LOCUS       sequence-215669-       13030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for constitutive expression of BE4GamRA in mammalian 
            cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 13030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE4GamRA-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..13030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             671..1354
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             join(1403..1426,1427..1429,1430..5503)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1403..5503
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5516..5764
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5777..5797
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5798..6046
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6059..6079
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6095..6151
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6152..6751
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6152..6171)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6648..6668
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6767..7355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6820..6840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7238..7249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7427..7660
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7738..7859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7775..7794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7829..7848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7899..8034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8054..8073)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8055..8073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8083..8100)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8083..8099)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8092..8114)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8241..8696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8328..8347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8538..8559
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8722..8826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8827..8895,8896..9687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9045..9064)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9858..10446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10347..10366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10600..10617
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10734..10755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10770..10775,10776..10793,10794..10800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10808..10824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10813..10835
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10832..10848
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10832..10848
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10867..10887
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10869..10887
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10915..11141
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11142..11322
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11369..11494
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11987..12220
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12405..12449
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12633..12750
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        12819..13030
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac gatgacgata agatggcccc
       61 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgcaaaac ctgcaaagag
      121 aattaaatcc gcagcagcag cctacgtgcc tcaaaaccgg gatgccgtta tcacagatat
      181 aaaaagaatc ggtgatttgc agcgcgaagc aagccgcttg gagaccgaaa tgaatgatgc
      241 catcgcagag atcactgaga aatttgctgc ccgcatagca ccaatcaaga ctgacatcga
      301 gacactcagt aagggcgtgc aaggctggtg cgaggctaat cgggacgagt tgaccaacgg
      361 ggggaaggtg aaaaccgcca atcttgtgac tggcgatgtc tcctggcgag tgagaccacc
      421 aagcgtaagc atccgaggca tggacgctgt gatggaaaca ttggaaaggc tcggcctgca
      481 aaggtttatc agaacaaagc aggaaataaa taaggaagcc atcctccttg agccaaaagc
      541 cgttgctggg gtagccggaa ttactgttaa gtctggtatc gaggatttca gtatcatacc
      601 cttcgagcag gaagccggca ttagcggaag tgaaacaccc ggtacctcag agagcgcaac
      661 tcctgagagt agctcagaga ctggcccagt ggctgtggac cccacattga gacggcggat
      721 cgagccccat gagtttgagg tattcttcga tccgagagag ctccgcaagg agacctgcct
      781 gctttacgaa attaattggg ggggccggca ctccatttgg cgacatacat cacagaacac
      841 taacaagcac gtcgaagtca acttcatcga gaagttcacg acagaaagat atttctgtcc
      901 gaacacaagg tgcagcatta cctggtttct cagctggagc ccatgcggcg aatgtagtag
      961 ggccatcact gaattcctgt caaggtatcc ccacgtcact ctgtttattt acatcgcaag
     1021 gctgtaccac cacgctgacc cccgcaatcg acaaggcctg cgggatttga tctcttcagg
     1081 tgtgactatc caaattatga ctgagcagga gtcaggatac tgctggagaa actttgtgaa
     1141 ttatagcccg agtaatgaag cccactggcc taggtatccc catctgtggg tacgactgta
     1201 cgttcttgaa ctgtactgca tcatactggg cctgcctcct tgtctcaaca ttctgagaag
     1261 gaagcagcca cagctgacat tctttaccat cgctcttcag tcttgtcatt accagcgact
     1321 gcccccacac attctctggg ccaccgggtt gaaaagcggc agcgagactc ccgggacctc
     1381 agagtccgcc acacccgaaa gtgacaagaa gtacagcatc ggcctggcca tcggcaccaa
     1441 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     1501 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     1561 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     1621 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     1681 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     1741 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     1801 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     1861 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     1921 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     1981 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     2041 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     2101 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     2161 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     2221 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     2281 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     2341 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     2401 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     2461 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     2521 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     2581 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     2641 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     2701 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     2761 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     2821 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     2881 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     2941 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     3001 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     3061 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     3121 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     3181 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     3241 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     3301 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     3361 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     3421 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     3481 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     3541 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     3601 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     3661 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     3721 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     3781 gatcctgaaa gaacacccag tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     3841 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     3901 cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     3961 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     4021 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     4081 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     4141 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     4201 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     4261 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     4321 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     4381 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     4441 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     4501 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     4561 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     4621 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     4681 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     4741 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     4801 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     4861 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     4921 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     4981 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     5041 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     5101 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     5161 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     5221 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     5281 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     5341 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     5401 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     5461 cctgtacgag acacggatcg acctgtctca gctgggaggc gattcaggcg gatctactaa
     5521 tctgtcagat attattgaaa aggagaccgg taagcaactg gttatccagg aatccatcct
     5581 catgctccca gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg atatactcgt
     5641 gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg cttctgacta gcgacgcccc
     5701 tgaatacaag ccttgggctc tggtcataca ggatagcaac ggtgagaaca agattaagat
     5761 gctctctggt ggttctccca agaagaagag gaaagtcaca aatctctctg acatcataga
     5821 gaaggagaca gggaaacaac tcgtaataca agagtccatt cttatgctcc ctgaggaggt
     5881 ggaagaagtt atcggcaaca aaccagagag tgacattctg gtccataccg cctacgatga
     5941 aagcacagac gagaacgtta tgttgctcac ttctgacgct ccagaataca aaccttgggc
     6001 actcgtcatt caggacagca acggcgagaa caagatcaaa atgcttagcg ggggcagccc
     6061 caaaaaaaag aggaaggtcg ctagcggcag cggcgccacc aacttcagcc tgctgaagca
     6121 ggccggcgac gtggaggaga accccggccc catgaccgag tacaagccca cggtgcgcct
     6181 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     6241 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     6301 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6361 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6421 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6481 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggagt
     6541 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6601 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6661 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6721 ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa gtcgacaatc aacctctgga
     6781 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6841 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6901 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6961 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7021 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7081 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7141 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7201 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7261 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7321 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     7381 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7441 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7501 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7561 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7621 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     7681 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     7741 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7801 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7861 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7921 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7981 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     8041 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     8101 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     8161 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     8221 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     8281 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     8341 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     8401 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     8461 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     8521 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     8581 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     8641 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     8701 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     8761 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     8821 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     8881 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8941 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9001 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9061 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9121 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9181 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9241 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9301 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9361 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9421 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9481 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9541 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9601 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9661 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9721 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     9781 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9841 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9901 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9961 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10021 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10081 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10141 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10201 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10261 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10321 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10381 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10441 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10501 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    10561 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    10621 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    10681 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    10741 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    10801 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
    10861 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
    10921 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
    10981 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
    11041 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
    11101 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
    11161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    11221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    11281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
    11341 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
    11401 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    11461 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    11521 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    11581 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    11641 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    11701 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    11761 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    11821 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    11881 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    11941 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    12001 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    12061 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    12121 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    12181 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    12241 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    12301 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    12361 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
    12421 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    12481 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    12541 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    12601 acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg gattgggggg
    12661 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    12721 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    12781 ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
    12841 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
    12901 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
    12961 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
    13021 cagaacacag
//
