LOCUS       sequence-215340-        8324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chemical induction of MAD1 localisation to a specific site in the 
            cell (by rapamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Kuijt TE, Omerzu M, Saurin AT, Kops GJ
  TITLE     Conditional targeting of MAD1 to kinetochores is sufficient to 
            reactivate the spindle assembly checkpoint in metaphase.
  JOURNAL   Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. 
            Epub 2014 Apr 4.
  PUBMED    24695965
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00412-014-0458-9"; journalName: "Chromosoma"; date: 
            "2014-10"; volume: "123"; issue: "5"; pages: "471-80"
COMMENT     Sequence Label: pcDNA5-eYFP-FKBP-MAD1-WT
FEATURES             Location/Qualifiers
     source          1..8324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(935..937,938..940,941..1651)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(980..1001)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1241..1260)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1588..1609
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1697..1717
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1721..2041
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(4277..4294)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4283..4507
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4640..4659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4790..4837
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4845..5867
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4845..5867
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5997..6118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6034..6053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6088..6107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6167..6183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6167..6183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6180..6202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6191..6207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6215..6221,6222..6239,6240..6245))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6260..6281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6398..6415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6569..7157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6649..6668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7328..8119,8120..8188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7951..7970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8189..8293)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatggtg agcaagggcg aggagctgtt
      961 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1021 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1081 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     1141 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1201 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1261 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1321 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1381 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1441 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1501 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     1561 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1621 gatcactctc ggcatggacg agctgtacaa gtccggaggg gatcggtgga gctccaccgg
     1681 tggcggccgc tctagagaga atctttattt tcagggcgcc ggagtgcagg tggagactat
     1741 ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgcgtgg tgcactacac
     1801 cgggatgctt gaagatggaa agaaagttga ttcctcccgg gacagaaaca agccctttaa
     1861 gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg cccagatgag
     1921 tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg ccactgggca
     1981 cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc taaaactgga
     2041 aagcttggga ggcggtagta tggaagacct gggggaaaac accatggttt tatccaccct
     2101 gagatctttg aacaacttca tctctcagcg tgtggaggga ggctctggac tggatatttc
     2161 tacctcggcc ccaggttctc tgcagatgca gtaccagcag agcatgcagc tggaggaaag
     2221 agcagagcag atccgttcga agtcccacct catccaggtg gagcgggaga aaatgcagat
     2281 ggagctgagt cacaagaggg ctcgagtgga gctggagaga gcagccagca ccagtgccag
     2341 gaactacgag cgtgaggtcg accgcaacca ggagctcctg acgcgcatcc ggcagcttca
     2401 ggagcgggag gccggggcgg aggagaagat gcaggagcag ctggagcgca acaggcagtg
     2461 tcagcagaac ttggatgctg ccagcaagag gctgcgtgag aaagaggaca gtctggccca
     2521 ggctggcgag accatcaacg cactgaaggg gaggatctcg gaactgcagt ggagcgtgat
     2581 ggaccaggag atgcgggtga agcgcctgga gtcggagaag caggagctgc aggagcagct
     2641 ggacctgcaa cacaaaaaat gccaggaagc caatcagaaa atccaggaac tccaggccag
     2701 ccaagaagca agagcagacc acgagcagca gattaaggat ctggagcaga agctgtccct
     2761 gcaagagcag gatgcagcga ttgtgaagaa catgaagtct gagctggtac ggctccctag
     2821 gctggaacgg gagctgaagc agctgcggga ggagagcgcg cacctgcggg agatgagaga
     2881 gaccaacggg ctgctccagg aagagctgga agggctgcag aggaagctgg ggcgccagga
     2941 gaagatgcag gagacgctgg ttggcttgga gctggagaac gagaggctgc tggccaagct
     3001 gcaaagctgg gagagactgg accagaccat gggcctgagc atcaggactc cagaagacct
     3061 ttccagattc gtggttgagc tgcagcagag ggagcttgcc ttgaaggaca agaacagcgc
     3121 cgtcaccagc agcgcccggg ggctggagaa ggccaggcag cagctgcagg aggagctccg
     3181 gcaggtcagc ggccagctgt tggaggagag gaagaagcgc gagacccacg aggcgctggc
     3241 ccggaggctc cagaaacggg tcctgctgct caccaaggag cgggacggta tgcgggccat
     3301 cctggggtcc tacgacagcg agctgacccc ggccgagtac tcaccccagc tgacgcggcg
     3361 catgcgggag gctgaggata tggtgcagaa ggtgcacagc cacagcgccg agatggaggc
     3421 tcagctgtcg caggccctgg aggagctggg aggccagaaa caaagagcag acatgctgga
     3481 gatggagctg aagatgctga agtctcagtc cagctctgcc gaacagagct tcctgttctc
     3541 cagggaggag gcggacacgc tcaggttgaa ggtcgaggag ctggaaggcg agcggagtcg
     3601 gctggaggag gaaaagagga tgctggaggc acagctggag cggcgagctc tgcagggtga
     3661 ctatgaccag agcaggacca aagtgctgca catgagcctg aaccccacca gtgtggccag
     3721 gcagcgcctg cgcgaggacc acagccagct gcaggcggag tgcgagcgac tgcgcgggct
     3781 cctgcgcgcc atggagagag gaggcaccgt cccagccgac cttgaggctg ccgccgcgag
     3841 tctgccatcg tccaaggagg tggcagagct gaagaagcag gtggagagtg ccgagctgaa
     3901 gaaccagcgg ctcaaggagg ttttccagac caagatccag gagttccgca aggcctgcta
     3961 cacgctcacc ggctaccaga tcgacatcac cacggagaac cagtaccggc tgacctcgct
     4021 gtacgccgag cacccaggcg actgcctcat cttcaaggcc accagcccct cgggttccaa
     4081 gatgcagcta ctggagacag agttctcaca caccgtgggc gagctcatcg aggtgcacct
     4141 gcggcgccag gacagcatcc ctgccttcct cagctcgctc accctcgagc tcttcagccg
     4201 ccagaccgtg gcataagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     4261 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4321 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4381 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4441 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4501 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     4561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4681 ggctttcccc gtcaagctct aaatcggggg tccctttagg gttccgattt agtgctttac
     4741 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat
     4801 tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca
     4861 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     4921 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     4981 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     5041 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     5101 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     5161 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     5221 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     5281 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     5341 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     5401 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     5461 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     5521 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     5581 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     5641 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     5701 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     5761 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     5821 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag
     5881 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5941 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6121 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6241 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
