LOCUS       sequence-215572-        8890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized 
            AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS
            and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xH
            A (AAS1060)
FEATURES             Location/Qualifiers
     source          1..8890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1769..5689
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNVEKNRGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5409..5418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5690..5737
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5744..5824
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5914..5933)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5979..6034
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6033..6052)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6393..6409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6393..6409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6406..6428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6417..6433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6441..6447,6448..6465,6466..6471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6486..6507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6566..6761
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6612..6747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6767..6901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6804..6823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6858..6877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6969..6986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7140..7728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7220..7239)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7899..8690,8691..8759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8522..8541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8760..8864)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gacacagttc gagggcttta ccaacctgta
     1801 tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat
     1861 ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa
     1921 gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct
     1981 ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac
     2041 aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat
     2101 cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg
     2161 cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac
     2221 aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg
     2281 cttttataga aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca
     2341 ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct
     2401 gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat
     2461 cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac
     2521 acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac
     2581 cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac
     2641 agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc
     2701 cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca
     2761 gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc
     2821 cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct
     2881 ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga
     2941 gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg
     3001 cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct
     3061 gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga
     3121 tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct
     3181 ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga
     3241 ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct
     3301 gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt
     3361 caagctgaac tttcagatgc ctacactggc cagaggctgg gacgtgaatg tggagaagaa
     3421 cagaggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca
     3481 gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg agggctttga
     3541 taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt gcagcaccca
     3601 gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc tgtccaacaa
     3661 tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga
     3721 gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg gctacagaga
     3781 ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac
     3841 ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta
     3901 tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat
     3961 catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc
     4021 caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc tgttttctcc
     4081 agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc
     4141 taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct
     4201 gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa
     4261 tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac
     4321 caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt
     4381 ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag
     4441 ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga
     4501 gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag
     4561 cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag
     4621 ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta
     4681 tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt
     4741 gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt
     4801 gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta
     4861 tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc
     4921 ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc
     4981 taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca
     5041 cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg
     5101 cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg
     5161 ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg
     5221 cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg
     5281 cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat
     5341 cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc
     5401 catcgacacc atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc
     5461 cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc
     5521 ccggtttcag aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc
     5581 cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa
     5641 cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaaca aaaggccggc
     5701 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc
     5761 agattacgct tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta
     5821 tgcctaaagc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     5881 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     5941 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     6001 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     6061 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg ccatatgctg
     6121 gctgccatga acaaaggtgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     6181 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     6241 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     6301 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatccc
     6361 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6421 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6481 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6541 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
     6601 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6661 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6721 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
     6781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6901 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7321 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7561 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7921 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7981 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8041 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8101 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8161 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8221 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8281 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8341 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8401 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8461 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8521 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8581 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8641 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8701 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8761 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8821 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8881 gtgccacctg
//
