cassette marked 
            with KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Costa EA, Subramanian K, Nunnari J, Weissman JS
  TITLE     Defining the physiological role of SRP in protein-targeting 
            efficiency and specificity.
  JOURNAL   Science. 2018 Jan 18. pii: science.aar3607. doi: 
            10.1126/science.aar3607.
  PUBMED    29348368
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Jan 18. pii: science.aar3607. doi: 10.1126/science.aar3607."
COMMENT     Sequence Label: pJW1663
FEATURES             Location/Qualifiers
     source          1..9671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     gene            196..1552
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        196..539
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             540..1349
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(607..626)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1355..1552
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      complement(1633..2088)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1770..1791)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1982..2001
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2214..2236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2228..2245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2229..2245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2252..2271
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2252..2270
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2303..2319
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2303..2319
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(2347..2512)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2433..2457
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(2533..3249)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(2536..3249)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(3274..3294)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(3301..3342)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(3348..3789)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(3354..3377)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    3672..3789
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(3985..4001)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3986..4002)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(4030..4048)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4069..4085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4069..4085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4082..4104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4093..4109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4117..4123,4124..4141,4142..4147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4162..4183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4300..4317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4471..5059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4551..4570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5230..6021,6022..6090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5853..5872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6091..6195)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6302..6320
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    6889..6907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6910..6928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6981..7421
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECCYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLGMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             7467..8402
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             7515..8405
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaatgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
      241 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
      301 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
      361 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
      421 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
      481 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
      541 tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt
      601 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
      661 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      721 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
      781 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
      841 gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      901 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
      961 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     1021 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1081 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1141 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1201 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     1261 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1321 ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt
     1381 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     1441 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     1501 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     1561 tcgatactaa cgccgccatc catgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1621 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     1681 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     1741 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     1801 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     1861 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     1921 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     1981 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2041 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     2101 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2161 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2221 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga
     2281 gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc agggtgtggt
     2341 caataagagc gacctcatac tatacctgag aaagcaacct gacctacagg aaagagttac
     2401 tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta
     2461 ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag
     2521 aagtggcgcg ccttatttgt acaattcatc cataccatgg gtaataccag cagcagtaac
     2581 aaattctaac aagaccatgt ggtctctctt ttcgtttgga tctttggata aggcagattg
     2641 agtggataag taatggttgt ctggtaacaa gactggacca tcaccaattg gagtattttg
     2701 ttgataatgg tcagctaatt gaacagaacc atcttcaatg ttgtgtctaa ttttgaagtt
     2761 aactttgata ccattctttt gtttgtcagc catgatgtaa acattgtgag agttatagtt
     2821 gtattccaat ttgtgaccta aaatgttacc atcttcttta aaatcaatac cttttaattc
     2881 gattctatta actaaggtat caccttcaaa cttgacttca gctctggtct tgtagttacc
     2941 gtcatctttg aaaaaaatag ttctttcttg aacataacct tctggcatgg cagacttgaa
     3001 aaagtcatgt tgtttcatat gatctgggta tctagaaaaa cattgaacac cataagttaa
     3061 agtagtgact aaggttggcc atggaactgg caatttacca gtagtacaaa taaattttaa
     3121 ggtcaattta ccgtaagtag catcaccttc accttcaccg gagacagaaa atttgtgacc
     3181 attaacatca ccatctaatt caaccaaaat tgggacaaca ccagtgaata attcttcacc
     3241 tttagacatg ttaattaaac cagcaccgtc acctccttga aaatacaaat tttctgaacc
     3301 cgtagaatcg agaccgagga gagggttagg gataggctta ccgaattctc cttgacgtta
     3361 aagtatagag gtatattaac aattttttgt tgatactttt atgacatttg aataagaagt
     3421 aatacaaact gaaaatgttg aaagtattag ttaaagtggt tatgcagctt ttccatttat
     3481 atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag aaacaaggct
     3541 aaaaaactaa tcgcattatc atcctatggt tgttaatttg attcgttaat ttgaaggttt
     3601 gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa
     3661 tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt
     3721 gaagacgagg acgcacggag gagagtcttc cgtcggaggg ctgtcgcccg ctcggcggct
     3781 tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa gttccaaaga
     3841 gaaggttttt ttaggctaag ataatggggc tctttacatt tccacaacat ataagtaaga
     3901 ttagatatgg atatgtatat ggtggtaatg ccatgtaata tgattattaa acttctttgc
     3961 gtccatccaa gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccag
     4021 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     4081 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4141 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4201 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5221 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5281 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5341 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5401 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5461 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5521 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5581 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5641 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5701 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5761 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5821 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5881 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5941 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6001 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6061 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtccttta cgaggcgcgt
     6241 gtaagttaca ggcaagcgat ccgtctaaga aaccattatt atcatgacat taacctataa
     6301 aaataggcgt atcacgaggc cctttcgtcg gcaaccaaac ccatacatcg ggattcctat
     6361 aataccttcg ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag
     6421 ataccagaca agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg
     6481 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
     6541 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
     6601 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
     6661 aaatgatgga agacactaaa ggaaaaattt aacgacaaag acagcaccaa cagatgtcgt
     6721 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
     6781 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
     6841 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgaatc cctatcagtg
     6901 atagagatct ccctatcagt gatagagatt cttccttgtt tctttttctg caggtcgaca
     6961 aacaaatgct cgacataaaa atgaagctac tgtcttctat cgaacaagca tgcgatattt
     7021 gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga
     7081 acaactggga gtgttgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc
     7141 tgacagaagt ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc
     7201 gagaagacct tggcatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa
     7261 caggattatt tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag
     7321 tggagactga tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg
     7381 aagagagtag taacaaaggt caaagacagt tgactgtatc gattgactcg gcaggatccg
     7441 gtgacggtgc tggtttaatt aactctgctg gagacatgag agctgccaac ctttggccaa
     7501 gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc
     7561 agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta
     7621 ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc
     7681 tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg
     7741 atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc
     7801 gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc
     7861 agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc
     7921 ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc
     7981 ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc
     8041 atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag
     8101 gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca
     8161 tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg
     8221 tgcccctcta tgacctgctg ctggagatgc tggacgccca ccgcctacat gcgcccacta
     8281 gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta
     8341 cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt ttccctgcca
     8401 cagtcgcggc cgcaggtgac ggtgctggtt taattaacat gacggtcgac catgatttca
     8461 atagcgaaga tattttattc cccatagaaa gcatgagtag tatacaatac gtggagaata
     8521 ataacccaaa taatattaac aacgatgtta tcccgtattc tctagatatc aaaaacactg
     8581 tcttagatag tgcggatctc aatgacattc aaaatcaaga aacttcactg aatttggggc
     8641 ttcctccact atctttcgac tctccactgc ccgtaacgga aacgatacca tccactaccg
     8701 ataacagctt gcatttgaaa gctgatagca acaaaaatcg cgatgcaaga actattgaaa
     8761 atgatagtga aattaagagt actaataatg ctagtggctc tggggcaaat caatacacaa
     8821 ctcttacttc accttatcct atgaacgaca ttttgtacaa catgaacaat ccgttacaat
     8881 caccgtcacc ttcatcggta cctcaaaatc cgactataaa tcctcccata aatacagcaa
     8941 gtaacgaaac taatttatcg cctcaaactt caaatggtaa tgaaactctt atatctcctc
     9001 gagcccaaca acatacgtcc attaaagata atcgtctgtc cttacctaat ggtgctaatt
     9061 cgaatctttt cattgacact aacccaaaca atttgaacga aaaactaaga aatcaattga
     9121 actcagatac aaattcatat tctaactcca tttctaattc aaactccaat tctacgggta
     9181 atttaaattc cagttatttt aattcactga acatagactc catgctagat gattacgttt
     9241 ctagtgatct cttattgaat gatgatgatg atgacactaa tttatcacgc cgaagattta
     9301 gcgacgttat aacaaaccaa gagctctaag caaatagcta aattatatac gaattaatat
     9361 tatgattaag tgtttacgtg agtgcgatat ttttattact atcttataca gttgtatata
     9421 ctctataaaa tgagttgtct attaattaac gcgatgaatg ctttctgggt ttacctctcc
     9481 aacaactcta gtttacttct caatacattc aattgtattt gatttgtcaa tacttcatca
     9541 ttaatcaatt ctatagtttt gtttttctcg tttatttcca aatttaatgc atcaatttta
     9601 ttattcaatt tgtcgttgat tttggttaat gattttatgg tttgatctct ggcattgatt
     9661 gtttgtgtta g
//
